LPQS_MYCTU
ID LPQS_MYCTU Reviewed; 130 AA.
AC O53859; I6Y4Y9;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 3.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Lipoprotein LpqS {ECO:0000305};
DE Flags: Precursor;
GN Name=lpqS {ECO:0000312|EMBL:CCP43595.1};
GN OrderedLocusNames=Rv0847 {ECO:0000312|EMBL:CCP43595.1},
GN RVBD_0847 {ECO:0000312|EMBL:AFN48727.1};
GN ORFNames=LH57_04625 {ECO:0000312|EMBL:AIR13580.1},
GN P425_00887 {ECO:0000312|EMBL:KBJ39046.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genome Sequencing Platform;
RA Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21166899; DOI=10.1111/j.1365-2958.2010.07431.x;
RA Festa R.A., Jones M.B., Butler-Wu S., Sinsimer D., Gerads R., Bishai W.R.,
RA Peterson S.N., Darwin K.H.;
RT "A novel copper-responsive regulon in Mycobacterium tuberculosis.";
RL Mol. Microbiol. 79:133-148(2011).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=H37Rv;
RX PubMed=23562345; DOI=10.1016/j.micres.2013.02.007;
RA Sakthi S., Narayanan S.;
RT "The lpqS knockout mutant of Mycobacterium tuberculosis is attenuated in
RT macrophages.";
RL Microbiol. Res. 168:407-414(2013).
RN [7]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=24549843; DOI=10.1128/mbio.00876-13;
RA Shi X., Festa R.A., Ioerger T.R., Butler-Wu S., Sacchettini J.C.,
RA Darwin K.H., Samanovic M.I.;
RT "The copper-responsive RicR regulon contributes to Mycobacterium
RT tuberculosis virulence.";
RL MBio 5:E00876-E00876(2014).
RN [8]
RP DISRUPTION PHENOTYPE.
RC STRAIN=H37Rv;
RX PubMed=26768127; DOI=10.1016/j.vaccine.2015.12.059;
RA Sakthi S., Palaniyandi K., Gupta U.D., Gupta P., Narayanan S.;
RT "Lipoprotein LpqS deficient M. tuberculosis mutant is attenuated for
RT virulence in vivo and shows protective efficacy better than BCG in guinea
RT pigs.";
RL Vaccine 34:735-743(2016).
CC -!- FUNCTION: May play an essential role in M.tuberculosis replication and
CC survival inside the host cell. {ECO:0000269|PubMed:23562345}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC -!- INDUCTION: Repressed by RicR. Induced by copper.
CC {ECO:0000269|PubMed:21166899}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant shows reduced growth in Sauton's
CC minimal media and is highly sensitive to SDS and copper when grown on
CC solid media (PubMed:23562345). Reduced growth in acidic media (pH 5.5)
CC and under hypoxic conditions, with decreased survival in hypoxia
CC (PubMed:26768127). In vitro, the mutant is attenuated for growth in
CC PMA-activated THP-1 (human) cells (PubMed:23562345). Mutant is
CC extremely resistant to copper and shows increased growth in vivo
CC (PubMed:24549843). In vivo infected guinea pigs exhibited a
CC considerably attenuated infection, and a few animals cleared the
CC infection (PubMed:26768127). {ECO:0000269|PubMed:23562345,
CC ECO:0000269|PubMed:24549843, ECO:0000269|PubMed:26768127}.
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DR EMBL; AL123456; CCP43595.1; -; Genomic_DNA.
DR EMBL; CP003248; AFN48727.1; -; Genomic_DNA.
DR EMBL; JLDD01000008; KBJ39046.1; -; Genomic_DNA.
DR EMBL; CP009480; AIR13580.1; -; Genomic_DNA.
DR RefSeq; NP_215362.1; NC_000962.3.
DR RefSeq; WP_003901025.1; NZ_NVQJ01000040.1.
DR AlphaFoldDB; O53859; -.
DR STRING; 83332.Rv0847; -.
DR PaxDb; O53859; -.
DR DNASU; 885051; -.
DR GeneID; 45424813; -.
DR GeneID; 885051; -.
DR KEGG; mtu:Rv0847; -.
DR KEGG; mtv:RVBD_0847; -.
DR PATRIC; fig|83332.111.peg.939; -.
DR TubercuList; Rv0847; -.
DR eggNOG; ENOG5032A5H; Bacteria.
DR HOGENOM; CLU_127068_1_0_11; -.
DR OMA; TRYCRAR; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046688; P:response to copper ion; IDA:MTBBASE.
PE 2: Evidence at transcript level;
KW Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 24..130
FT /note="Lipoprotein LpqS"
FT /id="PRO_0000433100"
FT LIPID 24
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 24
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 130 AA; 13519 MW; CB443C14F32A27BD CRC64;
MVWMRSAIVA VALGVTVAAV AAACWLPQLH RHVAHPNHPL TTSVGSEFVI NTDHGHLVDN
SMPPCPERLA TAVLPRSATP VLLPDVVAAA PGMTAALTDP VAPAARGPPA AQGSVRTGQD
LLTRFCLARR
