ID   LPMG_SALT1              Reviewed;          17 AA.
AC   P0CI69;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=mgtA leader peptide;
DE   AltName: Full=Regulatory leader peptide for mgtA;
GN   Name=mgtL; OrderedLocusNames=STM14_5348.1;
OS   Salmonella typhimurium (strain 14028s / SGSC 2262).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=588858;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=19897643; DOI=10.1128/jb.01233-09;
RA   Jarvik T., Smillie C., Groisman E.A., Ochman H.;
RT   "Short-term signatures of evolutionary change in the Salmonella enterica
RT   serovar typhimurium 14028 genome.";
RL   J. Bacteriol. 192:560-567(2010).
RN   [2]
RP   FUNCTION IN TRANSCRIPTION OF MGTA, AND MUTAGENESIS OF PRO-3; 5-PRO--PRO-9;
RP   7-PRO--ARG-17; 10-ARG--ARG-17; 13-ILE--ARG-17 AND 14-PHE--ARG-17.
RX   PubMed=20813261; DOI=10.1016/j.cell.2010.07.046;
RA   Park S.Y., Cromie M.J., Lee E.J., Groisman E.A.;
RT   "A bacterial mRNA leader that employs different mechanisms to sense
RT   disparate intracellular signals.";
RL   Cell 142:737-748(2010).
CC   -!- FUNCTION: Makes mgtA transcription sensitive to intracellular proline
CC       levels. At low levels of proline this proline-rich protein cannot be
CC       fully translated, and stem loop 'C' forms in the mgtA 5' UTR which
CC       permits transcription of the downstream mgtA gene. Osmotic shock
CC       induction (0.3 M NaCl) also depends on mgtL translation. The distance
CC       between the mtgL-translating ribosome and the nucleotides which form
CC       the downstream stem loop 'C' is critical for mgtL-mediated
CC       transcription of mgtA. Cotranscribed with mgtA.
CC       {ECO:0000269|PubMed:20813261}.
CC   -!- SIMILARITY: Belongs to the MgtL family. {ECO:0000305}.
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DR   EMBL; CP001363; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P0CI69; -.
DR   Proteomes; UP000002695; Chromosome.
DR   InterPro; IPR031434; MGTL.
DR   Pfam; PF17059; MGTL; 1.
PE   1: Evidence at protein level;
KW   Leader peptide.
FT   CHAIN           1..17
FT                   /note="mgtA leader peptide"
FT                   /id="PRO_0000403460"
FT   MUTAGEN         3..9
FT                   /note="PEPTPLP->LETTHLL: Increases mgtA transcription. Loss
FT                   of response to osmotic shock."
FT   MUTAGEN         3
FT                   /note="P->L: No change in downstream mgtA transcription
FT                   under limiting proline levels."
FT                   /evidence="ECO:0000269|PubMed:20813261"
FT   MUTAGEN         4..17
FT                   /note="Missing: Significantly increases transcription of
FT                   mgtA."
FT   MUTAGEN         5..9
FT                   /note="PTPLP->TTHLL: Increases mgtA transcription."
FT                   /evidence="ECO:0000269|PubMed:20813261"
FT   MUTAGEN         7..17
FT                   /note="Missing: Increases transcription of mgtA."
FT                   /evidence="ECO:0000269|PubMed:20813261"
FT   MUTAGEN         10..17
FT                   /note="Missing: Increases transcription of mgtA."
FT                   /evidence="ECO:0000269|PubMed:20813261"
FT   MUTAGEN         13..17
FT                   /note="Missing: Moderately increases transcription of
FT                   mgtA."
FT                   /evidence="ECO:0000269|PubMed:20813261"
FT   MUTAGEN         14..17
FT                   /note="Missing: No effect on transcription of mgtA."
FT                   /evidence="ECO:0000269|PubMed:20813261"
SQ   SEQUENCE   17 AA;  2172 MW;  6436CE7981014720 CRC64;
     MDPEPTPLPR WRIFLFR