LPRG_MYCS2
ID LPRG_MYCS2 Reviewed; 236 AA.
AC A0QWU8;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Lipoarabinomannan carrier protein LprG;
DE AltName: Full=27 kDa lipoprotein;
DE AltName: Full=Antigen P27;
DE AltName: Full=Lipoprotein LprG {ECO:0000303|PubMed:18156250};
DE AltName: Full=Triacylated glycolipid carrier LprG;
DE AltName: Full=Triacylglyceride transfer protein LprG;
DE Flags: Precursor;
GN Name=lprG {ECO:0000303|PubMed:18156250};
GN OrderedLocusNames=MSMEG_3070, MSMEI_2993;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18156250; DOI=10.1128/jb.01046-07;
RA Farrow M.F., Rubin E.J.;
RT "Function of a mycobacterial major facilitator superfamily pump requires a
RT membrane-associated lipoprotein.";
RL J. Bacteriol. 190:1783-1791(2008).
CC -!- FUNCTION: Probably helps membrane protein MSMEG_3069/MSMEI_2992 (P55)
CC transport triacylglycerides (TAG) across the inner cell membrane into
CC the periplasm and probably ultimately to the outer membrane (By
CC similarity). Required for MSMEG_3069/MSMEI_2992 export activity. Export
CC of ethidium bromide by MSMEG_3069/MSMEI_2992 can be complemented by the
CC equivalent operon from M.tuberculosis (lprG-Rv1410c) (PubMed:18156250).
CC {ECO:0000250|UniProtKB:P9WK45, ECO:0000269|PubMed:18156250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000305}; Lipid-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00303}.
CC -!- DOMAIN: Forms a U-shaped beta-half-barrel with a small hydrophobic
CC cavity able to hold a triacylated lipid or triacylglyceride.
CC {ECO:0000250|UniProtKB:P9WK45}.
CC -!- DISRUPTION PHENOTYPE: Double deletions of lprG-MSMEG_3069/MSMEI_2992
CC operon are more sensitive to ethidium bromide; restoration of wild-type
CC growth is conferred by the M.tuberculosis operon. Cells display
CC abnormal colony morphology and are defective for sliding motility under
CC cabon-limiting conditions (PubMed:18156250).
CC {ECO:0000269|PubMed:18156250}.
CC -!- SIMILARITY: Belongs to the LppX/LprAFG lipoprotein family.
CC {ECO:0000305}.
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DR EMBL; CP000480; ABK70994.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP39457.1; -; Genomic_DNA.
DR RefSeq; WP_003894457.1; NZ_SIJM01000002.1.
DR RefSeq; YP_887386.1; NC_008596.1.
DR AlphaFoldDB; A0QWU8; -.
DR SMR; A0QWU8; -.
DR STRING; 246196.MSMEI_2993; -.
DR PRIDE; A0QWU8; -.
DR EnsemblBacteria; ABK70994; ABK70994; MSMEG_3070.
DR EnsemblBacteria; AFP39457; AFP39457; MSMEI_2993.
DR GeneID; 66734475; -.
DR KEGG; msg:MSMEI_2993; -.
DR KEGG; msm:MSMEG_3070; -.
DR PATRIC; fig|246196.19.peg.3031; -.
DR eggNOG; ENOG50338Y0; Bacteria.
DR OMA; TLTPNKW; -.
DR OrthoDB; 1542347at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR CDD; cd16334; LppX-like; 1.
DR InterPro; IPR029046; LolA/LolB/LppX.
DR InterPro; IPR009830; LppX/LprAFG.
DR Pfam; PF07161; LppX_LprAFG; 1.
DR SUPFAM; SSF89392; SSF89392; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Lipid transport; Lipid-binding;
KW Lipoprotein; Membrane; Palmitate; Reference proteome; Signal; Transport.
FT SIGNAL 1..25
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 26..236
FT /note="Lipoarabinomannan carrier protein LprG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT /id="PRO_5007633152"
FT LIPID 26
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 26
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 236 AA; 24108 MW; 9F1ED754A8831337 CRC64;
MQTRPRFAVQ SLFAILATAA ALVAGCSSSS ETSDAPLPDG AALLQESASK TKTQQSVHLL
LTVQGKVDGL PVEKLDGDLT NAPAVAAEGT ADLIAFGQKI ADAKFVIADG NLYAALTPGD
PLSNYGAAAN IYDVSAILNP DTGLANVLAN FSDATADGRE SINGTEAVRV KGNVSADAVN
KIAPALKADG PVPGTAWITE DDHTLLQAQL EPTPGNSVTM TLSDWGKQVN VTKPAA
