ID   LPSL_RHIME              Reviewed;         341 AA.
AC   O54067;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Probable UDP-glucuronate 4-epimerase {ECO:0000305};
DE            EC=5.1.3.6 {ECO:0000305|PubMed:9765575};
DE   AltName: Full=UDP-glucuronic acid epimerase {ECO:0000303|PubMed:9765575};
GN   Name=lspL {ECO:0000303|PubMed:9765575}; OrderedLocusNames=R01083;
GN   ORFNames=SMc02640;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=41;
RX   PubMed=9765575; DOI=10.1128/jb.180.20.5426-5431.1998;
RA   Kereszt A., Kiss E., Reuhs B.L., Carlson R.W., Kondorosi A., Putnoky P.;
RT   "Novel rkp gene clusters of Sinorhizobium meliloti involved in capsular
RT   polysaccharide production and invasion of the symbiotic nodule: the rkpK
RT   gene encodes a UDP-glucose dehydrogenase.";
RL   J. Bacteriol. 180:5426-5431(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481430; DOI=10.1073/pnas.161294398;
RA   Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA   Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA   Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA   Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA   Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT   "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT   meliloti strain 1021.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucuronate = UDP-alpha-D-galacturonate;
CC         Xref=Rhea:RHEA:11404, ChEBI:CHEBI:57635, ChEBI:CHEBI:58052;
CC         EC=5.1.3.6; Evidence={ECO:0000305|PubMed:9765575};
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC         Evidence={ECO:0000250|UniProtKB:P09147};
CC   -!- DISRUPTION PHENOTYPE: Mutation affects lipopolysaccharide (LPS)
CC       production. {ECO:0000269|PubMed:9765575}.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. {ECO:0000305}.
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DR   EMBL; AJ222661; CAA10917.1; -; Genomic_DNA.
DR   EMBL; AL591688; CAC45662.1; -; Genomic_DNA.
DR   PIR; T46572; T46572.
DR   RefSeq; NP_385189.1; NC_003047.1.
DR   RefSeq; WP_010969032.1; NC_003047.1.
DR   AlphaFoldDB; O54067; -.
DR   SMR; O54067; -.
DR   STRING; 266834.SMc02640; -.
DR   EnsemblBacteria; CAC45662; CAC45662; SMc02640.
DR   GeneID; 61602543; -.
DR   KEGG; sme:SMc02640; -.
DR   PATRIC; fig|266834.11.peg.2491; -.
DR   eggNOG; COG0451; Bacteria.
DR   HOGENOM; CLU_007383_1_7_5; -.
DR   OMA; EPIKVFN; -.
DR   Proteomes; UP000001976; Chromosome.
DR   GO; GO:0050378; F:UDP-glucuronate 4-epimerase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR001509; Epimerase_deHydtase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF01370; Epimerase; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Isomerase; NAD; Reference proteome.
FT   CHAIN           1..341
FT                   /note="Probable UDP-glucuronate 4-epimerase"
FT                   /id="PRO_0000183257"
FT   ACT_SITE        152
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P09147"
FT   CONFLICT        176
FT                   /note="S -> A (in Ref. 1; CAA10917)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   341 AA;  38099 MW;  D678ED2EAFD89395 CRC64;
     MRYLITGTAG FIGFHVAKRL IDEGHFVVGF DGMTPYYDVT LKERRHAILQ RSNGFKAVTA
     MLEDRAALDR AAELAEPEVI IHLAAQAGVR YSLENPKAYV DANLVGSWNM LELAKAIAPK
     HLMLASTSSI YGANEKIPFA EADRADEPMT LYAATKKSME LMAHSYAHLY KVPTTSFRFF
     TVYGPWGRPD MALFKFVDAI HNGRPIDIYG EGRMSRDFTY IDDLVESIVR LSHVPPSEEN
     RVAPEKATDT LSRHAPFRVV NTGGGQPVEL MTFVETVEKA VGRPAIHNML PMQQGDVPRT
     FASPDLLEAL TGFKPSVSVE EGVARFVEWY DQNYRRAHTT V