5HT3A_RAT
ID 5HT3A_RAT Reviewed; 483 AA.
AC P35563;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=5-hydroxytryptamine receptor 3A;
DE Short=5-HT3-A;
DE Short=5-HT3A;
DE AltName: Full=5-hydroxytryptamine receptor 3;
DE Short=5-HT-3;
DE Short=5-HT3R;
DE AltName: Full=Serotonin receptor 3A;
DE AltName: Full=Serotonin-gated ion channel receptor;
DE Flags: Precursor;
GN Name=Htr3a; Synonyms=5ht3, Htr3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RA Miyake A., Mochizuki S., Akuzawa S., Kon G.;
RL Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 22-483.
RX PubMed=7509203; DOI=10.1097/00001756-199311180-00006;
RA Isenberg K.E., Ukhun I.A., Holstad S.G., Jafri S., Uchida U.,
RA Zorumski C.F., Yang J.;
RT "Partial cDNA cloning and NGF regulation of a rat 5-HT3 receptor subunit.";
RL NeuroReport 5:121-124(1993).
RN [3]
RP SUBUNIT, AND INDUCTION.
RX PubMed=10854267; DOI=10.1046/j.1471-4159.2000.0750240.x;
RA Hanna M.C., Davies P.A., Hales T.G., Kirkness E.F.;
RT "Evidence for expression of heteromeric serotonin 5-HT(3) receptors in
RT rodents.";
RL J. Neurochem. 75:240-247(2000).
RN [4]
RP SUBUNIT, AND TISSUE SPECIFICITY.
RX PubMed=12151552; DOI=10.1523/jneurosci.22-15-06732.2002;
RA Morales M., Wang S.-D.;
RT "Differential composition of 5-hydroxytryptamine3 receptors synthesized in
RT the rat CNS and peripheral nervous system.";
RL J. Neurosci. 22:6732-6741(2002).
RN [5]
RP FUNCTION, AND REGION.
RX PubMed=12867984; DOI=10.1038/nature01788;
RA Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A.;
RT "A cytoplasmic region determines single-channel conductance in 5-HT3
RT receptors.";
RL Nature 424:321-324(2003).
CC -!- FUNCTION: This is one of the several different receptors for 5-
CC hydroxytryptamine (serotonin), a biogenic hormone that functions as a
CC neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-
CC gated ion channel, which when activated causes fast, depolarizing
CC responses in neurons. It is a cation-specific, but otherwise relatively
CC nonselective, ion channel. {ECO:0000269|PubMed:12867984}.
CC -!- SUBUNIT: Interacts with RIC3 (By similarity). Forms pentahomomeric
CC complex as well as pentaheteromeric complex with HTR3B; homomeric
CC complex is functional but exhibits low conductance with modified
CC voltage dependence and antagonist affinity. {ECO:0000250,
CC ECO:0000269|PubMed:10854267, ECO:0000269|PubMed:12151552}.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC protein. Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in central and peripheral neurons.
CC {ECO:0000269|PubMed:12151552}.
CC -!- INDUCTION: By nerve growth factor in PC12 cells.
CC {ECO:0000269|PubMed:10854267}.
CC -!- MISCELLANEOUS: The HA-stretch region of HTR3A seems to be responsible
CC for the low conductance of HTR3A homomers compared to that of
CC HTR3A/HTR3B heteromers.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; D49395; BAA08388.1; -; mRNA.
DR EMBL; U01227; AAA52182.1; -; mRNA.
DR PIR; I58179; I58179.
DR RefSeq; NP_077370.2; NM_024394.2.
DR AlphaFoldDB; P35563; -.
DR SMR; P35563; -.
DR ComplexPortal; CPX-277; 5-hydroxytryptamine-3A/B receptor complex.
DR ComplexPortal; CPX-278; 5-hydroxytryptamine-3A receptor complex.
DR STRING; 10116.ENSRNOP00000008965; -.
DR BindingDB; P35563; -.
DR ChEMBL; CHEMBL2411; -.
DR DrugCentral; P35563; -.
DR GuidetoPHARMACOLOGY; 373; -.
DR TCDB; 1.A.9.2.2; the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.
DR GlyGen; P35563; 3 sites.
DR PhosphoSitePlus; P35563; -.
