ID   LPOA_PANAM              Reviewed;         677 AA.
AC   D4GNT6;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-MAR-2011, sequence version 2.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Penicillin-binding protein activator LpoA {ECO:0000255|HAMAP-Rule:MF_01890};
DE            Short=PBP activator LpoA {ECO:0000255|HAMAP-Rule:MF_01890};
DE   Flags: Precursor;
GN   Name=lpoA {ECO:0000255|HAMAP-Rule:MF_01890}; OrderedLocusNames=PANA_3474;
OS   Pantoea ananatis (strain LMG 20103).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea.
OX   NCBI_TaxID=706191;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 20103;
RX   PubMed=20348253; DOI=10.1128/jb.00060-10;
RA   De Maayer P., Chan W.Y., Venter S.N., Toth I.K., Birch P.R., Joubert F.,
RA   Coutinho T.A.;
RT   "Genome sequence of Pantoea ananatis LMG20103, the causative agent of
RT   Eucalyptus blight and dieback.";
RL   J. Bacteriol. 192:2936-2937(2010).
CC   -!- FUNCTION: Regulator of peptidoglycan synthesis that is essential for
CC       the function of penicillin-binding protein 1A (PBP1a).
CC       {ECO:0000255|HAMAP-Rule:MF_01890}.
CC   -!- SUBUNIT: Interacts with PBP1a. {ECO:0000255|HAMAP-Rule:MF_01890}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01890}; Lipid-anchor {ECO:0000255|HAMAP-Rule:MF_01890};
CC       Periplasmic side {ECO:0000255|HAMAP-Rule:MF_01890}.
CC   -!- SIMILARITY: Belongs to the LpoA family. {ECO:0000255|HAMAP-
CC       Rule:MF_01890}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ADD78641.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP001875; ADD78641.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_041457821.1; NC_013956.2.
DR   AlphaFoldDB; D4GNT6; -.
DR   SMR; D4GNT6; -.
DR   STRING; 706191.PANA_3474; -.
DR   EnsemblBacteria; ADD78641; ADD78641; PANA_3474.
DR   KEGG; pam:PANA_3474; -.
DR   eggNOG; COG3107; Bacteria.
DR   HOGENOM; CLU_026091_1_1_6; -.
DR   OMA; MRLYAMG; -.
DR   OrthoDB; 776281at2; -.
DR   BioCyc; PANA706191:PANA_RS17630-MON; -.
DR   Proteomes; UP000001702; Chromosome.
DR   GO; GO:0031241; C:periplasmic side of cell outer membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0030234; F:enzyme regulator activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 1.
DR   HAMAP; MF_01890; LpoA; 1.
DR   InterPro; IPR007443; LpoA.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   PANTHER; PTHR38038; PTHR38038; 1.
DR   Pfam; PF04348; LppC; 2.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Cell shape; Lipoprotein; Membrane; Palmitate;
KW   Peptidoglycan synthesis; Reference proteome; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01890"
FT   CHAIN           27..677
FT                   /note="Penicillin-binding protein activator LpoA"
FT                   /id="PRO_0000405938"
FT   REGION          309..359
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           27
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01890"
FT   LIPID           27
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01890"
SQ   SEQUENCE   677 AA;  72482 MW;  1982C362A64A74E4 CRC64;
     MLPSKIVRHK AGRFVPVLLA GLILAACSGQ GPKSQSVDIQ APVTNNSAYY LQQVQQSSDD
     SKTDWQLLAI RALLKEGKYP QAAQAIGELP NQLSDKQQQE LLLLKAQSLV AQQKIADAQP
     ILSQVKTSEL SQDQLARYYG LQIASAQGKT SLALLRAYIA QEPLLKGDER QQNIDATWQA
     LTQLSQQDMN SLVINADENI LQGWLDLLTA WHANAQDANM LKSAIKDWQT RYPDNPAAKT
     LPTQLSQVQN FTKASTSTIA LLLPLNGQAQ MFASAIQKGF NDAKNGTLAT APQATPGSAQ
     DPIAINQQQP ADANAVVSPS ANPAAAQQSG TAQQPATTQQ QPQQQPAAEP ASNAQVKVYD
     TSSQPIAQVM QQAQQDGATL VVGPLLKNNV ETVANSQTPL NVLALNEPEQ IQNHPNMCYF
     ALSPEDEARD AAHHIWDQGK RQPLLLVPRN GLGDRVTAAF TKEWQSLGGG TVLQQRFGSV
     SELKQGINSG AGISMSGTPV VMPSSSQPQS VSVAGLNIPA PQTSAPAASS GGAIDAAYIV
     STQDELQLIK PMISMRTGSR SNVALYASSR SAQAGAGPDF RLEMEGLQFS DIPLLSGANP
     ALMQQAAKSF NNDYSLVRLY AMGIDAWTLS NHFNQMRQVP GFSLDGNTGK LSATADCVIN
     RKLTWNQYRQ GNIVPAS