ID   LPPN_MYCTU              Reviewed;         175 AA.
AC   P9WK73; L0TC06; Q50693;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Putative lipoprotein LppN;
DE   Flags: Precursor;
GN   Name=lppN; OrderedLocusNames=Rv2270; ORFNames=MTCY339.40c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DOMAIN.
RX   PubMed=25041568; DOI=10.1111/cbdd.12365;
RA   Ocampo M., Curtidor H., Vanegas M., Patarroyo M.A., Patarroyo M.E.;
RT   "Specific interaction between Mycobacterium tuberculosis lipoprotein-
RT   derived peptides and target cells inhibits mycobacterial entry in vitro.";
RL   Chem. Biol. Drug Des. 84:626-641(2014).
CC   -!- FUNCTION: Probably involved in bacterial recognition and uptake by its
CC       host (human) (PubMed:25041568). {ECO:0000269|PubMed:25041568}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC       Cell surface {ECO:0000269|PubMed:25041568}. Note=Immunoelectron
CC       microscopy indicates this protein is displaced from the cell surface
CC       (PubMed:25041568). {ECO:0000269|PubMed:25041568}.
CC   -!- DOMAIN: Fragments of the mature protein (residues 61-80, 101-120 and
CC       121-140) prevent uptake of M.tuberculosis by a human macrophage-like
CC       cell line (PubMed:25041568). {ECO:0000269|PubMed:25041568}.
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DR   EMBL; AL123456; CCP45051.1; -; Genomic_DNA.
DR   PIR; B70730; B70730.
DR   RefSeq; NP_216786.1; NC_000962.3.
DR   RefSeq; WP_003411668.1; NZ_NVQJ01000008.1.
DR   AlphaFoldDB; P9WK73; -.
DR   STRING; 83332.Rv2270; -.
DR   PaxDb; P9WK73; -.
DR   DNASU; 887448; -.
DR   GeneID; 887448; -.
DR   KEGG; mtu:Rv2270; -.
DR   TubercuList; Rv2270; -.
DR   eggNOG; ENOG5030GWZ; Bacteria.
DR   OMA; IDSNQDR; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           21..175
FT                   /note="Putative lipoprotein LppN"
FT                   /id="PRO_0000018113"
FT   REGION          31..56
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          61..80
FT                   /note="Prevents bacterial uptake by a human macrophage-like
FT                   cell line"
FT                   /evidence="ECO:0000269|PubMed:25041568"
FT   REGION          101..120
FT                   /note="Prevents bacterial uptake by a human macrophage-like
FT                   cell line"
FT                   /evidence="ECO:0000269|PubMed:25041568"
FT   REGION          121..140
FT                   /note="Prevents bacterial uptake by a human macrophage-like
FT                   cell line"
FT                   /evidence="ECO:0000269|PubMed:25041568"
FT   LIPID           21
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           21
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   175 AA;  18081 MW;  C2FB5FB41B7F9B85 CRC64;
     MRLPGRHVLY ALSAVTMLAA CSSNGARGGI ASTNMNPTNP PATAETATVS PTPAPQSART
     ETWINLQVGD CLADLPPADL SRITVTIVDC ATAHSAEVYL RAPVAVDAAV VSMANRDCAA
     GFAPYTGQSV DTSPYSVAYL IDSHQDRTGA DPTPSTVICL LQPANGQLLT GSARR