LPQH_MYCBP
ID LPQH_MYCBP Reviewed; 159 AA.
AC A0A0H3M9Z0;
DT 09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT 16-SEP-2015, sequence version 1.
DT 25-MAY-2022, entry version 25.
DE RecName: Full=Lipoprotein LpqH;
DE AltName: Full=19 kDa lipoprotein antigen;
DE AltName: Full=Putative transporter LpqH;
DE Flags: Precursor;
GN Name=lpqH; OrderedLocusNames=BCG_3822;
OS Mycobacterium bovis (strain BCG / Pasteur 1173P2).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=410289;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BCG / Pasteur 1173P2;
RX PubMed=17372194; DOI=10.1073/pnas.0700869104;
RA Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P.,
RA Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K.,
RA Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C.,
RA Barrell B.G., Parkhill J., Cole S.T.;
RT "Genome plasticity of BCG and impact on vaccine efficacy.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007).
RN [2]
RP SUBCELLULAR LOCATION DURING INFECTION.
RC STRAIN=BCG / Montreal;
RX PubMed=11123323; DOI=10.4049/jimmunol.166.1.447;
RA Neyrolles O., Gould K., Gares M.P., Brett S., Janssen R., O'Gaora P.,
RA Herrmann J.L., Prevost M.C., Perret E., Thole J.E., Young D.;
RT "Lipoprotein access to MHC class I presentation during infection of murine
RT macrophages with live mycobacteria.";
RL J. Immunol. 166:447-457(2001).
RN [3]
RP SUBCELLULAR LOCATION.
RC STRAIN=BCG / Pasteur 1173P2;
RX PubMed=21364279; DOI=10.1172/jci44261;
RA Prados-Rosales R., Baena A., Martinez L.R., Luque-Garcia J., Kalscheuer R.,
RA Veeraraghavan U., Camara C., Nosanchuk J.D., Besra G.S., Chen B.,
RA Jimenez J., Glatman-Freedman A., Jacobs W.R. Jr., Porcelli S.A.,
RA Casadevall A.;
RT "Mycobacteria release active membrane vesicles that modulate immune
RT responses in a TLR2-dependent manner in mice.";
RL J. Clin. Invest. 121:1471-1483(2011).
CC -!- FUNCTION: Might be involved in ligand transport. A host TLR2 agonist,
CC modifies host gene expression in response to pathogen.
CC {ECO:0000250|UniProtKB:P9WK61}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC Host cytoplasm {ECO:0000269|PubMed:11123323}. Extracellular vesicle,
CC bacterial extracellular vesicle {ECO:0000269|PubMed:21364279}.
CC Note=Following infection of mouse macrophage cell line J774A.1 by live
CC (not heat-killed) bacteria the protein is detected in host cytoplasm at
CC 1 hr but has decreased dramatically by 3 hrs (PubMed:11123323). Also
CC present in bacterial extracytoplasmic vesicles, both in mouse
CC macrophages and in culture media (PubMed:21364279).
CC {ECO:0000269|PubMed:11123323, ECO:0000269|PubMed:21364279}.
CC -!- DOMAIN: Forms a U-shaped beta-half-barrel with a large hydrophobic
CC cavity. {ECO:0000250|UniProtKB:P9WK61}.
CC -!- PTM: Modified by Lgt on Cys-22 with an S-linked diacylglycerol with a
CC mixture of C16, C18 and C19 fatty acids, signal peptide is removed by
CC LspA, modifed by Lnt with an amide-linked mixture of C16 and C19 fatty
CC acids. {ECO:0000250|UniProtKB:P9WK61}.
CC -!- SIMILARITY: Belongs to the mycobacterial 19 kDa antigen family.
CC {ECO:0000305}.
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DR EMBL; AM408590; CAL73812.1; -; Genomic_DNA.
DR RefSeq; WP_003420544.1; NC_008769.1.
DR AlphaFoldDB; A0A0H3M9Z0; -.
DR SMR; A0A0H3M9Z0; -.
DR GeneID; 45427763; -.
DR KEGG; mbb:BCG_3822; -.
DR HOGENOM; CLU_117599_0_0_11; -.
DR OMA; CSDMGGN; -.
DR Proteomes; UP000001472; Chromosome.
DR GO; GO:0097691; C:bacterial extracellular vesicle; IDA:UniProtKB.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR008691; LpqH.
DR Pfam; PF05481; Myco_19_kDa; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell membrane; Host cytoplasm; Lipoprotein; Membrane; Palmitate; Signal;
KW Transport; Virulence.
FT SIGNAL 1..21
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 22..159
FT /note="Lipoprotein LpqH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT /id="PRO_0000434896"
FT REGION 24..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 22
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 22
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 159 AA; 15147 MW; CB1A5090E14867BB CRC64;
MKRGLTVAVA GAAILVAGLS GCSSNKSTTG SGETTTAAGT TASPGAASGP KVVIDGKDQN
VTGSVVCTTA AGNVNIAIGG AATGIAAVLT DGNPPEVKSV GLGNVNGVTL GYTSGTGQGN
ASATKDGSHY KITGTATGVD MANPMSPVNK SFEIEVTCS
