5HT4R_HUMAN
ID 5HT4R_HUMAN Reviewed; 388 AA.
AC Q13639; C4WYH4; Q546Q1; Q684M0; Q712M9; Q96KH9; Q96KI0; Q9H199; Q9NY73;
AC Q9UBM6; Q9UBT4; Q9UE22; Q9UE23; Q9UQR6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 03-AUG-2022, entry version 198.
DE RecName: Full=5-hydroxytryptamine receptor 4;
DE Short=5-HT-4;
DE Short=5-HT4;
DE AltName: Full=Serotonin receptor 4;
GN Name=HTR4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5-HT4(A); 5-HT4(B); 5-HT4(C) AND
RP 5-HT4(D)).
RC TISSUE=Intestine;
RX PubMed=9603189; DOI=10.1046/j.1471-4159.1998.70062252.x;
RA Blondel O., Gastineau M., Dahmoune Y., Langlois M., Fischmeister R.;
RT "Cloning, expression, and pharmacology of four human 5-hydroxytryptamine 4
RT receptor isoforms produced by alternative splicing in the carboxyl
RT terminus.";
RL J. Neurochem. 70:2252-2261(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5-HT4(A) AND 5-HT4(B)).
RC TISSUE=Brain;
RA Van den Wyngaert I., Gommeren W., Jurzak M., Verhasselt P., Gordon R.,
RA Leysen J., Luyten W., Bender E.;
RT "Cloning and expression of 5-HT4 receptor species and splice variants.";
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5-HT4(A)).
RC TISSUE=Heart;
RX PubMed=9351641; DOI=10.1097/00001756-199710200-00002;
RA Claeysen S., Faye P., Sebben M., Lemaire S., Bockaert J., Dumuis A.;
RT "Cloning and expression of human 5-HT4S receptors. Effect of receptor
RT density on their coupling to adenylyl cyclase.";
RL NeuroReport 8:3189-3195(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5-HT4(B)).
RA Syversveen T., Bach T., Kvingedal A.M., Krobert K.A., Kaumann A.J.,
RA Levy F.O.;
RT "Molecular cloning of the human serotonin receptor 5-HT4(b) and
RT pharmacological comparison with the cloned human 5-HT4(a) receptor.";
RL Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5-HT4(E)).
RC TISSUE=Brain;
RX PubMed=10220570;
RA Claeysen S., Sebben M., Becamel C., Bockaert J., Dumuis A.;
RT "Novel brain-specific 5-HT4 receptor splice variants show marked
RT constitutive activity: role of the C-terminal intracellular domain.";
RL Mol. Pharmacol. 55:910-920(1999).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5-HT4(A); 5-HT4(B); 5-HT4(E) AND
RP 5-HT4(G)).
RC TISSUE=Brain, and Hippocampus;
RX PubMed=11750916; DOI=10.1016/s0028-3908(01)00154-x;
RA Vilaro M.T., Domenech T., Palacios J.M., Mengod G.;
RT "Cloning and characterization of a novel human 5-HT4 receptor variant that
RT lacks the alternatively spliced carboxy terminal exon. RT-PCR distribution
RT in human brain and periphery of multiple 5-HT4 receptor variants.";
RL Neuropharmacology 42:60-73(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5-HT4(E) AND 5-HT4(I)), AND TISSUE
RP SPECIFICITY.
RX PubMed=15118808; DOI=10.1007/s00210-004-0919-4;
RA Brattelid T., Kvingedal A.M., Krobert K.A., Andressen K.W., Bach T.,
RA Hystad M.E., Kaumann A.J., Levy F.O.;
RT "Cloning, pharmacological characterisation and tissue distribution of a
RT novel 5-HT4 receptor splice variant, 5-HT4(i).";
RL Naunyn Schmiedebergs Arch. Pharmacol. 369:616-628(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5-HT4C1).
RC TISSUE=Small intestine;
RA Reid A.M., Kelsell R.E., Houp J.A., Kelly F.M., Medhurst A.D., Cox H.M.,
RA Calver A.R.;
RT "Cloning, expression and characterisation of a novel rat 5-HT4 receptor
RT splice variant (5-HT4c1).";
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [10]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [11]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5-HT4(B)).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [12]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 10-388 (ISOFORM 5-HT4(F)).
