LRCH4_HUMAN
ID LRCH4_HUMAN Reviewed; 683 AA.
AC O75427; A4D2D5; Q8WV85; Q96ID0;
DT 21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2004, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Leucine-rich repeat and calponin homology domain-containing protein 4;
DE AltName: Full=Leucine-rich repeat neuronal protein 4;
DE Short=Leucine-rich neuronal protein;
GN Name=LRCH4; Synonyms=LRN, LRRN1, LRRN4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9799793; DOI=10.1101/gr.8.10.1060;
RA Gloeckner G., Scherer S., Schattevoy R., Boright A.P., Weber J.,
RA Tsui L.-C., Rosenthal A.;
RT "Large-scale sequencing of two regions in human chromosome 7q22: analysis
RT of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17
RT genes.";
RL Genome Res. 8:1060-1073(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT MET-642.
RC TISSUE=Muscle, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-457; SER-511; SER-517 AND
RP SER-521, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304; SER-307; SER-309;
RP SER-432; SER-457; SER-513; SER-521 AND SER-589, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- INTERACTION:
CC O75427; P00387: CYB5R3; NbExp=3; IntAct=EBI-718707, EBI-1046040;
CC O75427; Q96HP0: DOCK6; NbExp=2; IntAct=EBI-718707, EBI-4401295;
CC O75427; Q96N67: DOCK7; NbExp=2; IntAct=EBI-718707, EBI-2433703;
CC O75427; Q8NF50-3: DOCK8; NbExp=2; IntAct=EBI-718707, EBI-25409271;
CC O75427; A1L3X0: ELOVL7; NbExp=3; IntAct=EBI-718707, EBI-10285373;
CC O75427; Q8NBQ5: HSD17B11; NbExp=3; IntAct=EBI-718707, EBI-1052304;
CC O75427; Q8N6L0: KASH5; NbExp=3; IntAct=EBI-718707, EBI-749265;
CC O75427; Q6A162: KRT40; NbExp=3; IntAct=EBI-718707, EBI-10171697;
CC O75427; P60409: KRTAP10-7; NbExp=3; IntAct=EBI-718707, EBI-10172290;
CC O75427; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-718707, EBI-10171774;
CC O75427; Q99750: MDFI; NbExp=3; IntAct=EBI-718707, EBI-724076;
CC O75427; Q7Z3S9: NOTCH2NLA; NbExp=3; IntAct=EBI-718707, EBI-945833;
CC O75427; Q86VR2: RETREG3; NbExp=3; IntAct=EBI-718707, EBI-10192441;
CC O75427; Q6P5S7: RNASEK; NbExp=3; IntAct=EBI-718707, EBI-18397230;
CC O75427; Q9Y2Z0: SUGT1; NbExp=2; IntAct=EBI-718707, EBI-307008;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC78793.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF053356; AAC78793.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CH236956; EAL23828.1; -; Genomic_DNA.
DR EMBL; BC007634; AAH07634.1; -; mRNA.
DR EMBL; BC018529; AAH18529.3; -; mRNA.
DR CCDS; CCDS34706.1; -.
DR RefSeq; NP_001276863.1; NM_001289934.1.
DR RefSeq; NP_002310.2; NM_002319.4.
DR AlphaFoldDB; O75427; -.
DR SMR; O75427; -.
DR BioGRID; 110214; 31.
DR IntAct; O75427; 24.
DR STRING; 9606.ENSP00000309689; -.
DR GlyGen; O75427; 2 sites, 1 O-linked glycan (2 sites).
DR iPTMnet; O75427; -.
DR PhosphoSitePlus; O75427; -.
DR BioMuta; LRCH4; -.
DR EPD; O75427; -.
DR jPOST; O75427; -.
DR MassIVE; O75427; -.
DR MaxQB; O75427; -.
DR PaxDb; O75427; -.
DR PeptideAtlas; O75427; -.
DR PRIDE; O75427; -.
DR ProteomicsDB; 50000; -.
DR Antibodypedia; 30732; 161 antibodies from 21 providers.
DR DNASU; 4034; -.
DR Ensembl; ENST00000310300.11; ENSP00000309689.6; ENSG00000077454.17.
DR GeneID; 4034; -.
DR KEGG; hsa:4034; -.
DR MANE-Select; ENST00000310300.11; ENSP00000309689.6; NM_002319.5; NP_002310.2.
DR UCSC; uc003uvj.4; human.
DR CTD; 4034; -.
DR DisGeNET; 4034; -.
DR GeneCards; LRCH4; -.
DR HGNC; HGNC:6691; LRCH4.
DR HPA; ENSG00000077454; Low tissue specificity.
DR neXtProt; NX_O75427; -.
DR OpenTargets; ENSG00000077454; -.
DR PharmGKB; PA30467; -.
DR VEuPathDB; HostDB:ENSG00000077454; -.
DR eggNOG; KOG0532; Eukaryota.
