ID   LPOA_ALIF1              Reviewed;         603 AA.
AC   Q5E2P0;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Penicillin-binding protein activator LpoA {ECO:0000255|HAMAP-Rule:MF_01890};
DE            Short=PBP activator LpoA {ECO:0000255|HAMAP-Rule:MF_01890};
DE   Flags: Precursor;
GN   Name=lpoA {ECO:0000255|HAMAP-Rule:MF_01890}; OrderedLocusNames=VF_2211;
OS   Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=312309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700601 / ES114;
RX   PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA   Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA   Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA   Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT   "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT   pathogenic congeners.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC   -!- FUNCTION: Regulator of peptidoglycan synthesis that is essential for
CC       the function of penicillin-binding protein 1A (PBP1a).
CC       {ECO:0000255|HAMAP-Rule:MF_01890}.
CC   -!- SUBUNIT: Interacts with PBP1a. {ECO:0000255|HAMAP-Rule:MF_01890}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01890}; Lipid-anchor {ECO:0000255|HAMAP-Rule:MF_01890};
CC       Periplasmic side {ECO:0000255|HAMAP-Rule:MF_01890}.
CC   -!- SIMILARITY: Belongs to the LpoA family. {ECO:0000255|HAMAP-
CC       Rule:MF_01890}.
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DR   EMBL; CP000020; AAW86706.1; -; Genomic_DNA.
DR   RefSeq; WP_011262640.1; NC_006840.2.
DR   RefSeq; YP_205594.1; NC_006840.2.
DR   AlphaFoldDB; Q5E2P0; -.
DR   SMR; Q5E2P0; -.
DR   STRING; 312309.VF_2211; -.
DR   EnsemblBacteria; AAW86706; AAW86706; VF_2211.
DR   KEGG; vfi:VF_2211; -.
DR   PATRIC; fig|312309.11.peg.2250; -.
DR   eggNOG; COG3107; Bacteria.
DR   HOGENOM; CLU_026091_1_0_6; -.
DR   OMA; MRLYAMG; -.
DR   OrthoDB; 776281at2; -.
DR   Proteomes; UP000000537; Chromosome I.
DR   GO; GO:0031241; C:periplasmic side of cell outer membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0030234; F:enzyme regulator activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 1.
DR   HAMAP; MF_01890; LpoA; 1.
DR   InterPro; IPR007443; LpoA.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   PANTHER; PTHR38038; PTHR38038; 1.
DR   Pfam; PF04348; LppC; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Cell shape; Lipoprotein; Membrane; Palmitate;
KW   Peptidoglycan synthesis; Reference proteome; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01890"
FT   CHAIN           27..603
FT                   /note="Penicillin-binding protein activator LpoA"
FT                   /id="PRO_0000405950"
FT   LIPID           27
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01890"
FT   LIPID           27
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01890"
SQ   SEQUENCE   603 AA;  67947 MW;  BEE1AC929ADAD949 CRC64;
     MANMTPRKNS VTRLIAPVAL ALTLAACSSS PKAPDRLDIT QSPTETSNAY ILKADQQQGA
     LEADFLIMAL KAAVQEQNFD LADKLFTRLA TMQLSPAQTA EMQLAHAKML KSQSQYEDAL
     KTLNFEAWWK LENSQWVEYH KLRHELYLLS GDNLNSAREL IELEPFTAED QKAQLWTQVW
     TSVSSLNSTA LEEVKLDETE TNLHGWVQLA TYLDTLKHSP MRLQETLNEW LLANPTHPAA
     TYTPQVILDI LALEIVRPEN VALLLPLSGR FGPQGIRVRD GFINAMMEDK ERDEFTKLKV
     IDTQATSMAE IMTTLEKEQI QFVVGPLVRS KIEEFQSLNS TEIAQLALNI PSEIDTDINS
     CYFTLSPEQE AEQAAVHLFK QGFKHPLYLA PQGTMGERLS QAFSDKWFQL TAKRPSISYF
     GSKAQLQQKV NSVFGIESSQ ARIYQMNALA SMELEAQPRS RRDIDAVYMV AKSSELVLLK
     PFIEVAINPG IKPPKLYASS RSNSGRQTQL VEIKGIEFSD IPLLTNENHS FKAQYDELWP
     KSSNGETRLH ALGMDAYQLV AELPQMKAVD NYRMEGKTGE LSLNQECVIQ RKMSWAVHGE
     ETE