RX PubMed=10646498; DOI=10.1046/j.1471-4159.2000.740478.x;
RA Bender E., Pindon A., van Oers I., Zhang Y.B., Gommeren W., Verhasselt P.,
RA Jurzak M., Leysen J., Luyten W.;
RT "Structure of the human serotonin 5-HT4 receptor gene and cloning of a
RT novel 5-HT4 splice variant.";
RL J. Neurochem. 74:478-489(2000).
RN [13]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 112-255.
RC TISSUE=Brain;
RX PubMed=7656980; DOI=10.1016/0014-5793(95)00828-w;
RA Ullmer C., Schmuck K., Kalkman H.O., Luebbert H.;
RT "Expression of serotonin receptor mRNAs in blood vessels.";
RL FEBS Lett. 370:215-221(1995).
RN [14]
RP INTERACTION WITH GRK5, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=19661922; DOI=10.1038/emboj.2009.215;
RA Barthet G., Carrat G., Cassier E., Barker B., Gaven F., Pillot M.,
RA Framery B., Pellissier L.P., Augier J., Kang D.S., Claeysen S., Reiter E.,
RA Baneres J.L., Benovic J.L., Marin P., Bockaert J., Dumuis A.;
RT "Beta-arrestin1 phosphorylation by GRK5 regulates G protein-independent 5-
RT HT4 receptor signalling.";
RL EMBO J. 28:2706-2718(2009).
RN [15]
RP VARIANT LEU-27.
RX PubMed=22197934; DOI=10.1038/ng.1027;
RA Rademakers R., Baker M., Nicholson A.M., Rutherford N.J., Finch N.,
RA Soto-Ortolaza A., Lash J., Wider C., Wojtas A., DeJesus-Hernandez M.,
RA Adamson J., Kouri N., Sundal C., Shuster E.A., Aasly J., MacKenzie J.,
RA Roeber S., Kretzschmar H.A., Boeve B.F., Knopman D.S., Petersen R.C.,
RA Cairns N.J., Ghetti B., Spina S., Garbern J., Tselis A.C., Uitti R.,
RA Das P., Van Gerpen J.A., Meschia J.F., Levy S., Broderick D.F.,
RA Graff-Radford N., Ross O.A., Miller B.B., Swerdlow R.H., Dickson D.W.,
RA Wszolek Z.K.;
RT "Mutations in the colony stimulating factor 1 receptor (CSF1R) gene cause
RT hereditary diffuse leukoencephalopathy with spheroids.";
RL Nat. Genet. 44:200-205(2012).
CC -!- FUNCTION: This is one of the several different receptors for 5-
CC hydroxytryptamine (serotonin), a biogenic hormone that functions as a
CC neurotransmitter, a hormone, and a mitogen. The activity of this
CC receptor is mediated by G proteins that stimulate adenylate cyclase.
CC -!- SUBUNIT: Isoform 5-HT4(A) interacts with MAGI2, MPP3, SLC9A3R1 and
CC SNX27 isoforms 1 and 2. Isoform 5-HT4(E) interacts with PATJ, NOS1 and
CC SEC23A. Isoform 5-HT4(A) forms a complex including SLC9A3R1 and EZR (By
CC similarity). Interacts (via C-terminus 330-346 AA) with GRK5; this
CC interaction is promoted by 5-HT (serotonin). {ECO:0000250,
CC ECO:0000269|PubMed:19661922}.
CC -!- INTERACTION:
CC Q13639; O15354: GPR37; NbExp=5; IntAct=EBI-6656425, EBI-15639515;
CC Q13639; O60269: GPRIN2; NbExp=2; IntAct=EBI-6656425, EBI-740397;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC Endosome. Note=Interaction with SNX27 mediates recruitment to early
CC endosomes, while interaction with SLC9A3R1 and EZR might target the
CC protein to specialized subcellular regions, such as microvilli.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=9;
CC Comment=Additional isoforms seem to exist.;
CC Name=5-HT4(B);
CC IsoId=Q13639-1; Sequence=Displayed;
CC Name=5-HT4(A); Synonyms=5-HT4S;
CC IsoId=Q13639-2; Sequence=VSP_001849;
CC Name=5-HT4(C);
CC IsoId=Q13639-3; Sequence=VSP_001848;
CC Name=5-HT4(D);
CC IsoId=Q13639-4; Sequence=VSP_001847;
CC Name=5-HT4(E); Synonyms=H5-HT4(g);
CC IsoId=Q13639-5; Sequence=VSP_001846;
CC Name=5-HT4(F);
CC IsoId=Q13639-6; Sequence=VSP_001845;
CC Name=5-HT4(G);
CC IsoId=Q13639-7; Sequence=VSP_001850;
CC Name=5-HT4(I);
CC IsoId=Q13639-8; Sequence=VSP_043316;
CC Name=5-HT4c1;
CC IsoId=Q13639-9; Sequence=VSP_047862;
CC -!- TISSUE SPECIFICITY: Isoform 5-HT4(A) is expressed in ileum, brain, and
CC atrium, but not in the ventricle. {ECO:0000269|PubMed:15118808}.
