LRC49_MOUSE
ID LRC49_MOUSE Reviewed; 686 AA.
AC Q91YK0; Q8C3S4; Q8C7T7;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Leucine-rich repeat-containing protein 49;
DE AltName: Full=Tubulin polyglutamylase complex subunit 4;
DE Short=PGs4;
DE AltName: Full=p79;
GN Name=Lrrc49;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 340-347, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY AS PART OF THE TUBULIN POLYGLUTAMYLASE
RP COMPLEX.
RX PubMed=15890843; DOI=10.1126/science.1113010;
RA Janke C., Rogowski K., Wloga D., Regnard C., Kajava A.V., Strub J.-M.,
RA Temurak N., van Dijk J., Boucher D., van Dorsselaer A., Suryavanshi S.,
RA Gaertig J., Edde B.;
RT "Tubulin polyglutamylase enzymes are members of the TTL domain protein
RT family.";
RL Science 308:1758-1762(2005).
CC -!- FUNCTION: Subunit of the tubulin polyglutamylase complex (TPGC). The
CC complex mediates cilia and flagella polyglutamylation which is
CC essential for their biogenesis and motility.
CC {ECO:0000250|UniProtKB:Q8IUZ0}.
CC -!- SUBUNIT: Part of the neuronal tubulin polyglutamylase complex which
CC contains TPGS1, TPGS2, TTLL1, LRRC49 and NICN1 (PubMed:15890843).
CC Interacts with PCM1; TTLL1, TPGS1, TPGS2 and LRRC49 (By similarity).
CC {ECO:0000250, ECO:0000250|UniProtKB:Q8IUZ0,
CC ECO:0000269|PubMed:15890843}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q8IUZ0}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome, centriolar satellite
CC {ECO:0000250|UniProtKB:Q8IUZ0}. Note=Associated with microtubules.
CC {ECO:0000250|UniProtKB:Q8IUZ0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=Named isoforms=2.;
CC Name=1;
CC IsoId=Q91YK0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q91YK0-2; Sequence=VSP_018023;
CC Name=3;
CC IsoId=Q91YK0-3; Sequence=VSP_020408, VSP_020409;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC39342.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK049268; BAC33646.1; -; mRNA.
DR EMBL; AK085022; BAC39342.1; ALT_FRAME; mRNA.
DR EMBL; BC016574; AAH16574.1; -; mRNA.
DR CCDS; CCDS23257.1; -. [Q91YK0-2]
DR CCDS; CCDS52822.1; -. [Q91YK0-1]
DR RefSeq; NP_001139518.1; NM_001146046.1.
DR RefSeq; NP_001139519.1; NM_001146047.1.
DR RefSeq; NP_001298024.1; NM_001311095.1.
DR RefSeq; NP_663591.3; NM_145616.4.
DR RefSeq; XP_006510816.1; XM_006510753.2.
DR RefSeq; XP_017168581.1; XM_017313092.1.
DR AlphaFoldDB; Q91YK0; -.
DR SMR; Q91YK0; -.
DR CORUM; Q91YK0; -.
DR STRING; 10090.ENSMUSP00000070606; -.
DR iPTMnet; Q91YK0; -.
DR PhosphoSitePlus; Q91YK0; -.
DR MaxQB; Q91YK0; -.
DR PRIDE; Q91YK0; -.
DR ProteomicsDB; 292027; -. [Q91YK0-1]
DR ProteomicsDB; 292028; -. [Q91YK0-2]
DR ProteomicsDB; 292029; -. [Q91YK0-3]
DR DNASU; 102747; -.
DR GeneID; 102747; -.
DR KEGG; mmu:102747; -.
DR CTD; 54839; -.
DR MGI; MGI:2442689; Lrrc49.
DR InParanoid; Q91YK0; -.
DR OrthoDB; 955025at2759; -.
DR PhylomeDB; Q91YK0; -.
DR BioGRID-ORCS; 102747; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Lrrc49; mouse.
DR PRO; PR:Q91YK0; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q91YK0; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR GO; GO:0036158; P:outer dynein arm assembly; IBA:GO_Central.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR SMART; SM00369; LRR_TYP; 5.
DR PROSITE; PS51450; LRR; 7.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton;
KW Direct protein sequencing; Leucine-rich repeat; Microtubule;
KW Reference proteome; Repeat.
FT CHAIN 1..686
FT /note="Leucine-rich repeat-containing protein 49"
FT /id="PRO_0000233035"
FT REPEAT 113..134
FT /note="LRR 1"
FT REPEAT 135..156
FT /note="LRR 2"
FT REPEAT 157..178
FT /note="LRR 3"
FT REPEAT 179..200
FT /note="LRR 4"
FT REPEAT 201..222
FT /note="LRR 5"
FT REPEAT 223..244
FT /note="LRR 6"
FT REPEAT 245..266
FT /note="LRR 7"
FT DOMAIN 279..317
FT /note="LRRCT"
FT REGION 311..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 359..381
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 319..341
FT /evidence="ECO:0000255"
FT VAR_SEQ 66
FT /note="D -> DGRVISPSPRNKSSCISSTTNVTDLCGLDMRAATTSLSAFGDSSIQT
FT PSKPRIRQGCHSAGPNVSGD (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_018023"
FT VAR_SEQ 336..362
FT /note="EKKRLTINNVARKWDLQQRVANIASTQ -> VHPSVRPPLAQEDTSTLASTQ
FT EHGFQN (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_020408"
FT VAR_SEQ 363..686
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_020409"
FT CONFLICT 318
FT /note="P -> A (in Ref. 1; BAC33646)"
FT /evidence="ECO:0000305"
FT CONFLICT 452
FT /note="D -> G (in Ref. 1; BAC33646)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 686 AA; 78876 MW; D4B6B50B065B6C6D CRC64;
MIPGKYRSVS GRAANNVNCG LHLVIQTSSL SDKNKVEFRL NRETPPFPGR LLQHDLERNY
SSRQGDHISL VSSSMPAFPI LQRSSEEKTL YSDRLTLERQ KLTVCPIIDG EEHLRLLNFQ
HNFITRIQNI SNLQRLIFLD LYDNQIEEIS GLSTLKSLRV LLLGKNRIKK ISNLENLKNL
DVLDLHGNQI TKIENVNHLC DLRVLNLARN LLSHVDNLNG LDSLTELNLR HNQITFVRDV
DNLPCLQRLF LSFNNITSFE SVSCLAESTS LSDITFDGNP IAQESWYKHT VLQNMMQLRQ
LDMKRITEEE RRVASVVPKK EEEKKRESHK QSLLKEKKRL TINNVARKWD LQQRVANIAS
TQDRKDSESP PQESCQLDGG NISAFPEEAG SLDAGLSSAL QGLSVTETHL VEIDGETLSL
YGSGALECLD RNWSVQTAGM VTTVSFTFIE FDEIVQVLPK LKMKFPNSLH LKFRETNLVM
LQQFNALAQL RRVDQLTIDP QGNPVVNFTL WKYYVLFRLS HFSMQKINGT EVTQNDMIMA
ERLFGILAHV ASSELPQYRM ISILGDARKK QFRYLLESKG KKPGIICEET SDSKRFLGEN
TNRATLNYTT REFYHEKLEE IKDKKKFCRL YVEDLVKEAT AINMKNEALQ KLWPQMFIEL
VRDAVIEIRS KDSYMKLCLQ QITDQK
