LRC45_HUMAN
ID LRC45_HUMAN Reviewed; 670 AA.
AC Q96CN5;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Leucine-rich repeat-containing protein 45;
GN Name=LRRC45;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-661, AND INTERACTION WITH
RP CROCC AND CEP250.
RX PubMed=24035387; DOI=10.1016/j.celrep.2013.08.005;
RA He R., Huang N., Bao Y., Zhou H., Teng J., Chen J.;
RT "LRRC45 is a centrosome linker component required for centrosome
RT cohesion.";
RL Cell Rep. 4:1100-1107(2013).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=31974111; DOI=10.1242/jcs.239616;
RA Hossain D., Shih S.Y., Xiao X., White J., Tsang W.Y.;
RT "Cep44 functions in centrosome cohesion by stabilizing rootletin.";
RL J. Cell Sci. 133:0-0(2020).
CC -!- FUNCTION: Component of the proteinaceous fiber-like linker between two
CC centrioles, required for centrosome cohesion.
CC {ECO:0000269|PubMed:24035387}.
CC -!- SUBUNIT: Interacts with CROCC/rootletin and CEP250.
CC {ECO:0000269|PubMed:24035387}.
CC -!- INTERACTION:
CC Q96CN5; P13196: ALAS1; NbExp=3; IntAct=EBI-2805176, EBI-3905054;
CC Q96CN5; Q8N998: CCDC89; NbExp=3; IntAct=EBI-2805176, EBI-12814117;
CC Q96CN5; P42858: HTT; NbExp=3; IntAct=EBI-2805176, EBI-466029;
CC Q96CN5; Q9BQD3: KXD1; NbExp=4; IntAct=EBI-2805176, EBI-739657;
CC Q96CN5; P23508: MCC; NbExp=3; IntAct=EBI-2805176, EBI-307531;
CC Q96CN5; Q9UJV3-2: MID2; NbExp=3; IntAct=EBI-2805176, EBI-10172526;
CC Q96CN5; Q16385-2: SSX2B; NbExp=3; IntAct=EBI-2805176, EBI-17564583;
CC Q96CN5; Q7RTT5: SSX7; NbExp=3; IntAct=EBI-2805176, EBI-12879730;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:24035387,
CC ECO:0000269|PubMed:31974111}. Note=Localizes to the proteinaceous
CC linker between the proximal ends of the centrioles.
CC {ECO:0000269|PubMed:24035387}.
CC -!- PTM: Phosphorylated by NEK2 during misosis, phosphorylation reduces
CC centrosomal localization which subsequently leads to centrosome
CC separation. {ECO:0000269|PubMed:24035387}.
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DR EMBL; BC014109; AAH14109.1; -; mRNA.
DR CCDS; CCDS11797.1; -.
DR RefSeq; NP_659436.1; NM_144999.3.
DR AlphaFoldDB; Q96CN5; -.
DR SMR; Q96CN5; -.
DR BioGRID; 128377; 32.
DR DIP; DIP-29482N; -.
DR IntAct; Q96CN5; 19.
DR MINT; Q96CN5; -.
DR STRING; 9606.ENSP00000306760; -.
DR iPTMnet; Q96CN5; -.
DR PhosphoSitePlus; Q96CN5; -.
DR BioMuta; LRRC45; -.
DR DMDM; 74760765; -.
DR EPD; Q96CN5; -.
DR jPOST; Q96CN5; -.
DR MassIVE; Q96CN5; -.
DR MaxQB; Q96CN5; -.
DR PaxDb; Q96CN5; -.
DR PeptideAtlas; Q96CN5; -.
DR PRIDE; Q96CN5; -.
DR ProteomicsDB; 76198; -.
DR Antibodypedia; 19866; 116 antibodies from 21 providers.
DR DNASU; 201255; -.
DR Ensembl; ENST00000306688.8; ENSP00000306760.3; ENSG00000169683.8.
DR GeneID; 201255; -.
DR KEGG; hsa:201255; -.
