LPXB_RALSO
ID LPXB_RALSO Reviewed; 390 AA.
AC Q8XZH8;
DT 15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Lipid-A-disaccharide synthase {ECO:0000255|HAMAP-Rule:MF_00392};
DE EC=2.4.1.182 {ECO:0000255|HAMAP-Rule:MF_00392};
GN Name=lpxB {ECO:0000255|HAMAP-Rule:MF_00392}; OrderedLocusNames=RSc1417;
GN ORFNames=RS05275;
OS Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=267608;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
CC -!- FUNCTION: Condensation of UDP-2,3-diacylglucosamine and 2,3-
CC diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor
CC of lipid A, a phosphorylated glycolipid that anchors the
CC lipopolysaccharide to the outer membrane of the cell.
CC {ECO:0000255|HAMAP-Rule:MF_00392}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-
CC glucosamine = a lipid A disaccharide + H(+) + UDP;
CC Xref=Rhea:RHEA:67828, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:137748, ChEBI:CHEBI:176338, ChEBI:CHEBI:176343;
CC EC=2.4.1.182; Evidence={ECO:0000255|HAMAP-Rule:MF_00392};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00392}.
CC -!- SIMILARITY: Belongs to the LpxB family. {ECO:0000255|HAMAP-
CC Rule:MF_00392}.
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DR EMBL; AL646052; CAD15119.1; -; Genomic_DNA.
DR RefSeq; WP_011001366.1; NC_003295.1.
DR AlphaFoldDB; Q8XZH8; -.
DR SMR; Q8XZH8; -.
DR STRING; 267608.RSc1417; -.
DR CAZy; GT19; Glycosyltransferase Family 19.
DR EnsemblBacteria; CAD15119; CAD15119; RSc1417.
DR GeneID; 60500940; -.
DR KEGG; rso:RSc1417; -.
DR eggNOG; COG0763; Bacteria.
DR HOGENOM; CLU_036577_3_0_4; -.
DR OMA; PTVWAWR; -.
DR UniPathway; UPA00973; -.
DR Proteomes; UP000001436; Chromosome.
DR GO; GO:0008915; F:lipid-A-disaccharide synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00392; LpxB; 1.
DR InterPro; IPR003835; Glyco_trans_19.
DR PANTHER; PTHR30372; PTHR30372; 1.
DR Pfam; PF02684; LpxB; 1.
DR TIGRFAMs; TIGR00215; lpxB; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Lipid A biosynthesis; Lipid biosynthesis;
KW Lipid metabolism; Reference proteome; Transferase.
FT CHAIN 1..390
FT /note="Lipid-A-disaccharide synthase"
FT /id="PRO_0000190180"
SQ SEQUENCE 390 AA; 42549 MW; 8AC1151064879FA9 CRC64;
MTQPPAAPAR RIGMAAGEAS GDLLASLLLK GLHARLPRDV AYEGIGGARM AEQGFASHWP
MHKLSVNGYV EVLGQLREIL AIRRELKQNL LADPPMAFIG VDAPDFNFNV EIAMRRAGVP
VVHFVSPSIW AWRAGRIKTI ARAVDHILCL FPFEPEIYAR AGIPATYVGH PLADEIPLEP
DVEGARSRLG LPLGRKVVAV LPGSRNSEVK LLGPTLFAAM ARMQAVEPDL AFVLPAANAM
LRERIDAMRA EHPGLHLWVV DGQSHAAMEA ADVILLASGT ATLEAALYKK PMVITYKVPW
LTAQIMKRKG YLPYVGLPNI LSGRFVVPEL LQDDATPEAL ARETLLQLSD HANAAFLREH
FTQMHLTLKQ NMADIGAQVV VDLLRGRGKL
