5HT3A_CAVPO
ID 5HT3A_CAVPO Reviewed; 490 AA.
AC O70212; O70213;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=5-hydroxytryptamine receptor 3A;
DE Short=5-HT3-A;
DE Short=5-HT3A;
DE AltName: Full=5-hydroxytryptamine receptor 3;
DE Short=5-HT-3;
DE Short=5-HT3R;
DE AltName: Full=Serotonin receptor 3A;
DE AltName: Full=Serotonin-gated ion channel receptor;
DE Flags: Precursor;
GN Name=HTR3A; Synonyms=5HT3R, HTR3;
OS Cavia porcellus (Guinea pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC Cavia.
OX NCBI_TaxID=10141 {ECO:0000312|EMBL:AAC06136.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE
RP SPECIFICITY, AND PHARMACOLOGICAL CHARACTERIZATION.
RC TISSUE=Small intestine;
RX PubMed=9463477; DOI=10.1124/mol.53.2.202;
RA Lankiewicz S., Lobitz N., Wetzel C.H.R., Rupprecht R., Gisselmann G.,
RA Hatt H.;
RT "Molecular cloning, functional expression, and pharmacological
RT characterization of 5-hydroxytryptamine3 receptor cDNA and its splice
RT variants from guinea pig.";
RL Mol. Pharmacol. 53:202-212(1998).
CC -!- FUNCTION: This is one of the several different receptors for 5-
CC hydroxytryptamine (serotonin), a biogenic hormone that functions as a
CC neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-
CC gated ion channel, which when activated causes fast, depolarizing
CC responses in neurons. It is a cation-specific, but otherwise relatively
CC nonselective, ion channel. {ECO:0000269|PubMed:9463477}.
CC -!- SUBUNIT: Forms pentahomomeric complex as well as pentaheteromeric
CC complex with HTR3B or HTR3C or HTR3D or HTR3E; homomeric complex is
CC functional but exhibits low conductance with modified voltage
CC dependence, and decreased agonist and antagonist affinity. Interacts
CC with RIC3 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC protein. Cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=5-HT3R-L;
CC IsoId=O70212-1; Sequence=Displayed;
CC Name=2; Synonyms=5-HT3R-S;
CC IsoId=O70212-2; Sequence=VSP_000077;
CC -!- TISSUE SPECIFICITY: Expressed in cortex, intestine and liver. Not
CC expressed in muscle or spleen. {ECO:0000269|PubMed:9463477}.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; AF006461; AAC06136.1; -; mRNA.
DR EMBL; AF006462; AAC06137.1; -; mRNA.
DR RefSeq; NP_001166178.1; NM_001172707.1. [O70212-1]
DR AlphaFoldDB; O70212; -.
DR SMR; O70212; -.
DR STRING; 10141.ENSCPOP00000009728; -.
DR BindingDB; O70212; -.
DR ChEMBL; CHEMBL3666; -.
DR DrugCentral; O70212; -.
DR iPTMnet; O70212; -.
DR GeneID; 100135547; -.
DR KEGG; cpoc:100135547; -.
DR CTD; 3359; -.
DR eggNOG; KOG3645; Eukaryota.
DR InParanoid; O70212; -.
DR OrthoDB; 611108at2759; -.
DR Proteomes; UP000005447; Unassembled WGS sequence.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; IDA:UniProtKB.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005230; F:extracellular ligand-gated ion channel activity; IEA:InterPro.
DR GO; GO:0004890; F:GABA-A receptor activity; IDA:UniProtKB.
DR GO; GO:0006812; P:cation transport; IDA:UniProtKB.
DR Gene3D; 1.20.58.390; -; 1.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR008132; 5HT3_rcpt.
DR InterPro; IPR008133; 5HT3_rcpt_A.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR01709; 5HT3ARECEPTR.
DR PRINTS; PR01708; 5HT3RECEPTOR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Disulfide bond; Glycoprotein;
KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane;
KW Postsynaptic cell membrane; Receptor; Reference proteome; Signal; Synapse;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..490
FT /note="5-hydroxytryptamine receptor 3A"
FT /id="PRO_0000000407"
FT TOPO_DOM 20..249
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 271..285
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 307..312
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 334..467
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 489..490
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 401..422
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 426..462
FT /note="HA-stretch"
FT CARBOHYD 33
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 175
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 191
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 162..176
FT /evidence="ECO:0000250"
FT VAR_SEQ 386..391
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:9463477"
FT /id="VSP_000077"
SQ SEQUENCE 490 AA; 55656 MW; EDB32F8597494F61 CRC64;
MVLWLQLALL ALLLPTSLAQ GEVRGKGTAQ AHNSTRPALQ RLSDHLLADY RKSVRPVRDW
RKPTTVSIDA IVYAILSVDE KNQVLTTYIW YRQFWTDEFL QWNPEDFDNI TKLSIPTDSI
WVPDILINEF VDVGKSPNIP YVYVRHQGEV QNYKPLQVVT ACSLDIYNFP FDVQNCSLTF
TSWLHTIQDI NISLWRLPEK VKSDKSVFMN QGEWELLGVL TEFLEFSDRE SRGSFAEMKF
YVVIRRRPLF YAVTLLLPSI FLMIVDIVGF YLPPDSGERV SFKITLLLGY SVFLIIVSDT
LPATAIGTPL ISVYFVVCMA LLVISLAETI LIVRLVHKQD LQQPVPLWLR HLVLERIAGL
LCLGEQLTSH RGPATLQATK TDDFSGSTLL PAMGNHCGPL GGPQDLEKTS RGRGSPPPPP
REASLAMCGL LQELASIRHF LEKREETREV ARDWLRVGSV LDKLLFRVYL LAVLAYSITL
VTLWSVWHYA
