LRCH_DROME
ID LRCH_DROME Reviewed; 809 AA.
AC Q960C5;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Leucine-rich repeat and calponin homology domain-containing protein {ECO:0000305};
DE Short=dLRCH {ECO:0000303|PubMed:20805893};
GN Name=Lrch {ECO:0000312|FlyBase:FBgn0032633};
GN ORFNames=CG6860 {ECO:0000312|EMBL:AAK93550.1};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAK93550.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=20805893; DOI=10.1371/journal.pone.0012257;
RA Foussard H., Ferrer P., Valenti P., Polesello C., Carreno S., Payre F.;
RT "LRCH proteins: a novel family of cytoskeletal regulators.";
RL PLoS ONE 5:E12257-E12257(2010).
CC -!- FUNCTION: May play a role in the stabilization of the actin-rich cell
CC cortex during cell division. {ECO:0000269|PubMed:20805893}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:20805893}. Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:20805893}. Cleavage furrow
CC {ECO:0000269|PubMed:20805893}. Note=Associated with the actin network
CC at the onset of mitosis (PubMed:20805893). Associated with spindle
CC microtubules in metaphase (PubMed:20805893).
CC {ECO:0000269|PubMed:20805893}.
CC -!- DEVELOPMENTAL STAGE: There is a maternal contribution to the expression
CC during the early stages of embryogenesis (PubMed:20805893). Zygotic
CC expression is ubiquitous but reinforced in several embryonic tissues
CC (PubMed:20805893). From stage 11 to 12, accumulates in the developing
CC tracheal system, then in subsets of cells composing the central nervous
CC system (PubMed:20805893). Finally, from stage-15, it is also strongly
CC expressed in the gonads (PubMed:20805893).
CC {ECO:0000269|PubMed:20805893}.
CC -!- DISRUPTION PHENOTYPE: Embryos homozygous for a null allele of the gene
CC are viable and do not display gross developmental defects, developing
CC to adult stage, albeit with a slightly decreased viability
CC (PubMed:20805893). Homozygous females are sterile, laying rare embryos
CC that display no sign of development (PubMed:20805893). Mutant flies
CC display increased lethality at extreme temperatures (PubMed:20805893).
CC {ECO:0000269|PubMed:20805893}.
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DR EMBL; AY052126; AAK93550.1; -; mRNA.
DR EMBL; AE014134; AAN10986.1; -; Genomic_DNA.
DR RefSeq; NP_724046.1; NM_165212.2.
DR AlphaFoldDB; Q960C5; -.
DR SMR; Q960C5; -.
DR IntAct; Q960C5; 1.
DR STRING; 7227.FBpp0080522; -.
DR DNASU; 35041; -.
DR EnsemblMetazoa; FBtr0080968; FBpp0080521; FBgn0032633.
DR GeneID; 35041; -.
DR KEGG; dme:Dmel_CG6860; -.
DR UCSC; CG6860-RA; d. melanogaster.
DR CTD; 35041; -.
DR FlyBase; FBgn0032633; Lrch.
DR VEuPathDB; VectorBase:FBgn0032633; -.
DR GeneTree; ENSGT00940000158330; -.
DR OMA; LYSWIFP; -.
DR SignaLink; Q960C5; -.
DR BioGRID-ORCS; 35041; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35041; -.
DR PRO; PR:Q960C5; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032633; Expressed in cleaving embryo and 67 other tissues.
DR ExpressionAtlas; Q960C5; baseline and differential.
DR GO; GO:0005938; C:cell cortex; IDA:FlyBase.
DR GO; GO:0032154; C:cleavage furrow; IDA:FlyBase.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0007010; P:cytoskeleton organization; ISS:FlyBase.
DR Gene3D; 1.10.418.10; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF13855; LRR_8; 1.
DR SMART; SM00033; CH; 1.
DR SMART; SM00369; LRR_TYP; 6.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS51450; LRR; 7.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoskeleton; Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..809
FT /note="Leucine-rich repeat and calponin homology domain-
FT containing protein"
FT /id="PRO_0000447859"
FT REPEAT 72..96
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 98..121
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 122..144
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 145..168
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 170..189
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 191..213
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 214..236
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 238..258
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 260..282
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT DOMAIN 662..776
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT REGION 27..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 295..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 423..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 490..550
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 783..809
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 315..351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 423..439
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 490..544
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 809 AA; 87106 MW; 663DEDF3C70661E9 CRC64;
MAATVYQRLH SVAVGVGSAA GSAIGSGSAS LPGSGTGSGS GIGHAGNTSS STSSALLSHS
QLTRSLERIL EEAHFSGELI LTNRKLKDFP RTGTKYSLTD TVIADLSRNR FAELPEEVTT
FAFLETLLLY HNTIRSIPES VKQLSSLTYL DLRSNQLSSL PREICFLPLQ VLLVSNNRLT
SLPDELGRLD QTLTDLDASY NQLANLPARL GELRSLRSLS LRSNHLLYLP RELTCLSLIS
LDVSNNKIAS LPLEIRHMST LVELQLENNP LTSPPASLCM RGLVHVFKFL ETQATKDEKG
SRSGGNYDGY TTLRRAPRHN SSGNVLEAST TGSTNNQRRH QVDSGYNTSD GLDKRWSHDA
PTKSKTDSPL HCVSNSTAAT LPRTLPSAVH SQADLMDASL TSSTSTIVDE SLTLSPTASP
CKLSYAHSNS SSNGSSPDQD DDIMLDHQER TVHHANGRSG KGLGNIQTYK EYKEALKQQR
NQEISVYKQK HPNANHSPND DEDLSPSPPS ISNGSSSNTL PKSIMKQNSN QIGHNGNGNG
SANGNGVDDV VIPKKPIQKV IPSRITEIKP ASTSDIRSAN SSDCLGYVKP QSPMKNGTSK
FNTSNGGGVQ TTVGIVSSTT IKSPVSSTTK LGTVKTHRSV TWNHDIPAEK LSFTMRREFD
RQREETELMS QLRTIIETRL KMTLPVDIAS ALTDGVILCH LANYVRPRSV ASIHVPSPGV
NKLTMARCRR NVDNFLEACR RIGVDENLIC CAADVLEGKG AVQVAITVGE LFRLHGNGGC
GSGNSSGAAT PTKSPTRTTR ATMSPTPLA