CC -!- MISCELLANEOUS: [Isoform 5-HT4(E)]: Mainly expressed in atria and
CC cardiac ventricle. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 5-HT4(I)]: Expressed in all cardiovascular
CC tissues analyzed. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; Y08756; CAA70002.1; -; mRNA.
DR EMBL; Y12505; CAA73107.1; -; mRNA.
DR EMBL; Y12506; CAA73108.1; -; mRNA.
DR EMBL; Y12507; CAA73109.1; -; mRNA.
DR EMBL; Y10437; CAA71462.1; -; mRNA.
DR EMBL; Y13584; CAA73911.1; -; mRNA.
DR EMBL; Y09586; CAA70774.1; -; mRNA.
DR EMBL; AJ131724; CAC20411.1; -; mRNA.
DR EMBL; AJ011371; CAA09600.1; -; mRNA.
DR EMBL; AJ278979; CAC22248.1; -; mRNA.
DR EMBL; AJ278980; CAC22249.1; -; mRNA.
DR EMBL; AJ278981; CAC22250.1; -; mRNA.
DR EMBL; AJ278982; CAC22251.1; -; mRNA.
DR EMBL; AJ131725; CAC79533.1; -; mRNA.
DR EMBL; AJ131726; CAC79538.1; -; mRNA.
DR EMBL; AJ633645; CAG17627.1; -; mRNA.
DR EMBL; AM712912; CAN84676.1; -; mRNA.
DR EMBL; AC008627; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC011390; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC091971; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC114939; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471062; EAW61800.1; -; Genomic_DNA.
DR EMBL; CH471062; EAW61801.1; -; Genomic_DNA.
DR EMBL; CH471062; EAW61804.1; -; Genomic_DNA.
DR EMBL; BC074755; AAH74755.1; -; mRNA.
DR EMBL; BC095497; AAH95497.1; -; mRNA.
DR EMBL; AJ243213; CAB71316.1; -; Genomic_DNA.
DR EMBL; Z48150; CAA88167.1; -; mRNA.
DR CCDS; CCDS34270.1; -. [Q13639-2]
DR CCDS; CCDS34271.1; -. [Q13639-5]
DR CCDS; CCDS34272.1; -. [Q13639-8]
DR CCDS; CCDS34273.2; -. [Q13639-3]
DR CCDS; CCDS4291.1; -. [Q13639-1]
DR CCDS; CCDS75353.1; -. [Q13639-9]
DR PIR; S66493; S66493.
DR RefSeq; NP_000861.1; NM_000870.6. [Q13639-1]
DR RefSeq; NP_001035259.1; NM_001040169.2. [Q13639-2]
DR RefSeq; NP_001035262.2; NM_001040172.2. [Q13639-3]
DR RefSeq; NP_001035263.1; NM_001040173.2. [Q13639-8]
DR RefSeq; NP_001273339.1; NM_001286410.1. [Q13639-9]
DR RefSeq; NP_955525.1; NM_199453.3. [Q13639-5]
DR PDB; 5EM9; X-ray; 1.60 A; B=264-268.
DR PDBsum; 5EM9; -.
DR AlphaFoldDB; Q13639; -.
DR SMR; Q13639; -.
DR BioGRID; 109592; 17.
DR IntAct; Q13639; 17.
DR MINT; Q13639; -.
DR STRING; 9606.ENSP00000353915; -.
DR BindingDB; Q13639; -.
DR ChEMBL; CHEMBL1875; -.
DR DrugBank; DB01239; Chlorprothixene.
DR DrugBank; DB08810; Cinitapride.
DR DrugBank; DB00604; Cisapride.
DR DrugBank; DB11273; Dihydroergocornine.
DR DrugBank; DB13345; Dihydroergocristine.
DR DrugBank; DB00320; Dihydroergotamine.
DR DrugBank; DB01049; Ergoloid mesylate.
DR DrugBank; DB12141; Gilteritinib.
DR DrugBank; DB01233; Metoclopramide.
