LRC34_MOUSE
ID LRC34_MOUSE Reviewed; 415 AA.
AC Q9DAM1; A0AUN5; Q14B04;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Leucine-rich repeat-containing protein 34;
GN Name=Lrrc34 {ECO:0000312|MGI:MGI:1919077};
GN Synonyms=Spata34 {ECO:0000303|PubMed:24435972};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=BALB/cJ {ECO:0000312|EMBL:AEI83866.1};
RC TISSUE=Testis {ECO:0000312|EMBL:AEI83866.1};
RX PubMed=24435972; DOI=10.1007/s11033-013-3017-1;
RA Chen T., Jiang Z., Xu W., Wang Y., Li Y., Wan Y., Yuan W., Mo X., Wu X.,
RA Deng Y., Fan X., Nie D.;
RT "Expression and identification of a novel gene Spata34 in mouse
RT spermatogenic cells.";
RL Mol. Biol. Rep. 41:1683-1691(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP FUNCTION, INTERACTION WITH NPM1 AND NCL, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=24991885; DOI=10.1089/scd.2013.0470;
RA Luehrig S., Siamishi I., Tesmer-Wolf M., Zechner U., Engel W., Nolte J.;
RT "Lrrc34, a novel nucleolar protein, interacts with npm1 and ncl and has an
RT impact on pluripotent stem cells.";
RL Stem Cells Dev. 23:2862-2874(2014).
CC -!- FUNCTION: Highly expressed in stem cells where it may be involved in
CC regulation of pluripotency. In embryonic stem cells (ESCs), important
CC for normal expression of the pluripotency regulators POU5F1/OCT4 and
CC KLF4. Also important for expression of the ectodermal marker gene NES
CC and the endodermal marker gene GATA4. Promotes stem cell proliferation
CC in vitro. {ECO:0000269|PubMed:24991885}.
CC -!- SUBUNIT: Interacts with NPM1 and NCL. {ECO:0000269|PubMed:24991885}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24435972,
CC ECO:0000269|PubMed:24991885}. Nucleus, nucleolus
CC {ECO:0000269|PubMed:24991885}. Cytoplasm {ECO:0000269|PubMed:24435972,
CC ECO:0000269|PubMed:24991885}. Note=As stem cells differentiate,
CC translocates from the nucleolus to the nucleus and then to the
CC cytoplasm. Colocalizes with NPM1 and NCL in the nucleolus.
CC {ECO:0000269|PubMed:24991885}.
CC -!- TISSUE SPECIFICITY: Expressed in testis where it specifically localizes
CC to germ cells (at protein level) (PubMed:24435972, PubMed:24991885).
CC Not detected in other tissues tested (at protein level)
CC (PubMed:24435972, PubMed:24991885). Expressed in pluripotent embryonic
CC stem cells and multipotent adult germline stem cells (PubMed:24991885).
CC {ECO:0000269|PubMed:24435972, ECO:0000269|PubMed:24991885}.
CC -!- DEVELOPMENTAL STAGE: Detected in unfertilized oocytes; expression
CC continues in preimplantation embryos though to the blastula stage (at
CC protein level) (PubMed:24991885). In testis, detected in very early
CC spermatogonial germ cells with expression continuing through to the
CC elongated spermatid stage (at protein level) (PubMed:24435972,
CC PubMed:24991885). Weakly expressed in spermatozoa (at protein level)
CC (PubMed:24435972). Not detected in spermatozoa (at protein level)
CC (PubMed:24991885). {ECO:0000269|PubMed:24991885}.
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DR EMBL; JF934738; AEI83866.1; -; mRNA.
DR EMBL; AK005720; BAB24205.1; -; mRNA.
DR EMBL; CH466530; EDL34963.1; -; Genomic_DNA.
DR EMBL; BC100519; AAI00520.1; -; mRNA.
DR EMBL; BC116419; AAI16420.1; -; mRNA.
DR EMBL; BC116420; AAI16421.1; -; mRNA.
DR CCDS; CCDS38405.1; -.
DR RefSeq; NP_082217.1; NM_027941.1.
DR AlphaFoldDB; Q9DAM1; -.
DR SMR; Q9DAM1; -.
DR STRING; 10090.ENSMUSP00000029252; -.
DR iPTMnet; Q9DAM1; -.
DR PhosphoSitePlus; Q9DAM1; -.
DR MaxQB; Q9DAM1; -.
DR PaxDb; Q9DAM1; -.
DR PRIDE; Q9DAM1; -.
DR ProteomicsDB; 252505; -.
DR Antibodypedia; 50951; 81 antibodies from 19 providers.
DR Ensembl; ENSMUST00000029252; ENSMUSP00000029252; ENSMUSG00000027702.
DR GeneID; 71827; -.
DR KEGG; mmu:71827; -.
DR UCSC; uc008ovb.1; mouse.
DR CTD; 151827; -.
DR MGI; MGI:1919077; Lrrc34.
DR VEuPathDB; HostDB:ENSMUSG00000027702; -.
DR eggNOG; KOG4308; Eukaryota.
DR GeneTree; ENSGT00940000156456; -.
DR HOGENOM; CLU_017147_1_0_1; -.
DR InParanoid; Q9DAM1; -.
DR OMA; IKNCGMK; -.
DR OrthoDB; 1131848at2759; -.
DR PhylomeDB; Q9DAM1; -.
DR TreeFam; TF329653; -.
DR BioGRID-ORCS; 71827; 3 hits in 73 CRISPR screens.
DR PRO; PR:Q9DAM1; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q9DAM1; protein.
DR Bgee; ENSMUSG00000027702; Expressed in spermatid and 80 other tissues.
DR Genevisible; Q9DAM1; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13516; LRR_6; 6.
PE 1: Evidence at protein level;
KW Cytoplasm; Differentiation; Leucine-rich repeat; Nucleus;
KW Reference proteome; Repeat.
FT CHAIN 1..415
FT /note="Leucine-rich repeat-containing protein 34"
FT /id="PRO_0000228670"
FT REPEAT 246..272
FT /note="LRR 1"
FT REPEAT 274..296
FT /note="LRR 2"
SQ SEQUENCE 415 AA; 46635 MW; 347E2FF5F25EE237 CRC64;
MEKLQAQYCY LCSENIRKTN PFILNILQKL DEEIEKRPKE KFTVNIAGNN RLDSGQRITG
EDFWLLSKTL RNQPCISGVD VRYNLIGDVG AFYAAKLLQK QPSITYLNLM FNDIGPEGGE
LIAKALHKNK TLKYLRMTGN KIENTGGMLF AAMLQMNSSL EKLDLGDCDL GLQCVIAFST
VLTQNQAIKG INLNRPILYG EQEESTVHIG HMLKENHVLV ELHMCKHGMK NYGLQQLCNA
LYLNSSLRYL DVSCNKITRD GMVFLADVLK SNTTLEVLDL SFNRIETAGA KYLSETLTSH
NRSLKALSVV SNKIEGEGLV ALSQSMKTNL VLSNIYIWGN KFDEDTCVAY SDLIKSGRLK
PDNTDVEPYM VDEHIYLSEV SNGLKRHYYW APTYGETYMP SSSAGFALVP VGEHL
