LRC14_MOUSE
ID LRC14_MOUSE Reviewed; 493 AA.
AC Q8VC16; Q6A0E7; Q80WK3;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Leucine-rich repeat-containing protein 14 {ECO:0000250|UniProtKB:Q15048};
GN Name=Lrrc14 {ECO:0000312|MGI:MGI:2445060};
GN Synonyms=Kiaa0014 {ECO:0000303|PubMed:15368895};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RA Uwanogho D.A., Mellodew K., Molloy G., Galloway D., Starling B.B.,
RA Crossland N., Price J.;
RT "A novel program of gene expression driven by Notch, is associated with
RT commitment in neural stem cell lines.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Brain, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=27426725; DOI=10.1016/j.yexcr.2016.07.011;
RA Wu C., Yang Y., Ou J., Zhu L., Zhao W., Cui J.;
RT "LRRC14 attenuates Toll-like receptor-mediated NF-kappa-B signaling through
RT disruption of IKK complex.";
RL Exp. Cell Res. 347:65-73(2016).
CC -!- FUNCTION: Negatively regulates Toll-like receptor-mediated NF-kappa-B
CC signaling by disrupting IKK core complex formation through interaction
CC with IKBKB. {ECO:0000250|UniProtKB:Q15048}.
CC -!- SUBUNIT: Interacts with IKBKB; disrupts IKBKB-IKBKG interaction
CC preventing I-kappa-B-kinase (IKK) core complex formation and leading to
CC a decrease of IKBKB phosphorylation and NF-kappaB activation. Interacts
CC with CHUK. {ECO:0000250|UniProtKB:Q15048}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q15048}.
CC -!- SIMILARITY: Belongs to the PRAME family. LRRC14 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD32149.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AY239226; AAP23937.1; -; mRNA.
DR EMBL; AK172871; BAD32149.1; ALT_FRAME; Transcribed_RNA.
DR EMBL; AK036278; BAC29370.1; -; mRNA.
DR EMBL; AK159836; BAE35414.1; -; mRNA.
DR EMBL; CH466545; EDL29611.1; -; Genomic_DNA.
DR EMBL; CH466545; EDL29614.1; -; Genomic_DNA.
DR EMBL; BC022126; AAH22126.1; -; mRNA.
DR EMBL; BC138217; AAI38218.1; -; mRNA.
DR CCDS; CCDS27589.1; -.
DR RefSeq; NP_663446.1; NM_145471.2.
DR RefSeq; XP_006520848.1; XM_006520785.3.
DR RefSeq; XP_006520849.1; XM_006520786.3.
DR RefSeq; XP_006520850.1; XM_006520787.2.
DR RefSeq; XP_006520852.1; XM_006520789.3.
DR RefSeq; XP_011243881.1; XM_011245579.2.
DR RefSeq; XP_017172047.1; XM_017316558.1.
DR RefSeq; XP_017172048.1; XM_017316559.1.
DR AlphaFoldDB; Q8VC16; -.
DR STRING; 10090.ENSMUSP00000121982; -.
DR iPTMnet; Q8VC16; -.
DR PhosphoSitePlus; Q8VC16; -.
DR EPD; Q8VC16; -.
DR MaxQB; Q8VC16; -.
DR PaxDb; Q8VC16; -.
DR PeptideAtlas; Q8VC16; -.
DR PRIDE; Q8VC16; -.
DR ProteomicsDB; 290149; -.
DR Antibodypedia; 14965; 59 antibodies from 16 providers.
DR DNASU; 223664; -.
DR Ensembl; ENSMUST00000036423; ENSMUSP00000049466; ENSMUSG00000033728.
DR Ensembl; ENSMUST00000127208; ENSMUSP00000114921; ENSMUSG00000033728.
DR Ensembl; ENSMUST00000137649; ENSMUSP00000121982; ENSMUSG00000033728.
DR Ensembl; ENSMUST00000155735; ENSMUSP00000115446; ENSMUSG00000033728.
DR GeneID; 223664; -.
