5HT3B_RAT
ID 5HT3B_RAT Reviewed; 437 AA.
AC Q9JJ16; Q9ERJ5;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=5-hydroxytryptamine receptor 3B;
DE Short=5-HT3-B;
DE Short=5-HT3B;
DE AltName: Full=Serotonin receptor 3B;
DE Flags: Precursor;
GN Name=Htr3b;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, AND INDUCTION.
RC STRAIN=Sprague-Dawley;
RX PubMed=10854267; DOI=10.1046/j.1471-4159.2000.0750240.x;
RA Hanna M.C., Davies P.A., Hales T.G., Kirkness E.F.;
RT "Evidence for expression of heteromeric serotonin 5-HT(3) receptors in
RT rodents.";
RL J. Neurochem. 75:240-247(2000).
RN [2]
RP SUBUNIT, AND TISSUE SPECIFICITY.
RX PubMed=12151552; DOI=10.1523/jneurosci.22-15-06732.2002;
RA Morales M., Wang S.-D.;
RT "Differential composition of 5-hydroxytryptamine3 receptors synthesized in
RT the rat CNS and peripheral nervous system.";
RL J. Neurosci. 22:6732-6741(2002).
RN [3]
RP FUNCTION.
RX PubMed=12867984; DOI=10.1038/nature01788;
RA Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A.;
RT "A cytoplasmic region determines single-channel conductance in 5-HT3
RT receptors.";
RL Nature 424:321-324(2003).
CC -!- FUNCTION: This is one of the several different receptors for 5-
CC hydroxytryptamine (serotonin), a biogenic hormone that functions as a
CC neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-
CC gated ion channel, which when activated causes fast, depolarizing
CC responses. It is a cation-specific, but otherwise relatively
CC nonselective, ion channel. {ECO:0000269|PubMed:12867984}.
CC -!- SUBUNIT: Forms pentahomomeric complex as well as pentaheteromeric
CC complex with HTR3B; homomeric complex is functional but exhibits low
CC conductance with modified voltage dependence and antagonist affinity.
CC {ECO:0000269|PubMed:10854267, ECO:0000269|PubMed:12151552}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC Note=Presumably retained within the endoplasmic reticulum unless
CC complexed with HTR3A. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in peripheral neurons, but not in neurons
CC of the central nervous system. {ECO:0000269|PubMed:12151552}.
CC -!- INDUCTION: By nerve growth factor in PC12 cells.
CC {ECO:0000269|PubMed:10854267}.
CC -!- PTM: N-glycosylation is required for membrane localization.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3B sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; AF155044; AAF73283.1; -; mRNA.
DR EMBL; AF303447; AAG30903.1; -; mRNA.
DR RefSeq; NP_071525.1; NM_022189.1.
DR AlphaFoldDB; Q9JJ16; -.
DR SMR; Q9JJ16; -.
DR ComplexPortal; CPX-277; 5-hydroxytryptamine-3A/B receptor complex.
DR STRING; 10116.ENSRNOP00000009574; -.
DR BindingDB; Q9JJ16; -.
DR ChEMBL; CHEMBL2094116; -.
DR DrugCentral; Q9JJ16; -.
DR TCDB; 1.A.9.2.2; the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.
DR GlyGen; Q9JJ16; 3 sites.
DR PaxDb; Q9JJ16; -.
DR GeneID; 58963; -.
DR KEGG; rno:58963; -.
DR UCSC; RGD:61820; rat.
DR CTD; 9177; -.
DR RGD; 61820; Htr3b.
DR eggNOG; KOG3645; Eukaryota.
DR InParanoid; Q9JJ16; -.
DR OrthoDB; 611108at2759; -.
DR PhylomeDB; Q9JJ16; -.
DR Reactome; R-RNO-112314; Neurotransmitter receptors and postsynaptic signal transmission.
DR PRO; PR:Q9JJ16; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0030424; C:axon; IDA:RGD.
DR GO; GO:0009986; C:cell surface; ISO:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; ISO:RGD.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0043025; C:neuronal cell body; IDA:RGD.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:InterPro.
DR GO; GO:1904602; C:serotonin-activated cation-selective channel complex; ISO:RGD.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0022850; F:serotonin-gated cation-selective channel activity; ISO:RGD.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0071363; P:cellular response to growth factor stimulus; IEP:RGD.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0098662; P:inorganic cation transmembrane transport; ISO:RGD.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0032414; P:positive regulation of ion transmembrane transporter activity; IDA:RGD.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0007210; P:serotonin receptor signaling pathway; ISO:RGD.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR Gene3D; 1.20.58.390; -; 1.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR008132; 5HT3_rcpt.
DR InterPro; IPR008134; 5HT3_rcpt_B.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR01710; 5HT3BRECEPTR.
DR PRINTS; PR01708; 5HT3RECEPTOR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Receptor; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..437
FT /note="5-hydroxytryptamine receptor 3B"
FT /id="PRO_0000312291"
FT TOPO_DOM 22..235
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..255
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..266
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..284
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..295
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..324
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 325..410
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..434
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 435..437
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 377..409
FT /note="HA-stretch"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 92
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 151..165
FT /evidence="ECO:0000250"
FT CONFLICT 194
FT /note="K -> E (in Ref. 1; AAG30903)"
FT /evidence="ECO:0000305"
FT CONFLICT 217
FT /note="S -> N (in Ref. 1; AAG30903)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 437 AA; 50336 MW; 633C2F4175FDB55A CRC64;
MILLWSCLLV AVVGILGTAT PQPGNSSLHR LTRQLLQQYH KEVRPVYNWA EATTVYLDLC
VHAVLDVDVQ NQKLKTSMWY REVWNDEFLS WNSSLFDDIQ EISLPLSAIW APDIIINEFV
DVERSPDLPY VYVNSSGTIR NHKPIQVVSA CSLQTYAFPF DIQNCSLTFN SILHTVEDID
LGFLRNQEDI ENDKRSFLND SEWQLLSVTS TYHIRQSSAG DFAQIRFNVV IRRCPLAYVV
SLLIPSIFLM LVDLGSFYLP PNCRARIVFK TNVLVGYTVF RVNMSDEVPR SAGCTSLIGV
FFTVCMALLV LSLSKSILLI KFLYEERHSE QERPLMCLRG DSDANESRLY LRAPCAEDTE
SPVRQEHQVP SDTLKDFWFQ LQSINNSLRT RDQVYQKEVE WLAILCHFDQ LLFRIYLAVL
GLYTVTLCSL WALWSRM
