ID   LPRN_MYCTU              Reviewed;         384 AA.
AC   I6Y3P1;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Lipoprotein LprN {ECO:0000303|PubMed:24989028};
DE   Flags: Precursor;
GN   Name=lprN; Synonyms=mce4E {ECO:0000312|EMBL:CCP46317.1};
GN   OrderedLocusNames=Rv3495c; ORFNames=LH57_19060;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA   Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA   Monaco A., King S., Sohrabi A.;
RT   "Phylogenetic analysis of Mycobacterial species using whole genome
RT   sequences.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [4]
RP   FUNCTION, AND PALMITOYLATION AT CYS-21.
RC   STRAIN=H37Rv;
RX   PubMed=24989028; DOI=10.1111/2049-632x.12200;
RA   Pasricha R., Saini N.K., Rathor N., Pathak R., Sinha R., Varma-Basil M.,
RA   Mishra K., Brahmachari V., Bose M.;
RT   "The Mycobacterium tuberculosis recombinant LprN protein of mce4 operon
RT   induces Th-1 type response deleterious to protection in mice.";
RL   Pathog. Dis. 72:188-196(2014).
CC   -!- FUNCTION: Stimulates the host (mouse) immune response; lipidated
CC       protein produced in E.coli stimulates T-cell proliferation in mice
CC       previously sensitized with LprN. Spleenocytes from these mice produce
CC       increased amounts of TNF-alpha and IFN-gamma, as well as somewhat
CC       increased nitric oxide levels, upon subsequent challenge with LprN.
CC       Previously sensitized mice infected with M.tuberculosis have an
CC       exacerbated disease response, suggesting this lipoprotein may down-
CC       regulate the host's immune response. {ECO:0000269|PubMed:24989028}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- PTM: Lipidated upon expression in E.coli.
CC       {ECO:0000269|PubMed:24989028}.
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DR   EMBL; AL123456; CCP46317.1; -; Genomic_DNA.
DR   EMBL; CP009480; AIR16289.1; -; Genomic_DNA.
DR   RefSeq; NP_218012.1; NC_000962.3.
DR   RefSeq; WP_003418964.1; NZ_NVQJ01000042.1.
DR   AlphaFoldDB; I6Y3P1; -.
DR   SMR; I6Y3P1; -.
DR   STRING; 83332.Rv3495c; -.
DR   PaxDb; I6Y3P1; -.
DR   PRIDE; I6Y3P1; -.
DR   DNASU; 888364; -.
DR   GeneID; 888364; -.
DR   KEGG; mtu:Rv3495c; -.
DR   PATRIC; fig|83332.111.peg.3893; -.
DR   TubercuList; Rv3495c; -.
DR   eggNOG; COG1463; Bacteria.
DR   HOGENOM; CLU_045966_1_1_11; -.
DR   OMA; LGSMHID; -.
DR   PhylomeDB; I6Y3P1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR005693; Mce.
DR   InterPro; IPR003399; Mce/MlaD.
DR   Pfam; PF02470; MlaD; 1.
DR   TIGRFAMs; TIGR00996; Mtu_fam_mce; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal; Virulence.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           21..384
FT                   /note="Lipoprotein LprN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT                   /id="PRO_0000438419"
FT   LIPID           21
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT                   ECO:0000305|PubMed:24989028"
FT   LIPID           21
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   384 AA;  41293 MW;  D033E406C2D13AD6 CRC64;
     MNRIWLRAII LTASSALLAG CQFGGLNSLP LPGTAGHGEG AYSVTVEMAD VATLPQNSPV
     MVDDVTVGSV AGIVAVQRPD GSFYAAVKLD LDKNVLLPAN AVAKVSQTSL LGSLHVELAP
     PTDRPPTGRL VDGSRITEAN TDRFPTTEEV FSALGVVVNK GNVGALEEII DETHQAVAGR
     QAQFVNLVPR LAELTAGLNR QVHDIIDALD GLNRVSAILA RDKDNLGRAL DTLPDAVRVL
     NQNRDHIVDA FAALKRLTMV TSHVLAETKV DFGEDLKDLY SIVKALNDDR KDFVTSLQLL
     LTFPFPNFGI KQAVRGDYLN VFTTFDLTLR RIGETFFTTA YFDPNMAHMD EILNPPDFLI
     GELANLSGQA ADPFKIPPGT ASGQ