LRFN2_MACFA
ID LRFN2_MACFA Reviewed; 789 AA.
AC Q9BE71;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Leucine-rich repeat and fibronectin type-III domain-containing protein 2;
DE Flags: Precursor;
GN Name=LRFN2; Synonyms=SALM1; ORFNames=QflA-11865;
OS Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9541;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Frontal cortex;
RA Osada N., Hida M., Kusuda J., Tanuma R., Iseki K., Hirai M., Terao K.,
RA Suzuki Y., Sugano S., Hashimoto K.;
RT "Isolation of full-length cDNA clones from macaque brain cDNA libraries.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Promotes neurite outgrowth in hippocampal neurons. Enhances
CC the cell surface expression of 2 NMDA receptor subunits GRIN1 and
CC GRIN2A. May play a role in redistributing DLG4 to the cell periphery
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms heteromeric complexes with LRFN1, LRFN3, LRFN4 and
CC LRFN5. Can form homomeric complexes, but not across cell junctions.
CC Interacts with 2 NMDA receptor subunits GRIN1 and GRIN2A. Interacts
CC with DLG1, DLG2, DLG3 and DLG4 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC Synapse. Postsynaptic cell membrane {ECO:0000250}.
CC -!- DOMAIN: The PDZ-binding motif is required for cell surface expression,
CC neurite outgrowth promotion and interaction with DLG1, DLG3 and DLG4.
CC {ECO:0000250}.
CC -!- PTM: Glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LRFN family. {ECO:0000305}.
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DR EMBL; AB056799; BAB39323.1; -; mRNA.
DR RefSeq; NP_001270797.1; NM_001283868.1.
DR AlphaFoldDB; Q9BE71; -.
DR SMR; Q9BE71; -.
DR STRING; 9541.XP_005553145.1; -.
DR GeneID; 102141033; -.
DR CTD; 57497; -.
DR eggNOG; KOG0619; Eukaryota.
DR OrthoDB; 151757at2759; -.
DR Proteomes; UP000233100; Unplaced.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR000483; Cys-rich_flank_reg_C.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013098; Ig_I-set.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF07679; I-set; 1.
DR Pfam; PF13855; LRR_8; 2.
DR SMART; SM00409; IG; 1.
DR SMART; SM00408; IGc2; 1.
DR SMART; SM00369; LRR_TYP; 6.
DR SMART; SM00082; LRRCT; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR SUPFAM; SSF49265; SSF49265; 1.
DR PROSITE; PS50853; FN3; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
DR PROSITE; PS51450; LRR; 6.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Leucine-rich repeat; Membrane; Postsynaptic cell membrane;
KW Reference proteome; Repeat; Signal; Synapse; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..789
FT /note="Leucine-rich repeat and fibronectin type-III domain-
FT containing protein 2"
FT /id="PRO_0000014839"
FT TOPO_DOM 21..534
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 535..555
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 556..789
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 21..52
FT /note="LRRNT"
FT REPEAT 53..74
FT /note="LRR 1"
FT REPEAT 77..98
FT /note="LRR 2"
FT REPEAT 101..122
FT /note="LRR 3"
FT REPEAT 125..146
FT /note="LRR 4"
FT REPEAT 150..171
FT /note="LRR 5"
FT REPEAT 174..195
FT /note="LRR 6"
FT REPEAT 198..219
FT /note="LRR 7"
FT DOMAIN 242..288
FT /note="LRRCT"
FT DOMAIN 289..375
FT /note="Ig-like"
FT DOMAIN 421..518
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT REGION 383..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 577..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 619..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 668..702
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 786..789
FT /note="PDZ-binding"
FT COMPBIAS 383..409
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 584..598
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 332
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 341
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 384
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 310..359
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
SQ SEQUENCE 789 AA; 84730 MW; BB86DE81BC284B23 CRC64;
METLLGGLLA FGMAFAVVDA CPKYCVCQNL SESLGTLCPS KGLLFVPPDI DRRTVELRLG
GNFIIHISRQ DFANMTGLVD LTLSRNTISH IQPFSFLDLE SLRSLHLDSN RLPSLGEDTL
RGLVNLQHLI VNNNQLGGIA DEAFEDFLLT LEDLDLSYNN LHGLPWDSVR RMVNLHQLSL
DHNLLDHIAE GTFADLQKLA RLDLTSNRLQ KLPPDPIFAR SQASALTATP FAPPLSFSFG
GNPLHCNCEL LWLRRLERDD DLETCGSPGG LKGRYFWHVR EEEFVCEPPL ITQHTHKLLV
LEGQAATLKC KAIGDPSPLI HWVAPDDRLV GNSSRTAVYD NGTLDIFITT SQDSGAFTCI
AANAAGEATA TVEVSIVQLP HLSNSTSRTA PPKSRLSDIT GSSKTSRGGG GSGGGEPPKS
PPERAVLVSE VTTTSALAKW SVSKSTPRVK MYQLQYNCSD DEVLIYRMIP ASNKAFVVNN
LVSGTGYDLC VLAMWDDTAT TLTATNIVGC AQFFTKADYP QCQSMHSQIL GGTMILVIGG
IIVATLLVFI VILMVRYKVC NHEAPSKMAA AVSNVYSQTN GAQPPPPSSA PAGAPPQGPP
KVVVRNELLD FTASLARASD SSSSSSLGSG EAAGLGRAPW RLPPSAPRPK PSLDRLMGAF
ASLDLKSQRK EELLDSRTPA GRGAGTSARG HHSDREPLLG PPAARARSLL PLPLEGKAKR
SHSFDMGDFA AAAAGGVVPG GYSPPRRVSN IWTKRSLSVN GMLLPFEESD LVGARGTFGS
SEWVMESTV
