ID   LRGA_BACCR              Reviewed;         146 AA.
AC   Q814J2;
DT   29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Antiholin-like protein LrgA {ECO:0000255|HAMAP-Rule:MF_01141};
GN   Name=lrgA {ECO:0000255|HAMAP-Rule:MF_01141}; OrderedLocusNames=BC_5439;
OS   Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC
OS   15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=226900;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC   / NCTC 2599 / NRRL B-3711;
RX   PubMed=12721630; DOI=10.1038/nature01582;
RA   Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V.,
RA   Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M.,
RA   Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G.,
RA   Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.;
RT   "Genome sequence of Bacillus cereus and comparative analysis with Bacillus
RT   anthracis.";
RL   Nature 423:87-91(2003).
CC   -!- FUNCTION: Inhibits the expression or activity of extracellular murein
CC       hydrolases by interacting, possibly with LrgB, with the holin-like
CC       protein CidA. The LrgAB and CidA proteins may affect the proton motive
CC       force of the membrane. May be involved in programmed cell death (PCD),
CC       possibly triggering PCD in response to antibiotics and environmental
CC       stresses. {ECO:0000255|HAMAP-Rule:MF_01141}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01141};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01141}.
CC   -!- SIMILARITY: Belongs to the CidA/LrgA family. LrgA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01141}.
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DR   EMBL; AE016877; AAP12301.1; -; Genomic_DNA.
DR   RefSeq; NP_835100.1; NC_004722.1.
DR   AlphaFoldDB; Q814J2; -.
DR   SMR; Q814J2; -.
DR   STRING; 226900.BC_5439; -.
DR   EnsemblBacteria; AAP12301; AAP12301; BC_5439.
DR   KEGG; bce:BC5439; -.
DR   PATRIC; fig|226900.8.peg.5619; -.
DR   HOGENOM; CLU_113736_0_1_9; -.
DR   OMA; TGWMTQL; -.
DR   Proteomes; UP000001417; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   GO; GO:0012501; P:programmed cell death; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01141; LrgA; 1.
DR   InterPro; IPR023736; Antiholin-like_LrgA.
DR   InterPro; IPR005538; LrgA/CidA.
DR   PANTHER; PTHR33931; PTHR33931; 1.
DR   Pfam; PF03788; LrgA; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cytolysis; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..146
FT                   /note="Antiholin-like protein LrgA"
FT                   /id="PRO_0000213188"
FT   TRANSMEM        9..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01141"
FT   TRANSMEM        35..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01141"
FT   TRANSMEM        64..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01141"
FT   TRANSMEM        93..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01141"
SQ   SEQUENCE   146 AA;  15792 MW;  6BC1CD24DEA7564D CRC64;
     MAKMSTKKVY SFLLQAFIFS AIMLISNIIA THLPIPMPSS VIGLVILFSL LCLKVIKLEQ
     VESLGTALTG IIGFLFVPSG ISVINSLGVM GQYFVQILTV IVVATVILLA VTGLFAQFIL
     GKDEKETEDI KELKVVNKGR KHGKVA