5HT5A_MOUSE
ID 5HT5A_MOUSE Reviewed; 357 AA.
AC P30966; Q3URB0;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=5-hydroxytryptamine receptor 5A;
DE Short=5-HT-5;
DE Short=5-HT-5A;
DE Short=5-HT5A;
DE AltName: Full=Serotonin receptor 5A;
GN Name=Htr5a; Synonyms=5ht5a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Brain;
RX PubMed=1464308; DOI=10.1002/j.1460-2075.1992.tb05583.x;
RA Plassat J.-L., Boschert U., Amlaiky N., Hen R.;
RT "The mouse 5HT5 receptor reveals a remarkable heterogeneity within the
RT 5HT1D receptor family.";
RL EMBO J. 11:4779-4786(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Hippocampus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP CHARACTERIZATION.
RC TISSUE=Brain;
RX PubMed=8450829;
RA Matthes H., Boschert U., Amlaiky N., Grailhe R., Plassat J.-L.,
RA Muscatelli F., Mattei M.-G., Hen R.;
RT "Mouse 5-hydroxytryptamine5A and 5-hydroxytryptamine5B receptors define a
RT new family of serotonin receptors: cloning, functional expression, and
RT chromosomal localization.";
RL Mol. Pharmacol. 43:313-319(1993).
CC -!- FUNCTION: This is one of the several different receptors for 5-
CC hydroxytryptamine (serotonin), a biogenic hormone that functions as a
CC neurotransmitter, a hormone, and a mitogen. The activity of this
CC receptor is mediated by G proteins.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in the central nervous
CC system; in the cerebral cortex, hippocampus, habenula, olfactory bulb
CC and granular layer of the cerebellum.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; Z18278; CAA79155.1; -; Genomic_DNA.
DR EMBL; AK141636; BAE24778.1; -; mRNA.
DR EMBL; CH466524; EDL37224.1; -; Genomic_DNA.
DR EMBL; BC115911; AAI15912.1; -; mRNA.
DR EMBL; BC116654; AAI16655.1; -; mRNA.
DR CCDS; CCDS19141.1; -.
DR PIR; S28058; S28058.
DR RefSeq; NP_032340.2; NM_008314.2.
DR AlphaFoldDB; P30966; -.
DR SMR; P30966; -.
DR STRING; 10090.ENSMUSP00000038884; -.
DR BindingDB; P30966; -.
DR ChEMBL; CHEMBL3597; -.
DR DrugCentral; P30966; -.
DR GuidetoPHARMACOLOGY; 10; -.
DR GlyGen; P30966; 2 sites.
DR PhosphoSitePlus; P30966; -.
DR PaxDb; P30966; -.
DR PRIDE; P30966; -.
DR ProteomicsDB; 296452; -.
DR Antibodypedia; 18848; 357 antibodies from 30 providers.
DR DNASU; 15563; -.
DR Ensembl; ENSMUST00000036227; ENSMUSP00000038884; ENSMUSG00000039106.
DR GeneID; 15563; -.
DR KEGG; mmu:15563; -.
DR UCSC; uc008wtt.1; mouse.
DR CTD; 3361; -.
DR MGI; MGI:96283; Htr5a.
DR VEuPathDB; HostDB:ENSMUSG00000039106; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01010000222287; -.
DR HOGENOM; CLU_009579_11_6_1; -.
DR InParanoid; P30966; -.
DR OMA; RRISNIM; -.
DR OrthoDB; 817833at2759; -.
DR PhylomeDB; P30966; -.
DR TreeFam; TF316350; -.
DR Reactome; R-MMU-390666; Serotonin receptors.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR BioGRID-ORCS; 15563; 3 hits in 72 CRISPR screens.
DR PRO; PR:P30966; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; P30966; protein.
DR Bgee; ENSMUSG00000039106; Expressed in secondary oocyte and 41 other tissues.
DR Genevisible; P30966; MM.
DR GO; GO:0030425; C:dendrite; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099060; C:integral component of postsynaptic specialization membrane; ISO:MGI.
DR GO; GO:0043204; C:perikaryon; ISO:MGI.
DR GO; GO:0005791; C:rough endoplasmic reticulum; ISO:MGI.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; ISO:MGI.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0051378; F:serotonin binding; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IDA:MGI.
DR GO; GO:0007198; P:adenylate cyclase-inhibiting serotonin receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; ISO:MGI.
DR GO; GO:0021766; P:hippocampus development; IEA:Ensembl.
DR GO; GO:0032355; P:response to estradiol; IEA:Ensembl.
DR InterPro; IPR001397; 5HT5A_rcpt.
DR InterPro; IPR002231; 5HT_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR PANTHER; PTHR24247:SF108; PTHR24247:SF108; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00518; 5HT5ARECEPTR.
DR PRINTS; PR01101; 5HTRECEPTOR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..357
FT /note="5-hydroxytryptamine receptor 5A"
FT /id="PRO_0000068970"
FT TOPO_DOM 1..40
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 41..63
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 64..78
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 79..99
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 100..115
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 116..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 138..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 159..181
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 182..198
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 199..219
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 220..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 283..303
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 304..320
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 321..341
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 342..357
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 21
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 120..192
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 214..215
FT /note="CV -> WL (in Ref. 1; CAA79155)"
FT /evidence="ECO:0000305"
FT CONFLICT 261
FT /note="V -> A (in Ref. 1; CAA79155)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 357 AA; 40736 MW; C0F7314A3AB9F75F CRC64;
MDLPVNLTSF SLSTPSSLEP NRSLDTEVLR PSRPFLSAFR VLVLTLLGFL AAATFTWNLL
VLATILKVRT FHRVPHNLVA SMAISDVLVA VLVMPLSLVH ELSGRRWQLG RRLCQLWIAC
DVLCCTASIW NVTAIALDRY WSITRHLEYT LRTRKRVSNV MILLTWALST VISLAPLLFG
WGETYSEPSE ECQVSREPSY TVFSTVGAFY LPLCVVLFVY WKIYRAAKFR MGSRKTNSVS
PVPEAVEVKN ATQHPQMVFT VRHATVTFQT EGDTWREQKE QRAALMVGIL IGVFVLCWFP
FFVTELISPL CSWDVPAIWK SIFLWLGYSN SFFNPLIYTA FNRSYSSAFK VFFSKQQ
