ID   LPXK_CAMJE              Reviewed;         308 AA.
AC   Q9PPA9; Q0PA79;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Tetraacyldisaccharide 4'-kinase {ECO:0000255|HAMAP-Rule:MF_00409};
DE            EC=2.7.1.130 {ECO:0000255|HAMAP-Rule:MF_00409};
DE   AltName: Full=Lipid A 4'-kinase {ECO:0000255|HAMAP-Rule:MF_00409};
GN   Name=lpxK {ECO:0000255|HAMAP-Rule:MF_00409}; OrderedLocusNames=Cj0811;
OS   Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC
OS   11168).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=192222;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700819 / NCTC 11168;
RX   PubMed=10688204; DOI=10.1038/35001088;
RA   Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M.,
RA   Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S.,
RA   Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A.,
RA   Rajandream M.A., Rutherford K.M., van Vliet A.H.M., Whitehead S.,
RA   Barrell B.G.;
RT   "The genome sequence of the food-borne pathogen Campylobacter jejuni
RT   reveals hypervariable sequences.";
RL   Nature 403:665-668(2000).
CC   -!- FUNCTION: Transfers the gamma-phosphate of ATP to the 4'-position of a
CC       tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form
CC       tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).
CC       {ECO:0000255|HAMAP-Rule:MF_00409}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + {2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-beta-D-
CC         glucosaminyl}-(1->6)-{2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-
CC         D-glucosaminyl phosphate} = ADP + {2-N,3-O-bis[(3R)-3-
CC         hydroxytetradecanoyl]-4-O-phospho-beta-D-glucosaminyl}-(1->6)-{2-N,3-
CC         O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl phosphate}.;
CC         EC=2.7.1.130; Evidence={ECO:0000255|HAMAP-Rule:MF_00409};
CC   -!- PATHWAY: Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A)
CC       from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-
CC       acetyl-alpha-D-glucosamine: step 6/6. {ECO:0000255|HAMAP-
CC       Rule:MF_00409}.
CC   -!- SIMILARITY: Belongs to the LpxK family. {ECO:0000255|HAMAP-
CC       Rule:MF_00409}.
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DR   EMBL; AL111168; CAL34939.1; -; Genomic_DNA.
DR   PIR; C81353; C81353.
DR   RefSeq; WP_002852582.1; NC_002163.1.
DR   RefSeq; YP_002344218.1; NC_002163.1.
DR   AlphaFoldDB; Q9PPA9; -.
DR   SMR; Q9PPA9; -.
DR   STRING; 192222.Cj0811; -.
DR   PaxDb; Q9PPA9; -.
DR   PRIDE; Q9PPA9; -.
DR   EnsemblBacteria; CAL34939; CAL34939; Cj0811.
DR   GeneID; 905114; -.
DR   KEGG; cje:Cj0811; -.
DR   PATRIC; fig|192222.6.peg.799; -.
DR   eggNOG; COG1663; Bacteria.
DR   HOGENOM; CLU_038816_1_0_7; -.
DR   OMA; YYPFCLP; -.
DR   UniPathway; UPA00359; UER00482.
DR   Proteomes; UP000000799; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009029; F:tetraacyldisaccharide 4'-kinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   HAMAP; MF_00409; LpxK; 1.
DR   InterPro; IPR003758; LpxK.
DR   PANTHER; PTHR42724; PTHR42724; 2.
DR   Pfam; PF02606; LpxK; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Lipid A biosynthesis; Lipid biosynthesis;
KW   Lipid metabolism; Nucleotide-binding; Reference proteome; Transferase.
FT   CHAIN           1..308
FT                   /note="Tetraacyldisaccharide 4'-kinase"
FT                   /id="PRO_0000190916"
FT   BINDING         63..70
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00409"
SQ   SEQUENCE   308 AA;  36228 MW;  012CE5FD020D3C71 CRC64;
     MNEEKNYELW LDNYFFKPNF WQKCLAFILL PLSVLYAFFA ILNTFFRKKI VFKKPVISVG
     NLSFGGNGKT PLCKAIAREF DGVFIVLRGY KRKSKGLFVV KNQNEILCTL TQSGDEAMEY
     AFEENIKGVI VSEDRVKGIE KAFELGAKIV VLDDAFSKFH IKKFDILLES KIKPYFNFTL
     PSGAYRLPKF YEKRADFIAL EGRDFVRYSF VKENPKAVLV TAIAKPFRLY EHFIKARACY
     FFKDHYEFKK EELENLLKKH NCDTLMLTFK DFVKVKDFGF KCQIIELNIE LKDSLREKIK
     TYIKEFEQ