DR MANE-Select; ENST00000306688.8; ENSP00000306760.3; NM_144999.4; NP_659436.1.
DR UCSC; uc002kde.4; human.
DR CTD; 201255; -.
DR GeneCards; LRRC45; -.
DR HGNC; HGNC:28302; LRRC45.
DR HPA; ENSG00000169683; Low tissue specificity.
DR neXtProt; NX_Q96CN5; -.
DR OpenTargets; ENSG00000169683; -.
DR PharmGKB; PA142671505; -.
DR VEuPathDB; HostDB:ENSG00000169683; -.
DR eggNOG; KOG4308; Eukaryota.
DR GeneTree; ENSGT00940000154003; -.
DR HOGENOM; CLU_015877_0_0_1; -.
DR InParanoid; Q96CN5; -.
DR OMA; EVDHMTR; -.
DR OrthoDB; 1456539at2759; -.
DR PhylomeDB; Q96CN5; -.
DR TreeFam; TF330983; -.
DR PathwayCommons; Q96CN5; -.
DR SignaLink; Q96CN5; -.
DR BioGRID-ORCS; 201255; 13 hits in 1075 CRISPR screens.
DR ChiTaRS; LRRC45; human.
DR GenomeRNAi; 201255; -.
DR Pharos; Q96CN5; Tdark.
DR PRO; PR:Q96CN5; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q96CN5; protein.
DR Bgee; ENSG00000169683; Expressed in right uterine tube and 97 other tissues.
DR ExpressionAtlas; Q96CN5; baseline and differential.
DR Genevisible; Q96CN5; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0030426; C:growth cone; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13516; LRR_6; 3.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Cytoskeleton; Leucine-rich repeat; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..670
FT /note="Leucine-rich repeat-containing protein 45"
FT /id="PRO_0000223923"
FT REPEAT 58..80
FT /note="LRR 1"
FT REPEAT 87..107
FT /note="LRR 2"
FT REPEAT 115..136
FT /note="LRR 3"
FT REPEAT 145..166
FT /note="LRR 4"
FT REPEAT 173..194
FT /note="LRR 5"
FT REPEAT 201..212
FT /note="LRR 6"
FT COILED 252..645
FT /evidence="ECO:0000255"
FT MOD_RES 661
FT /note="Phosphoserine; by NEK2"
FT /evidence="ECO:0000269|PubMed:24035387"
SQ SEQUENCE 670 AA; 75951 MW; 27F5D210721459FB CRC64;
MEEFRRSYSR LCRESGAEPQ EAVLQQLHQL PRGRLDLATQ SLTVETCRAL GKLLPRETLC
TELVLSDCML SEEGATLLLR GLCANTVLRF LDLKGNNLRA AGAEALGKLL QQNKSIQSLT
LEWNSLGTWD DAFATFCGGL AANGALQRLD LRNNQISHKG AEELALALKG NTTLQQLDLR
WNNVGLLGGR ALMNCLPSNR TLWRLDLAGN NIPGDVLRAV EQAMGHSQDR LTTFQENQAR
THVLSKEVQH LREEKSKQFL DLMETIDKQR EEMAKSSRAS AARVGQLQEA LNERHSIINA
LKAKLQMTEA ALALSEQKAQ DLGELLATAE QEQLSLSQRQ AKELKLEQQE AAERESKLLR
DLSAANEKNL LLQNQVDELE RKFRCQQEQL FQTRQEMTSM SAELKMRAIQ AEERLDMEKR
RCRQSLEDSE SLRIKEVEHM TRHLEESEKA MQERVQRLEA ARLSLEEELS RVKAAALSER
GQAEEELIKA KSQARLEEQQ RLAHLEDKLR LLAQARDEAQ GACLQQKQVV AEAQTRVSQL
GLQVEGLRRR LEELQQELSL KDQERVAEVS RVRVELQEQN GRLQAELAAQ EALREKAAAL
ERQLKVMASD HREALLDRES ENASLREKLR LREAEIARIR DEEAQRASFL QNAVLAYVQA
SPVRTLSPPK
