ID   LPOA_PECPW              Reviewed;         672 AA.
AC   D0KEP3;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Penicillin-binding protein activator LpoA {ECO:0000255|HAMAP-Rule:MF_01890};
DE            Short=PBP activator LpoA {ECO:0000255|HAMAP-Rule:MF_01890};
DE   Flags: Precursor;
GN   Name=lpoA {ECO:0000255|HAMAP-Rule:MF_01890}; OrderedLocusNames=Pecwa_0314;
OS   Pectobacterium parmentieri (strain WPP163) (Pectobacterium wasabiae (strain
OS   WPP163)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=561231;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WPP163;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA   Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA   Balakrishnan V., Glasner J., Perna N.T.;
RT   "Complete sequence of Pectobacterium wasabiae WPP163.";
RL   Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Regulator of peptidoglycan synthesis that is essential for
CC       the function of penicillin-binding protein 1A (PBP1a).
CC       {ECO:0000255|HAMAP-Rule:MF_01890}.
CC   -!- SUBUNIT: Interacts with PBP1a. {ECO:0000255|HAMAP-Rule:MF_01890}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01890}; Lipid-anchor {ECO:0000255|HAMAP-Rule:MF_01890};
CC       Periplasmic side {ECO:0000255|HAMAP-Rule:MF_01890}.
CC   -!- SIMILARITY: Belongs to the LpoA family. {ECO:0000255|HAMAP-
CC       Rule:MF_01890}.
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DR   EMBL; CP001790; ACX86167.1; -; Genomic_DNA.
DR   RefSeq; WP_012822118.1; NC_013421.1.
DR   AlphaFoldDB; D0KEP3; -.
DR   SMR; D0KEP3; -.
DR   KEGG; pwa:Pecwa_0314; -.
DR   eggNOG; COG3107; Bacteria.
DR   HOGENOM; CLU_026091_1_1_6; -.
DR   OMA; MRLYAMG; -.
DR   GO; GO:0031241; C:periplasmic side of cell outer membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0030234; F:enzyme regulator activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 1.
DR   HAMAP; MF_01890; LpoA; 1.
DR   InterPro; IPR007443; LpoA.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   PANTHER; PTHR38038; PTHR38038; 1.
DR   Pfam; PF04348; LppC; 2.
DR   SUPFAM; SSF53822; SSF53822; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Cell shape; Lipoprotein; Membrane; Palmitate;
KW   Peptidoglycan synthesis; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01890"
FT   CHAIN           27..672
FT                   /note="Penicillin-binding protein activator LpoA"
FT                   /id="PRO_0000405940"
FT   REGION          298..336
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        301..336
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           27
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01890"
FT   LIPID           27
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01890"
SQ   SEQUENCE   672 AA;  72058 MW;  AA99181BCFE2D63F CRC64;
     MLPFHLVRTQ AGRVIPVLLA ALFLAGCPSH APQSPPPEVQ GKADASSDYY LQQMQQSSDN
     NKADWQLLAI RSLLQEGKAP QASQQFNTLP EKLSAAQKQE QQLLSAELSA AQNNMDAAKA
     ALSQLDVGAL SEQQKSRYYQ TQIKTAQGRP SIELLRAYIA QEPLLKGDEH QMNLDQTWLA
     LTQMSPQESG ALLINADENV LQGWVDLLNN YQNNRESPDQ LQSAIQDWKT RYPHHPAAKN
     LPMQLNQVIN YQPSSVSSIA LLLPLNGQAQ VFANAIQQGF NAAKNGQIAT AAVSAPVAPP
     TDTAQAGQVT PSSDGQNAQS PAPYSDQAVA STTPAPAAQA TSAGLSSSLP VKVYDTSSQP
     LANILTQAQQ DGASLVIGPL LKNEVDQLAS NPSPLNILAL NQPERVENSP NICYFALSPE
     DEARDAAKFI HQQGKQQPLV LAPRGALGDR IVNAFAQAWN QQSGTSALQQ RFGNSAELKQ
     AINSGAGLSL NGQPVNVSQQ QAQAGTTIGG LTIPSQVQPT ASSSVSGNID AVYIIATPDE
     LALIKPMIDM RTSSRARPAL YASSRSFQAG LGPDFRLEME GLQFSDIPLL AGANPALMQQ
     VSSQFKNDYS LVRLYAMGMD AWTLASHFGE MRQIPGHQIS GATGMLSAGP DCTINRQLTW
     QQYRQGQLVP VL