DR KEGG; mmu:223664; -.
DR UCSC; uc007wlx.2; mouse.
DR CTD; 9684; -.
DR MGI; MGI:2445060; Lrrc14.
DR VEuPathDB; HostDB:ENSMUSG00000033728; -.
DR eggNOG; ENOG502QWSJ; Eukaryota.
DR GeneTree; ENSGT01030000234531; -.
DR HOGENOM; CLU_039635_0_1_1; -.
DR InParanoid; Q8VC16; -.
DR OMA; VTECELM; -.
DR OrthoDB; 563553at2759; -.
DR PhylomeDB; Q8VC16; -.
DR TreeFam; TF332708; -.
DR BioGRID-ORCS; 223664; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Lrrc14; mouse.
DR PRO; PR:Q8VC16; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8VC16; protein.
DR Bgee; ENSMUSG00000033728; Expressed in embryonic brain and 252 other tissues.
DR ExpressionAtlas; Q8VC16; baseline and differential.
DR Genevisible; Q8VC16; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0019900; F:kinase binding; ISO:MGI.
DR GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
DR GO; GO:0034122; P:negative regulation of toll-like receptor signaling pathway; ISS:UniProtKB.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR026292; LRRC14.
DR PANTHER; PTHR14224:SF9; PTHR14224:SF9; 1.
DR Pfam; PF13516; LRR_6; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..493
FT /note="Leucine-rich repeat-containing protein 14"
FT /id="PRO_0000343681"
FT REPEAT 111..146
FT /note="LRR 1; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 194..218
FT /note="LRR 2; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 219..246
FT /note="LRR 3; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 247..282
FT /note="LRR 4; degenerate"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 283..307
FT /note="LRR 5"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 308..339
FT /note="LRR 6"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 340..360
FT /note="LRR 7"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 364..391
FT /note="LRR 8"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT REPEAT 392..416
FT /note="LRR 9"
FT /evidence="ECO:0000250|UniProtKB:Q3UWY1"
FT CONFLICT 49..50
FT /note="EL -> DV (in Ref. 1; AAP23937)"
FT /evidence="ECO:0000305"
FT CONFLICT 155
FT /note="T -> TA (in Ref. 1; AAP23937)"
FT /evidence="ECO:0000305"
FT CONFLICT 160..161
FT /note="LS -> CP (in Ref. 1; AAP23937)"
FT /evidence="ECO:0000305"
FT CONFLICT 255
FT /note="L -> V (in Ref. 1; AAP23937)"
FT /evidence="ECO:0000305"
FT CONFLICT 327
FT /note="R -> C (in Ref. 2; BAD32149)"
FT /evidence="ECO:0000305"
FT CONFLICT 363..364
FT /note="VA -> SS (in Ref. 1; AAP23937)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 493 AA; 54969 MW; DD516098BA9DBE89 CRC64;
MHTLVFLSTR QVLQCQPAAC QALPLLPREL FPLLFKVAFM DKKTLVLREL VHTWPFPLLS
FQQLLQECAH CSRALLQERL STESMQAVIL GLTARIHTQE TEAGTQPLCR KHALRVLDMT
GLLDDGVEQD PETMSMWDCT AAVARTCIAQ QQGGTAEPGL SPVPVEIRVD LRVNRASYTF
LREALQSSVA SPLRLCCRDL RAEDLPMRNT VALLQLLDAG CLRRIDLRFN NLGLRGLSVI
IPHVARFQHL ASLRLHYVHG DSRQPSVDGE DNFRYFLAQM GRFMCLRELS MGSSLLSGRL
DQLLSTLQRP LESLELAFCA LLPEDLRFLA QSSHAAHLKK LDLSGNDLSG NQLTPFQGLL
QAVATTLLHL ELTECQLADA QLLATLPTLT RCASLRYLGL YGNPLSMAGL KELLRDSVVQ
AELRTVVHPF PVDCYEGLPW PPPASVLLEA SINEEKFARV EAELHQLLLA SGRAHVLWTT
DIYGRLAADY FSL
