LPXB_GLOC7
ID LPXB_GLOC7 Reviewed; 384 AA.
AC B7KFS1;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Lipid-A-disaccharide synthase {ECO:0000255|HAMAP-Rule:MF_00392};
DE EC=2.4.1.182 {ECO:0000255|HAMAP-Rule:MF_00392};
GN Name=lpxB {ECO:0000255|HAMAP-Rule:MF_00392};
GN OrderedLocusNames=PCC7424_5044;
OS Gloeothece citriformis (strain PCC 7424) (Cyanothece sp. (strain PCC
OS 7424)).
OC Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC Aphanothecaceae; Gloeothece; Gloeothece citriformis.
OX NCBI_TaxID=65393;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7424;
RX PubMed=21972240; DOI=10.1128/mbio.00214-11;
RA Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H.,
RA Sherman L.A., Pakrasi H.B.;
RT "Novel metabolic attributes of the genus Cyanothece, comprising a group of
RT unicellular nitrogen-fixing Cyanobacteria.";
RL MBio 2:E214-E214(2011).
CC -!- FUNCTION: Condensation of UDP-2,3-diacylglucosamine and 2,3-
CC diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor
CC of lipid A, a phosphorylated glycolipid that anchors the
CC lipopolysaccharide to the outer membrane of the cell.
CC {ECO:0000255|HAMAP-Rule:MF_00392}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-
CC glucosamine = a lipid A disaccharide + H(+) + UDP;
CC Xref=Rhea:RHEA:67828, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:137748, ChEBI:CHEBI:176338, ChEBI:CHEBI:176343;
CC EC=2.4.1.182; Evidence={ECO:0000255|HAMAP-Rule:MF_00392};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00392}.
CC -!- SIMILARITY: Belongs to the LpxB family. {ECO:0000255|HAMAP-
CC Rule:MF_00392}.
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DR EMBL; CP001291; ACK73396.1; -; Genomic_DNA.
DR RefSeq; WP_015956976.1; NC_011729.1.
DR AlphaFoldDB; B7KFS1; -.
DR SMR; B7KFS1; -.
DR STRING; 65393.PCC7424_5044; -.
DR CAZy; GT19; Glycosyltransferase Family 19.
DR EnsemblBacteria; ACK73396; ACK73396; PCC7424_5044.
DR KEGG; cyc:PCC7424_5044; -.
DR eggNOG; COG0763; Bacteria.
DR HOGENOM; CLU_036577_3_0_3; -.
DR OMA; PTVWAWR; -.
DR OrthoDB; 1258510at2; -.
DR UniPathway; UPA00973; -.
DR Proteomes; UP000002384; Chromosome.
DR GO; GO:0008915; F:lipid-A-disaccharide synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00392; LpxB; 1.
DR InterPro; IPR003835; Glyco_trans_19.
DR PANTHER; PTHR30372; PTHR30372; 1.
DR Pfam; PF02684; LpxB; 1.
DR TIGRFAMs; TIGR00215; lpxB; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Lipid A biosynthesis; Lipid biosynthesis;
KW Lipid metabolism; Reference proteome; Transferase.
FT CHAIN 1..384
FT /note="Lipid-A-disaccharide synthase"
FT /id="PRO_1000191472"
SQ SEQUENCE 384 AA; 42842 MW; 8EEF0A16CFEC0BA2 CRC64;
MRIFISTGEV SGDLQGAMLI EALKRQAALK AMDLEIVALG GDRMAETGVS LLGKTPKIAS
IGLIEALPFI MPTWKLQRKA KQYLQENPPD LLILIDYCGP NVAIGKYARK NIPQVPILYY
IAPQAWVWTT NKKTTQDLVN ITDHLLAIFS EEARYFAQKG MSVSWVGHPI LDRMAQAPTR
EEARQKLGIK PDQTAIALLP VSRKQELKYL LPVVCQAAQQ IQEKLPDVQF LIPLALEDYR
STISAMMEEY GLQGTILDGK SLDALAAADL AIAKSGTVNL ELALLNVPQV VVYRLTPLTL
WIAQNILKFS VPFLSPVNLV VMEEVVPELF QERATPEQIV QESLDLLLNP QRRQKTLSDY
QRVREELGEV GVCDRAAQEI LDYV