DR DrugBank; DB05542; Naronapride.
DR DrugBank; DB00904; Ondansetron.
DR DrugBank; DB00715; Paroxetine.
DR DrugBank; DB04873; Piboserod.
DR DrugBank; DB06480; Prucalopride.
DR DrugBank; DB04917; Renzapride.
DR DrugBank; DB09304; Setiptiline.
DR DrugBank; DB05905; TD-2749.
DR DrugBank; DB01079; Tegaserod.
DR DrugBank; DB13025; Tiapride.
DR DrugBank; DB06422; Ticalopride.
DR DrugBank; DB12702; Velusetrag.
DR DrugCentral; Q13639; -.
DR GuidetoPHARMACOLOGY; 9; -.
DR TCDB; 9.A.14.3.18; the g-protein-coupled receptor (gpcr) family.
DR GlyGen; Q13639; 1 site.
DR iPTMnet; Q13639; -.
DR PhosphoSitePlus; Q13639; -.
DR SwissPalm; Q13639; -.
DR BioMuta; HTR4; -.
DR DMDM; 12644029; -.
DR PeptideAtlas; Q13639; -.
DR PRIDE; Q13639; -.
DR Antibodypedia; 15936; 453 antibodies from 33 providers.
DR DNASU; 3360; -.
DR Ensembl; ENST00000360693.7; ENSP00000353915.3; ENSG00000164270.18. [Q13639-8]
DR Ensembl; ENST00000362016.6; ENSP00000355037.3; ENSG00000164270.18. [Q13639-8]
DR Ensembl; ENST00000377888.8; ENSP00000367120.4; ENSG00000164270.18. [Q13639-1]
DR Ensembl; ENST00000517929.5; ENSP00000435904.1; ENSG00000164270.18. [Q13639-3]
DR Ensembl; ENST00000520514.5; ENSP00000427913.1; ENSG00000164270.18. [Q13639-9]
DR Ensembl; ENST00000521530.5; ENSP00000428320.1; ENSG00000164270.18. [Q13639-2]
DR Ensembl; ENST00000521735.5; ENSP00000430979.1; ENSG00000164270.18. [Q13639-5]
DR Ensembl; ENST00000522588.5; ENSP00000430874.1; ENSG00000164270.18. [Q13639-5]
DR Ensembl; ENST00000631296.1; ENSP00000485747.1; ENSG00000164270.18. [Q13639-1]
DR GeneID; 3360; -.
DR KEGG; hsa:3360; -.
DR MANE-Select; ENST00000377888.8; ENSP00000367120.4; NM_000870.7; NP_000861.1.
DR UCSC; uc003lpn.5; human. [Q13639-1]
DR CTD; 3360; -.
DR DisGeNET; 3360; -.
DR GeneCards; HTR4; -.
DR HGNC; HGNC:5299; HTR4.
DR HPA; ENSG00000164270; Group enriched (brain, heart muscle, intestine).
DR MIM; 602164; gene.
DR neXtProt; NX_Q13639; -.
DR OpenTargets; ENSG00000164270; -.
DR PharmGKB; PA29557; -.
DR VEuPathDB; HostDB:ENSG00000164270; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000155983; -.
DR HOGENOM; CLU_009579_11_0_1; -.
DR InParanoid; Q13639; -.
DR OMA; AHQIRIL; -.
DR OrthoDB; 750855at2759; -.
DR PhylomeDB; Q13639; -.
DR TreeFam; TF316350; -.
DR PathwayCommons; Q13639; -.
DR Reactome; R-HSA-390666; Serotonin receptors.
DR Reactome; R-HSA-418555; G alpha (s) signalling events.
DR Reactome; R-HSA-9660821; ADORA2B mediated anti-inflammatory cytokines production.
DR SignaLink; Q13639; -.
DR SIGNOR; Q13639; -.
DR BioGRID-ORCS; 3360; 10 hits in 1069 CRISPR screens.
DR ChiTaRS; HTR4; human.
DR GeneWiki; 5-HT4_receptor; -.
DR GenomeRNAi; 3360; -.
DR Pharos; Q13639; Tclin.
DR PRO; PR:Q13639; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q13639; protein.
DR Bgee; ENSG00000164270; Expressed in type B pancreatic cell and 179 other tissues.
DR ExpressionAtlas; Q13639; baseline and differential.
DR Genevisible; Q13639; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IDA:MGI.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0051378; F:serotonin binding; IBA:GO_Central.
