ID   LRGA_STAA8              Reviewed;         147 AA.
AC   P72358; Q2G1B3;
DT   29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Antiholin-like protein LrgA;
GN   Name=lrgA; OrderedLocusNames=SAOUHSC_00232;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND REGULATION BY LYTR/LYTS.
RX   PubMed=8824633; DOI=10.1128/jb.178.19.5810-5812.1996;
RA   Brunskill E.W., Bayles K.W.;
RT   "Identification of LytSR-regulated genes from Staphylococcus aureus.";
RL   J. Bacteriol. 178:5810-5812(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [3]
RP   FUNCTION.
RX   PubMed=10714982; DOI=10.1128/jb.182.7.1794-1801.2000;
RA   Groicher K.H., Firek B.A., Fujimoto D.F., Bayles K.W.;
RT   "The Staphylococcus aureus lrgAB operon modulates murein hydrolase activity
RT   and penicillin tolerance.";
RL   J. Bacteriol. 182:1794-1801(2000).
RN   [4]
RP   INTERACTION WITH CIDAB.
RX   PubMed=12670989; DOI=10.1128/jb.185.8.2635-2643.2003;
RA   Rice K.C., Firek B.A., Nelson J.B., Yang S.-J., Patton T.G., Bayles K.W.;
RT   "The Staphylococcus aureus cidAB operon: evaluation of its role in
RT   regulation of murein hydrolase activity and penicillin tolerance.";
RL   J. Bacteriol. 185:2635-2643(2003).
RN   [5]
RP   REVIEW.
RX   PubMed=14617136; DOI=10.1046/j.1365-2958.2003.t01-1-03720.x;
RA   Rice K.C., Bayles K.W.;
RT   "Death's toolbox: examining the molecular components of bacterial
RT   programmed cell death.";
RL   Mol. Microbiol. 50:729-738(2003).
CC   -!- FUNCTION: Inhibits the expression or activity of extracellular murein
CC       hydrolases by interacting, possibly with LrgB, with the holin-like
CC       proteins CidA and/or CidB. The LrgAB and CidAB proteins may affect the
CC       proton motive force of the membrane. Increases tolerance to penicillin
CC       possibly by inhibiting the formation of the CidAB holin-like complexes
CC       within the membrane, thus reducing penicillin-induced lethality.
CC       Possibly plays a role in programmed cell death (PCD), triggering PCD in
CC       response to penicillin, and possibly other antibiotics, and
CC       environmental stresses. {ECO:0000269|PubMed:10714982}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Regulated by the two-component system LytR/LytS.
CC   -!- SIMILARITY: Belongs to the CidA/LrgA family. LrgA subfamily.
CC       {ECO:0000305}.
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DR   EMBL; U52961; AAC44839.1; -; Genomic_DNA.
DR   EMBL; CP000253; ABD29407.1; -; Genomic_DNA.
DR   RefSeq; WP_001792906.1; NZ_LS483365.1.
DR   RefSeq; YP_498827.1; NC_007795.1.
DR   AlphaFoldDB; P72358; -.
DR   SMR; P72358; -.
DR   STRING; 1280.SAXN108_0242; -.
DR   TCDB; 1.E.14.1.1; the cida/lrga holin (cida/lrga holin) family.
DR   EnsemblBacteria; ABD29407; ABD29407; SAOUHSC_00232.
DR   GeneID; 3920307; -.
DR   KEGG; sao:SAOUHSC_00232; -.
DR   PATRIC; fig|93061.5.peg.213; -.
DR   eggNOG; COG1380; Bacteria.
DR   HOGENOM; CLU_113736_0_1_9; -.
DR   OMA; TGWMTQL; -.
DR   PRO; PR:P72358; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   GO; GO:0012501; P:programmed cell death; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01141; LrgA; 1.
DR   InterPro; IPR023736; Antiholin-like_LrgA.
DR   InterPro; IPR005538; LrgA/CidA.
DR   PANTHER; PTHR33931; PTHR33931; 1.
DR   Pfam; PF03788; LrgA; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Cytolysis; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..147
FT                   /note="Antiholin-like protein LrgA"
FT                   /id="PRO_0000213195"
FT   TRANSMEM        13..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        42..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   147 AA;  15780 MW;  2F1015429E1F6E47 CRC64;
     MVVKQQKDAS KPAHFFHQVI VIALVLFVSK IIESFMPIPM PASVIGLVLL FVLLCTGAVK
     LGEVEKVGTT LTNNIGLLFV PAGISVVNSL GVISQAPFLI IGLIIVSTIL LLICTGYVTQ
     IIMKVTSRSK GDKVTKKIKI EEAQAHD