LRE_ARATH
ID LRE_ARATH Reviewed; 165 AA.
AC B3GS44; A0A0G7ZNQ5;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=GPI-anchored protein LORELEI;
DE Flags: Precursor;
GN Name=LRE; OrderedLocusNames=At4g26466; ORFNames=M3E9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=19028964; DOI=10.1105/tpc.108.061713;
RA Capron A., Gourgues M., Neiva L.S., Faure J.-E., Berger F., Pagnussat G.,
RA Krishnan A., Alvarez-Mejia C., Vielle-Calzada J.-P., Lee Y.-R., Liu B.,
RA Sundaresan V.;
RT "Maternal control of male-gamete delivery in Arabidopsis involves a
RT putative GPI-anchored protein encoded by the LORELEI gene.";
RL Plant Cell 20:3038-3049(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=20163554; DOI=10.1111/j.1365-313x.2010.04177.x;
RA Tsukamoto T., Qin Y., Huang Y., Dunatunga D., Palanivelu R.;
RT "A role for LORELEI, a putative glycosylphosphatidylinositol-anchored
RT protein, in Arabidopsis thaliana double fertilization and early seed
RT development.";
RL Plant J. 62:571-588(2010).
RN [5]
RP FUNCTION, INTERACTION WITH FER, AND TISSUE SPECIFICITY.
RX PubMed=26052747; DOI=10.7554/elife.06587;
RA Li C., Yeh F.L., Cheung A.Y., Duan Q., Kita D., Liu M.C., Maman J.,
RA Luu E.J., Wu B.W., Gates L., Jalal M., Kwong A., Carpenter H., Wu H.M.;
RT "Glycosylphosphatidylinositol-anchored proteins as chaperones and co-
RT receptors for FERONIA receptor kinase signaling in Arabidopsis.";
RL Elife 4:0-0(2015).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF 82-PRO--ASP-93 AND
RP 139-SER--ALA-141.
RX PubMed=27081182; DOI=10.1105/tpc.15.00703;
RA Liu X., Castro C., Wang Y., Noble J., Ponvert N., Bundy M., Hoel C.,
RA Shpak E., Palanivelu R.;
RT "The role of LORELEI in pollen tube reception at the interface of the
RT synergid cell and pollen tube requires the modified eight-cysteine motif
RT and the receptor-like kinase FERONIA.";
RL Plant Cell 28:1035-1052(2016).
CC -!- FUNCTION: Female gametophyte-specific component of the signaling
CC pathway required for fertilization. Required for reception of the
CC pollen tube by the female gametophyte (PubMed:19028964,
CC PubMed:26052747, PubMed:27081182). Acts specifically at the synergid
CC cell surface for pollen tube reception (PubMed:26052747,
CC PubMed:27081182). Plays a role in double fertilization and early seed
CC development (PubMed:20163554). Component of the FER-regulated Rho
CC GTPase signaling complex. Acts as a chaperone and coreceptor for FER.
CC Required for localization of FER to the plasma membrane
CC (PubMed:26052747). {ECO:0000269|PubMed:19028964,
CC ECO:0000269|PubMed:20163554, ECO:0000269|PubMed:26052747,
CC ECO:0000269|PubMed:27081182}.
CC -!- SUBUNIT: Interacts with FER. {ECO:0000269|PubMed:26052747}.
CC -!- INTERACTION:
CC B3GS44; Q9FN92: At5g59700; NbExp=2; IntAct=EBI-21914693, EBI-17071988;
CC B3GS44; Q9LX66: HERK1; NbExp=2; IntAct=EBI-21914693, EBI-22028097;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:27081182};
CC Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:27081182}. Note=Localized
CC to the filiform apparatus of the synergid cell.
CC {ECO:0000269|PubMed:27081182}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, buds, flowers and stems
CC (PubMed:19028964). Highest expression in the synergid cells of the
CC female gametophyte (PubMed:19028964, PubMed:26052747).
CC {ECO:0000269|PubMed:19028964, ECO:0000269|PubMed:26052747}.
CC -!- DEVELOPMENTAL STAGE: Expressed only in fully differentiated cells of
CC the egg apparatus. Not detected in developing ovules undergoing
CC megasporogenesis or megagametogenesis (PubMed:19028964). Expressed in
CC unfertilized, mature ovules (PubMed:20163554).
CC {ECO:0000269|PubMed:19028964, ECO:0000269|PubMed:20163554}.
CC -!- DISRUPTION PHENOTYPE: Failure of pollen tube arrest and fertilization.
CC Deficient in pollen tube reception by the female gametophyte.
CC {ECO:0000269|PubMed:19028964}.
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DR EMBL; EU727071; ACD75469.1; -; mRNA.
DR EMBL; BK006574; DAA64759.1; -; Genomic_DNA.
DR EMBL; AL022223; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL161565; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002687; AEE85202.1; -; Genomic_DNA.
DR RefSeq; NP_001119062.1; NM_001125590.2.
DR AlphaFoldDB; B3GS44; -.
DR SMR; B3GS44; -.
DR IntAct; B3GS44; 2.
DR STRING; 3702.AT4G26466.1; -.
DR PaxDb; B3GS44; -.
DR PRIDE; B3GS44; -.
DR EnsemblPlants; AT4G26466.1; AT4G26466.1; AT4G26466.
DR GeneID; 6240393; -.
DR Gramene; AT4G26466.1; AT4G26466.1; AT4G26466.
DR KEGG; ath:AT4G26466; -.
DR Araport; AT4G26466; -.
DR TAIR; locus:4515103457; AT4G26466.
DR eggNOG; ENOG502S17V; Eukaryota.
DR HOGENOM; CLU_119747_0_0_1; -.
DR InParanoid; B3GS44; -.
DR OMA; RCKGPAF; -.
DR OrthoDB; 1487552at2759; -.
DR PhylomeDB; B3GS44; -.
DR PRO; PR:B3GS44; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; B3GS44; baseline and differential.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009567; P:double fertilization forming a zygote and endosperm; IMP:TAIR.
DR GO; GO:0010183; P:pollen tube guidance; IMP:TAIR.
DR GO; GO:0010483; P:pollen tube reception; IMP:TAIR.
DR GO; GO:0010198; P:synergid death; IMP:TAIR.
DR InterPro; IPR039307; LORELEI-like.
DR PANTHER; PTHR31533; PTHR31533; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Fertilization; Glycoprotein; GPI-anchor; Lipoprotein;
KW Membrane; Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..139
FT /note="GPI-anchored protein LORELEI"
FT /id="PRO_0000385334"
FT PROPEP 140..165
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000385335"
FT REGION 82..93
FT /note="Required for its function in pollen tube reception"
FT /evidence="ECO:0000269|PubMed:27081182"
FT LIPID 139
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 137
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 82..93
FT /note="Missing: Loss of function in pollen tube reception."
FT /evidence="ECO:0000269|PubMed:27081182"
FT MUTAGEN 139..141
FT /note="STA->T: Loss of localization to the filiform
FT apparatus of synergid cell."
FT /evidence="ECO:0000269|PubMed:27081182"
SQ SEQUENCE 165 AA; 18337 MW; 7C28C3CFDC2D92E4 CRC64;
MELILLFFFL MALLVSLSSS SSISDGVFES QTSVSGRNLR HAKKKCEVNF EYMDYKVLTK
RCKGPAFPAK ECCSAFKEFA CPYVSQINDM NSDCAQTMFS YMNIYGNYPT GLFANECRER
KDGLVCPLPP LYSHNLNAST ADSTPRFISL LISAATAVFA LLVLT
