ID   LPRJ_MYCTU              Reviewed;         127 AA.
AC   O33192; F2GK47; I6X205; Q7D851;
DT   11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Putative lipoprotein LprJ;
DE   Flags: Precursor;
GN   Name=lprJ; OrderedLocusNames=Rv1690; ORFNames=LH57_09210;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA   Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA   Monaco A., King S., Sohrabi A.;
RT   "Phylogenetic analysis of Mycobacterial species using whole genome
RT   sequences.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, POSSIBLE INTERACTION WITH KDPD, TOPOLOGY, AND MUTAGENESIS OF
RP   54-ASN--GLY-56.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=12581360; DOI=10.1046/j.1365-2958.2003.03356.x;
RA   Steyn A.J., Joseph J., Bloom B.R.;
RT   "Interaction of the sensor module of Mycobacterium tuberculosis H37Rv KdpD
RT   with members of the Lpr family.";
RL   Mol. Microbiol. 47:1075-1089(2003).
CC   -!- FUNCTION: Overexpression induces expression of sensor protein kdpD gene
CC       at low K(+) concentrations (0 and 250 uM, tested in M.smegatis).
CC       {ECO:0000269|PubMed:12581360}.
CC   -!- SUBUNIT: May interact with sensor protein KdpD.
CC       {ECO:0000305|PubMed:12581360}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255,
CC       ECO:0000305|PubMed:12581360}; Single-pass membrane protein
CC       {ECO:0000255}. Note=Probably also has a lipid-anchor at the mature N-
CC       terminus. Has been suggested to have 2 transmembrane domains
CC       (PubMed:12581360), but bioinformatic evidence does not support this.
CC       {ECO:0000305}.
CC   -!- PTM: Modified by Lgt on Cys-35 with an S-linked diacylglycerol, signal
CC       peptide is removed by LspA, modified by Lnt with amide-linked fatty
CC       acid (By similarity). {ECO:0000250|UniProtKB:P9WK47}.
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DR   EMBL; CP009480; AIR14441.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP44455.1; -; Genomic_DNA.
DR   RefSeq; NP_216206.1; NC_000962.3.
DR   RefSeq; WP_003408378.1; NZ_NVQJ01000010.1.
DR   AlphaFoldDB; O33192; -.
DR   STRING; 83332.Rv1690; -.
DR   PaxDb; O33192; -.
DR   DNASU; 885670; -.
DR   GeneID; 45425659; -.
DR   GeneID; 885670; -.
DR   KEGG; mtu:Rv1690; -.
DR   PATRIC; fig|83332.111.peg.1878; -.
DR   TubercuList; Rv1690; -.
DR   eggNOG; ENOG5032JRP; Bacteria.
DR   HOGENOM; CLU_135573_3_1_11; -.
DR   OMA; AIATYCP; -.
DR   PhylomeDB; O33192; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:MTBBASE.
DR   InterPro; IPR007969; DUF732.
DR   Pfam; PF05305; DUF732; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..34
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           35..127
FT                   /note="Putative lipoprotein LprJ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT                   /id="PRO_0000434609"
FT   TOPO_DOM        35..99
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305|PubMed:12581360"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        121..127
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:12581360"
FT   LIPID           35
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           35
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   MUTAGEN         54..56
FT                   /note="NAG->SAS: Increases KdpD induction at 0 and 250 uM
FT                   K(+)."
FT                   /evidence="ECO:0000269|PubMed:12581360"
SQ   SEQUENCE   127 AA;  13106 MW;  B54064833A27088B CRC64;
     MTAHTHDGTR TWRTGRQATT LLALLAGVFG GAASCAAPIQ ADMMGNAFLT ALTNAGIAYD
     QPATTVALGR SVCPMVVAPG GTFESITSRM AEINGMSRDM ASTFTIVAIG TYCPAVIAPL
     MPNRLQA