DR GO; GO:0007198; P:adenylate cyclase-inhibiting serotonin receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR GO; GO:0032098; P:regulation of appetite; IEA:InterPro.
DR InterPro; IPR001520; 5HT4_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01059; 5HT4RECEPTR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Disulfide bond;
KW Endosome; G-protein coupled receptor; Glycoprotein; Lipoprotein; Membrane;
KW Palmitate; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..388
FT /note="5-hydroxytryptamine receptor 4"
FT /id="PRO_0000068965"
FT TOPO_DOM 1..19
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 20..40
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 41..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 59..79
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 80..93
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 94..116
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 117..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 138..158
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 159..192
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 193..213
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 214..260
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 261..281
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 282..294
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 295..315
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 316..388
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT LIPID 329
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT CARBOHYD 7
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 93..184
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 169
FT /note="L -> LERSLNQGLGQDFHA (in isoform 5-HT4(F))"
FT /evidence="ECO:0000305"
FT /id="VSP_001845"
FT VAR_SEQ 359..388
FT /note="RDAVECGGQWESQCHPPATSPLVAAQPSDT -> SSGTETDRRNFGIRKRRL
FT TKPS (in isoform 5-HT4(D))"
FT /evidence="ECO:0000305"
FT /id="VSP_001847"
FT VAR_SEQ 359..388
FT /note="RDAVECGGQWESQCHPPATSPLVAAQPSDT -> SGCSPVSSFLLLFCNRPV
FT PV (in isoform 5-HT4(E))"
FT /evidence="ECO:0000305"
FT /id="VSP_001846"
FT VAR_SEQ 359..388
FT /note="RDAVECGGQWESQCHPPATSPLVAAQPSDT -> SSGTETDRKKLWNKEEKI
FT DQTIQMPKRKRKKKASLSYEDLILLGRKSCFREGK (in isoform 5-HT4c1)"
FT /evidence="ECO:0000303|Ref.8"
FT /id="VSP_047862"
FT VAR_SEQ 359
FT /note="R -> RTDFLFDRDILARYWTKPARAGPFSGTLSIRCLTARKPVLG (in
FT isoform 5-HT4(I))"
FT /evidence="ECO:0000305"
FT /id="VSP_043316"
FT VAR_SEQ 360..388
FT /note="DAVECGGQWESQCHPPATSPLVAAQPSDT -> YTVLHRGHHQELEKLPIHN
FT DPESLESCF (in isoform 5-HT4(A))"
FT /evidence="ECO:0000305"
FT /id="VSP_001849"
FT VAR_SEQ 360..388
FT /note="DAVECGGQWESQCHPPATSPLVAAQPSDT -> F (in isoform 5-
FT HT4(C))"
FT /evidence="ECO:0000305"
FT /id="VSP_001848"
FT VAR_SEQ 360..388
FT /note="Missing (in isoform 5-HT4(G))"
FT /evidence="ECO:0000305"
FT /id="VSP_001850"
FT VARIANT 27
FT /note="S -> L (in a patient with diffuse
FT leukoencephalopathy with spheroids; unknown pathological
FT significance; dbSNP:rs199508638)"
FT /evidence="ECO:0000269|PubMed:22197934"
FT /id="VAR_067412"
FT VARIANT 372
FT /note="C -> Y (in dbSNP:rs34826744)"
FT /id="VAR_049364"
FT STRAND 264..268
FT /evidence="ECO:0007829|PDB:5EM9"
SQ SEQUENCE 388 AA; 43761 MW; 7FCFEC60E7BDF560 CRC64;
MDKLDANVSS EEGFGSVEKV VLLTFLSTVI LMAILGNLLV MVAVCWDRQL RKIKTNYFIV
SLAFADLLVS VLVMPFGAIE LVQDIWIYGE VFCLVRTSLD VLLTTASIFH LCCISLDRYY
AICCQPLVYR NKMTPLRIAL MLGGCWVIPT FISFLPIMQG WNNIGIIDLI EKRKFNQNSN
STYCVFMVNK PYAITCSVVA FYIPFLLMVL AYYRIYVTAK EHAHQIQMLQ RAGASSESRP
QSADQHSTHR MRTETKAAKT LCIIMGCFCL CWAPFFVTNI VDPFIDYTVP GQVWTAFLWL
GYINSGLNPF LYAFLNKSFR RAFLIILCCD DERYRRPSIL GQTVPCSTTT INGSTHVLRD
AVECGGQWES QCHPPATSPL VAAQPSDT