DR PaxDb; P35563; -.
DR GeneID; 79246; -.
DR KEGG; rno:79246; -.
DR UCSC; RGD:61818; rat.
DR CTD; 3359; -.
DR RGD; 61818; Htr3a.
DR VEuPathDB; HostDB:ENSRNOG00000006595; -.
DR eggNOG; KOG3645; Eukaryota.
DR HOGENOM; CLU_018074_5_0_1; -.
DR InParanoid; P35563; -.
DR OMA; FLMLMDI; -.
DR OrthoDB; 611108at2759; -.
DR PhylomeDB; P35563; -.
DR TreeFam; TF315605; -.
DR Reactome; R-RNO-112314; Neurotransmitter receptors and postsynaptic signal transmission.
DR PRO; PR:P35563; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000006595; Expressed in Ammon's horn and 8 other tissues.
DR Genevisible; P35563; RN.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030424; C:axon; IDA:RGD.
DR GO; GO:0032154; C:cleavage furrow; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099055; C:integral component of postsynaptic membrane; IDA:SynGO.
DR GO; GO:0099056; C:integral component of presynaptic membrane; IDA:SynGO.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0043025; C:neuronal cell body; IDA:RGD.
DR GO; GO:0005886; C:plasma membrane; IDA:RGD.
DR GO; GO:1904602; C:serotonin-activated cation-selective channel complex; ISO:RGD.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0051378; F:serotonin binding; ISO:RGD.
DR GO; GO:0022850; F:serotonin-gated cation-selective channel activity; IDA:RGD.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0071363; P:cellular response to growth factor stimulus; IEP:RGD.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0098662; P:inorganic cation transmembrane transport; ISO:RGD.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0032414; P:positive regulation of ion transmembrane transporter activity; IDA:RGD.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0042220; P:response to cocaine; IEP:RGD.
DR GO; GO:0045471; P:response to ethanol; IEP:RGD.
DR GO; GO:0007210; P:serotonin receptor signaling pathway; ISO:RGD.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR Gene3D; 1.20.58.390; -; 1.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR008132; 5HT3_rcpt.
DR InterPro; IPR008133; 5HT3_rcpt_A.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR01709; 5HT3ARECEPTR.
DR PRINTS; PR01708; 5HT3RECEPTOR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Postsynaptic cell membrane; Receptor;
KW Reference proteome; Signal; Synapse; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..483
FT /note="5-hydroxytryptamine receptor 3A"
FT /id="PRO_0000000410"
FT TOPO_DOM 24..246
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..273
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 274..278
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..297
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..307
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..326
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..460
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..480
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 481..483
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 393..414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 419..455
FT /note="HA-stretch"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 175
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 191
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 162..176
FT /evidence="ECO:0000250"
FT CONFLICT 22
FT /note="E -> K (in Ref. 2; AAA52182)"
FT /evidence="ECO:0000305"
FT CONFLICT 306
FT /note="G -> R (in Ref. 2; AAA52182)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 483 AA; 55428 MW; ED85257BBCCF28A4 CRC64;
MPLCIPQVLL ALFLSVLIAQ GEGSRRRATQ AHSTTQPALL RLSDHLLANY KKGVRPVRDW
RKPTLVSIDV IMYAILNVDE KNQVLTTYIW YRQFWTDEFL QWTPEDFDNV TKLSIPTDSI
WVPDILINEF VDVGKSPSIP YVYVHHQGEV QNYKPLQLVT ACSLDIYNFP FDVQNCSLTF
TSWLHTIQDI NISLWRTPEE VRSDKSIFIN QGEWELLGVF TKFQEFSIET SNSYAEMKFY
VVIRRRPLFY AVSLLLPSIF LMVVDIVGFC LPPDSGERVS FKITLLLGYS VFLIIVSDTL
PATAIGTPLI GVYFVVCMAL LVISLAETIF IVQLVHKQDL QRPVPDWLRH LVLDRIAWLL
CLGEQPMAHR PPATFQANKT DDCSAMGNHC SHVGSPQDLE KTSRSRDSPL PPPREASLAV
RGLLQELSSI RHSLEKRDEM REVARDWLRV GYVLDRLLFR IYLLAVLAYS ITLVTLWSIW
HYS
