ID   LPRF_MYCTO              Reviewed;         261 AA.
AC   P9WK46; L0T6N3; P65314; P71798;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Putative diacylated glycolipid transporter LprF;
DE   AltName: Full=Lipoprotein LprF;
DE   Flags: Precursor;
GN   Name=lprF; OrderedLocusNames=MT1415;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Might be involved in transporting short diacylated
CC       glycolipids to the cell outer membrane (By similarity).
CC       {ECO:0000250|UniProtKB:P65315}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- DOMAIN: Forms a U-shaped beta-half-barrel with a small hydrophobic
CC       cavity which is large enough to hold a single diacylated glycolipid
CC       molecule. {ECO:0000250|UniProtKB:P65315}.
CC   -!- PTM: Modified by Lgt on Cys-39 with an S-linked diacylglycerol with a
CC       mixture of C16, C18 and C19 fatty acids (palmitic, stearic and
CC       tuberculostearic acid respectively), signal peptide is removed by LspA,
CC       modified by Lnt with an amide-linked mixture of C16 and C19 fatty acids
CC       (By similarity). {ECO:0000250|UniProtKB:P9WK47}.
CC   -!- SIMILARITY: Belongs to the LppX/LprAFG lipoprotein family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK45679.1; -; Genomic_DNA.
DR   PIR; E70957; E70957.
DR   RefSeq; WP_003407180.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WK46; -.
DR   SMR; P9WK46; -.
DR   EnsemblBacteria; AAK45679; AAK45679; MT1415.
DR   KEGG; mtc:MT1415; -.
DR   PATRIC; fig|83331.31.peg.1522; -.
DR   HOGENOM; CLU_074100_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR   GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR   CDD; cd16334; LppX-like; 1.
DR   InterPro; IPR029046; LolA/LolB/LppX.
DR   InterPro; IPR009830; LppX/LprAFG.
DR   Pfam; PF07161; LppX_LprAFG; 1.
DR   SUPFAM; SSF89392; SSF89392; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipid transport; Lipid-binding; Lipoprotein; Membrane;
KW   Palmitate; Signal; Transport.
FT   SIGNAL          1..38
FT                   /evidence="ECO:0000255"
FT   CHAIN           39..261
FT                   /note="Putative diacylated glycolipid transporter LprF"
FT                   /id="PRO_0000427717"
FT   REGION          42..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           39
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
FT   LIPID           39
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   261 AA;  26852 MW;  26F99D39A9B756C1 CRC64;
     MNGLISQACG SHRPRRPSSL GAVAILIAAT LFATVVAGCG KKPTTASSPS PGSPSPEAQQ
     ILQDSSKATK GLHSVHVVVT VNNLSTLPFE SVDADVTNQP QGNGQAVGNA KVRMKPNTPV
     VATEFLVTNK TMYTKRGGDY VSVGPAEKIY DPGIILDKDR GLGAVVGQVQ NPTIQGRDAI
     DGLATVKVSG TIDAAVIDPI VPQLGKGGGR LPITLWIVDT NASTPAPAAN LVRMVIDKDQ
     GNVDITLSNW GAPVTIPNPA G