LRC45_MOUSE
ID LRC45_MOUSE Reviewed; 670 AA.
AC Q8CIM1; A2AC12; Q3U5Z2;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Leucine-rich repeat-containing protein 45;
GN Name=Lrrc45;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the proteinaceous fiber-like linker between two
CC centrioles, required for centrosome cohesion.
CC {ECO:0000250|UniProtKB:Q96CN5}.
CC -!- SUBUNIT: Interacts with CROCC/rootletin and CEP250.
CC {ECO:0000250|UniProtKB:Q96CN5}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250|UniProtKB:Q96CN5}. Note=Localizes to
CC the proteinaceous linker between the proximal ends of the centrioles.
CC {ECO:0000250|UniProtKB:Q96CN5}.
CC -!- PTM: Phosphorylated by NEK2 during misosis, phosphorylation reduces
CC centrosomal localization which subsequently leads to centrosome
CC separation. {ECO:0000250|UniProtKB:Q96CN5}.
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DR EMBL; AK153361; BAE31933.1; -; mRNA.
DR EMBL; AL663030; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466558; EDL34793.1; -; Genomic_DNA.
DR EMBL; BC023296; AAH23296.1; -; mRNA.
DR CCDS; CCDS25753.1; -.
DR RefSeq; NP_705773.2; NM_153545.2.
DR RefSeq; XP_006533198.1; XM_006533135.3.
DR AlphaFoldDB; Q8CIM1; -.
DR SMR; Q8CIM1; -.
DR BioGRID; 229902; 2.
DR STRING; 10090.ENSMUSP00000026139; -.
DR iPTMnet; Q8CIM1; -.
DR PhosphoSitePlus; Q8CIM1; -.
DR EPD; Q8CIM1; -.
DR MaxQB; Q8CIM1; -.
DR PaxDb; Q8CIM1; -.
DR PeptideAtlas; Q8CIM1; -.
DR PRIDE; Q8CIM1; -.
DR ProteomicsDB; 292026; -.
DR Antibodypedia; 19866; 116 antibodies from 21 providers.
DR Ensembl; ENSMUST00000026139; ENSMUSP00000026139; ENSMUSG00000025145.
DR GeneID; 217366; -.
DR KEGG; mmu:217366; -.
DR UCSC; uc007mue.2; mouse.
DR CTD; 201255; -.
DR MGI; MGI:2387183; Lrrc45.
DR VEuPathDB; HostDB:ENSMUSG00000025145; -.
DR eggNOG; KOG4308; Eukaryota.
DR GeneTree; ENSGT00940000154003; -.
DR HOGENOM; CLU_015877_0_0_1; -.
DR InParanoid; Q8CIM1; -.
DR OMA; EVDHMTR; -.
DR OrthoDB; 1456539at2759; -.
DR PhylomeDB; Q8CIM1; -.
DR TreeFam; TF330983; -.
DR BioGRID-ORCS; 217366; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Lrrc45; mouse.
DR PRO; PR:Q8CIM1; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8CIM1; protein.
DR Bgee; ENSMUSG00000025145; Expressed in bone fossa and 225 other tissues.
DR ExpressionAtlas; Q8CIM1; baseline and differential.
DR Genevisible; Q8CIM1; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0030426; C:growth cone; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13516; LRR_6; 3.
PE 2: Evidence at transcript level;
KW Coiled coil; Cytoplasm; Cytoskeleton; Leucine-rich repeat; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..670
FT /note="Leucine-rich repeat-containing protein 45"
FT /id="PRO_0000223924"
FT REPEAT 59..79
FT /note="LRR 1"
FT REPEAT 87..108
FT /note="LRR 2"
FT REPEAT 115..136
FT /note="LRR 3"
FT REPEAT 145..166
FT /note="LRR 4"
FT REPEAT 173..194
FT /note="LRR 5"
FT REPEAT 201..223
FT /note="LRR 6"
FT REGION 332..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 235..645
FT /evidence="ECO:0000255"
FT COMPBIAS 334..352
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 661
FT /note="Phosphoserine; by NEK2"
FT /evidence="ECO:0000250|UniProtKB:Q96CN5"
FT CONFLICT 366
FT /note="S -> N (in Ref. 2; AAH23296)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 670 AA; 76384 MW; EDC0D0321D6D6485 CRC64;
MEEFRRSYNR LCEESGAEPQ EAVLQQLHQL PKGGLDLTTQ SLTVETCRAL GKLLHKETLL
KELVLSDCML SEEGSTLLFQ GLCANTSVQH LDLKGNNLRA TGAEALGKLL RQNKSIQSLT
LEWNNLGTWE DAFATFCGGL AANSALRQLD LRNNQISHKG AEELALALKG NTTLQQLDLR
WNNIGLLGGR ALVNCLPSNR TLWKLDLAGN NIPGDILRAV EQAMDHNQDR LTAFRENQAR
TKILSKEVQH LQEEKSKQFL DLMETIDKQR EEMARDSRAS AVRVGQLQEA LNERQSIINA
LKAKLQMTEA ALALSEQKVR DLGELLVAGD QERQSLSQRH EKERKLERQE AADRESKLLR
DLSAASEKNL LLRSQVDELE RKARSQQEQL FLTKQELTNT SAELKIRAIQ AEERLDVEKR
RAKQNMEDLE KLHSKEVDHM TRHLEESERA MQERVQRLEA LRLSLEEELS RMKAAVLSER
GQAEEELIKA RNQARLEEQH RLAHLEEKIR LLAQARDEAQ GTCVQQKQMV AESQARVSQL
NLQMEGQQRR LEELQQELIN KDQEKVAEVA RVRVELQEQM GRMQADLVAQ EALREKVAAL
ERQMKVIGSE HREALLDRES ENASLREKLR LKEAEISRIR DEEAQRASFL QNAVLAYVQG
SPLRALSPPK
