LRCH2_MOUSE
ID LRCH2_MOUSE Reviewed; 773 AA.
AC Q3UMG5; A2AMN8; Q7TNP9; Q80TC9;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Leucine-rich repeat and calponin homology domain-containing protein 2;
GN Name=Lrch2; Synonyms=Kiaa1495;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 48-456 (ISOFORM 1).
RC TISSUE=Mammary gland;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 318-773 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 496-773 (ISOFORM 3).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
CC -!- FUNCTION: May play a role in the organization of the cytoskeleton.
CC {ECO:0000250|UniProtKB:Q960C5, ECO:0000250|UniProtKB:Q96II8}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q3UMG5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UMG5-2; Sequence=VSP_021041;
CC Name=3;
CC IsoId=Q3UMG5-3; Sequence=VSP_021042;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65798.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=CAM22136.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAM24878.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL671981; CAM22136.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL807780; CAM22136.1; JOINED; Genomic_DNA.
DR EMBL; AL807780; CAM24878.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL671981; CAM24878.1; JOINED; Genomic_DNA.
DR EMBL; AK144916; BAE26133.1; -; mRNA.
DR EMBL; BC056180; AAH56180.2; -; mRNA.
DR EMBL; AK122516; BAC65798.1; ALT_SEQ; mRNA.
DR CCDS; CCDS41160.1; -. [Q3UMG5-1]
DR RefSeq; NP_001074642.1; NM_001081173.1. [Q3UMG5-1]
DR RefSeq; XP_006528853.2; XM_006528790.3. [Q3UMG5-3]
DR RefSeq; XP_006528854.2; XM_006528791.3. [Q3UMG5-2]
DR AlphaFoldDB; Q3UMG5; -.
DR SMR; Q3UMG5; -.
DR BioGRID; 229146; 3.
DR IntAct; Q3UMG5; 2.
DR STRING; 10090.ENSMUSP00000123633; -.
DR iPTMnet; Q3UMG5; -.
DR PhosphoSitePlus; Q3UMG5; -.
DR EPD; Q3UMG5; -.
DR MaxQB; Q3UMG5; -.
DR PaxDb; Q3UMG5; -.
DR PRIDE; Q3UMG5; -.
DR ProteomicsDB; 292359; -. [Q3UMG5-1]
DR ProteomicsDB; 292360; -. [Q3UMG5-2]
DR ProteomicsDB; 292361; -. [Q3UMG5-3]
DR Antibodypedia; 517; 50 antibodies from 14 providers.
DR Ensembl; ENSMUST00000112819; ENSMUSP00000108438; ENSMUSG00000031290. [Q3UMG5-1]
DR GeneID; 210297; -.
DR KEGG; mmu:210297; -.
DR UCSC; uc009unk.1; mouse. [Q3UMG5-1]
DR CTD; 57631; -.
DR MGI; MGI:2147870; Lrch2.
DR VEuPathDB; HostDB:ENSMUSG00000031290; -.
DR eggNOG; KOG0532; Eukaryota.
DR GeneTree; ENSGT00940000160039; -.
DR HOGENOM; CLU_008231_1_0_1; -.
DR InParanoid; Q3UMG5; -.
DR OMA; DGQQSSH; -.
DR OrthoDB; 378148at2759; -.
DR PhylomeDB; Q3UMG5; -.
DR BioGRID-ORCS; 210297; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Lrch2; mouse.
DR PRO; PR:Q3UMG5; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q3UMG5; protein.
DR Bgee; ENSMUSG00000031290; Expressed in cortical plate and 173 other tissues.
DR ExpressionAtlas; Q3UMG5; baseline and differential.
DR Genevisible; Q3UMG5; MM.
DR Gene3D; 1.10.418.10; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF13855; LRR_8; 2.
DR SMART; SM00033; CH; 1.
DR SMART; SM00369; LRR_TYP; 5.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS51450; LRR; 8.
PE 2: Evidence at transcript level;
KW Alternative splicing; Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..773
FT /note="Leucine-rich repeat and calponin homology domain-
FT containing protein 2"
FT /id="PRO_0000253483"
FT REPEAT 97..118
FT /note="LRR 1"
FT REPEAT 120..141
FT /note="LRR 2"
FT REPEAT 143..164
FT /note="LRR 3"
FT REPEAT 166..187
FT /note="LRR 4"
FT REPEAT 188..209
FT /note="LRR 5"
FT REPEAT 211..232
FT /note="LRR 6"
FT REPEAT 234..256
FT /note="LRR 7"
FT REPEAT 257..277
FT /note="LRR 8"
FT REPEAT 279..300
FT /note="LRR 9"
FT DOMAIN 650..763
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 324..409
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 438..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 573..633
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..343
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 372..399
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 441..466
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 573..592
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 593..607
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 496..517
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_021041"
FT VAR_SEQ 589..605
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12693553"
FT /id="VSP_021042"
FT CONFLICT 455
FT /note="P -> L (in Ref. 2; BAE26133)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 773 AA; 84989 MW; 7C8A85C41AF749FF CRC64;
MAASQGGGGN SGGGGCSGGG SGGGGGAAGG GGGGGGGGGG GAGAGGGGGC GGTVAVPIPV
PTLFGQPFPN GPQWHPGSLQ PQHTVRSLDR ALEEAGNSGI LSLSGRKLRE FPGSGYDLTD
TTQADLSRNR FTEIPSDVWL FAPLETLNLY HNCIKTIPEA IKNLQMLTYL NISRNLLSTL
PKYLFDLPLK VLVVSNNKLV SIPEEIGKLK DLMELDVSCN EIQVLPQQMG KLHSLKELNI
RRNNLHVLPD ELGDLPLVKL DFSCNKVTEI PVCYRKLHHL QVIILDNNPL QVPPAQICLK
GKVHIFKYLN IQACCRMDKK PDSLDLPSLN KRMPSQPLTD SMEDFYPNKN HGPDSGIGSD
NGEKRLSTTE PSDDDTISLH SQVSESNREQ TSRNDSHITG SKPDSQKDQE VYDFIDPNTE
DVAVPEEGDT HIGSFVSFLK GKEKSSEKSQ KNEEPPNEKK VDKEQLLPEE EDDDLKEVTD
LRKIAAQLLK QEQKNRILNH STSVMRNKLK QTVECEKSVP ADEGNSPLSP LAWQPLENQK
DQIVDQQWPE SQPIIWQNEE RRRSKQIRKE YFKYKSTRKN SSGNENEEQE SDNAHMSAQS
PVSSEEYDRS DGFSHGPFGL KPRSAFSRAS RQEYGAADPG FTMRRKMEHL REEREQIRQL
RNNLESRLKV ILPDDIGAAL MDGVVLCHLA NHIRPRSVAS IHVPSPAVPK LSMAKCRRNV
ENFLDACKKL GVSQERLCLP HHILEERGLV KVGVTVQALL ELPTTKASQL SMA
