LPTD_SHIFL
ID LPTD_SHIFL Reviewed; 784 AA.
AC Q83SQ0; Q7C3B4;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=LPS-assembly protein LptD {ECO:0000255|HAMAP-Rule:MF_01411};
DE Flags: Precursor;
GN Name=lptD {ECO:0000255|HAMAP-Rule:MF_01411}; Synonyms=imp, ostA;
GN OrderedLocusNames=SF0051, S0053;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Together with LptE, is involved in the assembly of
CC lipopolysaccharide (LPS) at the surface of the outer membrane.
CC {ECO:0000255|HAMAP-Rule:MF_01411}.
CC -!- SUBUNIT: Component of the lipopolysaccharide transport and assembly
CC complex. Interacts with LptE and LptA. {ECO:0000255|HAMAP-
CC Rule:MF_01411}.
CC -!- INTERACTION:
CC Q83SQ0; Q83LX4: lptE; NbExp=4; IntAct=EBI-16111665, EBI-16111649;
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC Rule:MF_01411}.
CC -!- PTM: Contains two intramolecular disulfide bonds. {ECO:0000255|HAMAP-
CC Rule:MF_01411}.
CC -!- SIMILARITY: Belongs to the LptD family. {ECO:0000255|HAMAP-
CC Rule:MF_01411}.
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DR EMBL; AE005674; AAN41717.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP15597.1; -; Genomic_DNA.
DR RefSeq; NP_706010.1; NC_004337.2.
DR RefSeq; WP_000746145.1; NZ_WPGW01000005.1.
DR PDB; 4Q35; X-ray; 2.39 A; A=1-784.
DR PDBsum; 4Q35; -.
DR AlphaFoldDB; Q83SQ0; -.
DR SMR; Q83SQ0; -.
DR DIP; DIP-61034N; -.
DR IntAct; Q83SQ0; 1.
DR STRING; 198214.SF0051; -.
DR PRIDE; Q83SQ0; -.
DR EnsemblBacteria; AAN41717; AAN41717; SF0051.
DR EnsemblBacteria; AAP15597; AAP15597; S0053.
DR GeneID; 1024572; -.
DR GeneID; 58389317; -.
DR KEGG; sfl:SF0051; -.
DR KEGG; sfx:S0053; -.
DR PATRIC; fig|198214.7.peg.60; -.
DR HOGENOM; CLU_009039_2_0_6; -.
DR OMA; DYSHLDW; -.
DR OrthoDB; 100018at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043165; P:Gram-negative-bacterium-type cell outer membrane assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0015920; P:lipopolysaccharide transport; IEA:InterPro.
DR GO; GO:0010033; P:response to organic substance; IEA:InterPro.
DR HAMAP; MF_01411; LPS_assembly_LptD; 1.
DR InterPro; IPR020889; LipoPS_assembly_LptD.
DR InterPro; IPR007543; LptD_C.
DR InterPro; IPR005653; OstA-like_N.
DR Pfam; PF04453; LptD; 1.
DR Pfam; PF03968; LptD_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Disulfide bond; Membrane;
KW Reference proteome; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01411"
FT CHAIN 25..784
FT /note="LPS-assembly protein LptD"
FT /id="PRO_0000020290"
FT DISULFID 31..724
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01411"
FT DISULFID 173..725
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01411"
FT HELIX 27..32
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 53..55
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 57..61
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 63..65
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 66..77
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 80..85
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 87..90
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 103..106
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 108..113
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 115..127
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 128..130
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 133..145
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 148..157
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 158..161
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 162..171
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 179..190
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 191..194
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 195..205
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 208..219
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 232..236
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 237..239
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 240..244
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 247..252
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 255..264
FT /evidence="ECO:0007829|PDB:4Q35"
FT HELIX 265..267
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 269..280
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 283..293
FT /evidence="ECO:0007829|PDB:4Q35"
FT HELIX 296..301
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 310..321
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 322..324
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 325..336
FT /evidence="ECO:0007829|PDB:4Q35"
FT HELIX 339..342
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 346..348
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 353..365
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 368..379
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 381..383
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 388..400
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 407..424
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 426..441
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 443..461
FT /evidence="ECO:0007829|PDB:4Q35"
FT HELIX 464..468
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 476..491
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 493..497
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 498..500
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 504..517
FT /evidence="ECO:0007829|PDB:4Q35"
FT HELIX 537..540
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 545..550
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 555..567
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 573..586
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 603..615
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 617..629
FT /evidence="ECO:0007829|PDB:4Q35"
FT HELIX 630..632
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 634..647
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 650..659
FT /evidence="ECO:0007829|PDB:4Q35"
FT HELIX 661..667
FT /evidence="ECO:0007829|PDB:4Q35"
FT HELIX 670..674
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 676..680
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 682..693
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 694..696
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 697..706
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 707..710
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 711..722
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 724..740
FT /evidence="ECO:0007829|PDB:4Q35"
FT TURN 741..744
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 745..758
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 760..763
FT /evidence="ECO:0007829|PDB:4Q35"
FT HELIX 769..773
FT /evidence="ECO:0007829|PDB:4Q35"
FT STRAND 775..778
FT /evidence="ECO:0007829|PDB:4Q35"
SQ SEQUENCE 784 AA; 89656 MW; 3C6C1936F8FF2311 CRC64;
MKKRIPTLLA TMIATALYSQ QGLAADLASQ CMLGVPSYDR PLVQGDTNDL PVTINADHAK
GDYPDDAVFT GSVDIMQGNS RLQADEVQLH QKEAPGQPEP VRTVDALGNV HYDDNQVILK
GPKGWANLNT KDTNVWEGDY QMVGRQGRGK ADLMKQRGEN RYTILDNGSF TSCLPGSDTW
SVVGSEIIHD REEQVAEIWN ARFKVGPVPI FYSPYLQLPV GDKRRSGFLI PNAKYTTTNY
FEFYLPYYWN IAPNMDATIT PHYMHRRGNI MWENEFRYLS QAGAGLMELD YLPSDKVYED
EHPNDDSSRR WLFYWNHSGV MDQVWRFNVD YTKVSDPSYF NDFDNKYGSS TDGYATQKFS
VGYAVQNFNA TVSTKQFQVF SEQNTSSYSA EPQLDVNYYQ NDVGPFDTRI YGQAVHFVNT
RDDMPEATRV HLEPTINLPL SNNWGSINTE AKFLATHYQQ TNLDWYNSRN TTKLDESVNR
VMPQFKVDGK MVFERDMEML APGYTQTLEP RAQYLYVPYR DQSDIYNYDS SLLQSDYSGL
FRDRTYGGLD RIASANQVTT GVTSRIYDDA AVERFNISVG QIYYFTESRT GDDNITWEND
DKTGSLVWAG DTYWRISERW GLRGGIQYDT RLDNVATSNS SIEYRRDEDR LVQLNYHYAS
PEYIQATLPK YYSTAEQYKN GISQVGAVAS RPIADRWSIV GAYYYDTNAN KQADSMLGVQ
YSSCCYAIRV GYERKLNGWD NDKQHAVYDN AIGFNIELRG LSSNYGLGTQ EMLRSNILPY
QNTL