DR GeneTree; ENSGT00940000161668; -.
DR HOGENOM; CLU_008231_2_0_1; -.
DR InParanoid; O75427; -.
DR OMA; LSTQATY; -.
DR OrthoDB; 378148at2759; -.
DR PhylomeDB; O75427; -.
DR TreeFam; TF318428; -.
DR PathwayCommons; O75427; -.
DR SignaLink; O75427; -.
DR BioGRID-ORCS; 4034; 14 hits in 1078 CRISPR screens.
DR ChiTaRS; LRCH4; human.
DR GeneWiki; LRCH4; -.
DR GenomeRNAi; 4034; -.
DR Pharos; O75427; Tbio.
DR PRO; PR:O75427; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; O75427; protein.
DR Bgee; ENSG00000077454; Expressed in granulocyte and 95 other tissues.
DR ExpressionAtlas; O75427; baseline and differential.
DR Genevisible; O75427; HS.
DR GO; GO:0005737; C:cytoplasm; IEA:Ensembl.
DR GO; GO:0016605; C:PML body; IDA:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:Ensembl.
DR GO; GO:0007399; P:nervous system development; TAS:ProtInc.
DR CDD; cd00014; CH; 1.
DR Gene3D; 1.10.418.10; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF13855; LRR_8; 2.
DR SMART; SM00033; CH; 1.
DR SMART; SM00369; LRR_TYP; 5.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS51450; LRR; 7.
PE 1: Evidence at protein level;
KW Leucine-rich repeat; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..683
FT /note="Leucine-rich repeat and calponin homology domain-
FT containing protein 4"
FT /id="PRO_0000084480"
FT REPEAT 43..64
FT /note="LRR 1"
FT REPEAT 69..90
FT /note="LRR 2"
FT REPEAT 92..114
FT /note="LRR 3"
FT REPEAT 115..136
FT /note="LRR 4"
FT REPEAT 137..158
FT /note="LRR 5"
FT REPEAT 160..182
FT /note="LRR 6"
FT REPEAT 183..205
FT /note="LRR 7"
FT REPEAT 206..226
FT /note="LRR 8"
FT REPEAT 228..249
FT /note="LRR 9"
FT DOMAIN 534..647
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 449..539
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 326..349
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..413
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 450..467
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 478..492
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 504..522
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 279
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921G6"
FT MOD_RES 281
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921G6"
FT MOD_RES 304
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 307
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 309
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 313
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921G6"
FT MOD_RES 432
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 457
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 511
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 513
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 517
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 521
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 589
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VARIANT 642
FT /note="V -> M (in dbSNP:rs3197597)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_051135"
FT CONFLICT 40
FT /note="E -> D (in Ref. 1; AAC78793)"
FT /evidence="ECO:0000305"
FT CONFLICT 391
FT /note="S -> N (in Ref. 1; AAC78793)"
FT /evidence="ECO:0000305"
FT CONFLICT 467..473
FT /note="AAAGQGA -> GCSGAGS (in Ref. 1; AAC78793)"
FT /evidence="ECO:0000305"
FT CONFLICT 682..683
FT /note="GS -> DP (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 683 AA; 73450 MW; AF297AA30E4DA353 CRC64;
MAAAVAAPLA AGGEEAAATT SVPGSPGLPG RRSAERALEE AVATGTLNLS NRRLKHFPRG
AARSYDLSDI TQADLSRNRF PEVPEAACQL VSLEGLSLYH NCLRCLNPAL GNLTALTYLN
LSRNQLSLLP PYICQLPLRV LIVSNNKLGA LPPDIGTLGS LRQLDVSSNE LQSLPSELCG
LSSLRDLNVR RNQLSTLPEE LGDLPLVRLD FSCNRVSRIP VSFCRLRHLQ VILLDSNPLQ
SPPAQVCLKG KLHIFKYLST EAGQRGSALG DLAPSRPPSF SPCPAEDLFP GHRYDGGLDS
GFHSVDSGSK RWSGNESTDE FSELSFRISE LAREPRGPRE RKEDGSADGD PVQIDFIDSH
VPGEDEERGT VEEQRPPELS PGAGDRERAP SSRREEPAGE ERRRPDTLQL WQERERRQQQ
QSGAWGAPRK DSLLKPGLRA VVGGAAAVST QAMHNGSPKS SASQAGAAAG QGAPAPAPAS
QEPLPIAGPA TAPAPRPLGS IQRPNSFLFR SSSQSGSGPS SPDSVLRPRR YPQVPDEKDL
MTQLRQVLES RLQRPLPEDL AEALASGVIL CQLANQLRPR SVPFIHVPSP AVPKLSALKA
RKNVESFLEA CRKMGVPEAD LCSPSDLLQG TARGLRTALE AVKRVGGKAL PPLWPPSGLG
GFVVFYVVLM LLLYVTYTRL LGS
