ID   LRGA_BACCZ              Reviewed;         143 AA.
AC   Q630G3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Antiholin-like protein LrgA {ECO:0000255|HAMAP-Rule:MF_01141};
GN   Name=lrgA {ECO:0000255|HAMAP-Rule:MF_01141}; OrderedLocusNames=BCE33L5136;
OS   Bacillus cereus (strain ZK / E33L).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=288681;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ZK / E33L;
RX   PubMed=16621833; DOI=10.1128/jb.188.9.3382-3390.2006;
RA   Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D.,
RA   Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C.,
RA   Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A.,
RA   Hill K.K., Hitchcock P., Jackson P.J., Keim P., Kewalramani A.R.,
RA   Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M.,
RA   Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B.,
RA   Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R.,
RA   Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L.,
RA   Brettin T.S., Gilna P.;
RT   "Pathogenomic sequence analysis of Bacillus cereus and Bacillus
RT   thuringiensis isolates closely related to Bacillus anthracis.";
RL   J. Bacteriol. 188:3382-3390(2006).
CC   -!- FUNCTION: Inhibits the expression or activity of extracellular murein
CC       hydrolases by interacting, possibly with LrgB, with the holin-like
CC       protein CidA. The LrgAB and CidA proteins may affect the proton motive
CC       force of the membrane. May be involved in programmed cell death (PCD),
CC       possibly triggering PCD in response to antibiotics and environmental
CC       stresses. {ECO:0000255|HAMAP-Rule:MF_01141}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01141};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01141}.
CC   -!- SIMILARITY: Belongs to the CidA/LrgA family. LrgA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01141}.
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DR   EMBL; CP000001; AAU20266.1; -; Genomic_DNA.
DR   RefSeq; WP_000104901.1; NZ_CP009968.1.
DR   AlphaFoldDB; Q630G3; -.
DR   EnsemblBacteria; AAU20266; AAU20266; BCE33L5136.
DR   GeneID; 59156929; -.
DR   GeneID; 67469691; -.
DR   KEGG; bcz:BCE33L5136; -.
DR   PATRIC; fig|288681.22.peg.205; -.
DR   OMA; TGWMTQL; -.
DR   Proteomes; UP000002612; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   GO; GO:0012501; P:programmed cell death; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01141; LrgA; 1.
DR   InterPro; IPR023736; Antiholin-like_LrgA.
DR   InterPro; IPR005538; LrgA/CidA.
DR   PANTHER; PTHR33931; PTHR33931; 1.
DR   Pfam; PF03788; LrgA; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cytolysis; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..143
FT                   /note="Antiholin-like protein LrgA"
FT                   /id="PRO_1000065434"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01141"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01141"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01141"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01141"
SQ   SEQUENCE   143 AA;  15424 MW;  4733BC4315DE5996 CRC64;
     MSTKKVYSFL SQAFIFSAIM LISNIIATHL PIPMPSSVIG LVILFSLLCL KVIKLEQVES
     LGTALTGIIG FLFVPSGISV INSLGVMGQY FVQILTVIVV ATVILLAVTG LFAQFILGKD
     EKETEDTKEL KVVNKGRKHG KVA