ID   LRGB_BACCN              Reviewed;         230 AA.
AC   A7GVI2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Antiholin-like protein LrgB {ECO:0000255|HAMAP-Rule:MF_01142};
GN   Name=lrgB {ECO:0000255|HAMAP-Rule:MF_01142}; OrderedLocusNames=Bcer98_3956;
OS   Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=315749;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 22905 / CIP 110041 / 391-98 / NVH 391-98;
RX   PubMed=17434157; DOI=10.1016/j.cbi.2007.03.003;
RA   Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C.,
RA   Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V.,
RA   Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "Extending the Bacillus cereus group genomics to putative food-borne
RT   pathogens of different toxicity.";
RL   Chem. Biol. Interact. 171:236-249(2008).
CC   -!- FUNCTION: Inhibits the expression or activity of extracellular murein
CC       hydrolases by interacting, possibly with LrgA, with the holin-like
CC       protein CidA. The LrgAB and CidA proteins may affect the proton motive
CC       force of the membrane. May be involved in programmed cell death (PCD),
CC       possibly triggering PCD in response to antibiotics and environmental
CC       stresses. {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01142};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SIMILARITY: Belongs to the CidB/LrgB family. LrgB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000764; ABS24140.1; -; Genomic_DNA.
DR   RefSeq; WP_012096403.1; NC_009674.1.
DR   AlphaFoldDB; A7GVI2; -.
DR   STRING; 315749.Bcer98_3956; -.
DR   EnsemblBacteria; ABS24140; ABS24140; Bcer98_3956.
DR   GeneID; 56419504; -.
DR   KEGG; bcy:Bcer98_3956; -.
DR   eggNOG; COG1346; Bacteria.
DR   HOGENOM; CLU_082099_1_0_9; -.
DR   OMA; PLHTAYA; -.
DR   OrthoDB; 1416872at2; -.
DR   Proteomes; UP000002300; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   GO; GO:0012501; P:programmed cell death; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01142; LrgB; 1.
DR   InterPro; IPR024891; Antiholin-like_LrgB.
DR   InterPro; IPR007300; CidB/LrgB.
DR   PANTHER; PTHR30249; PTHR30249; 1.
DR   Pfam; PF04172; LrgB; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cytolysis; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..230
FT                   /note="Antiholin-like protein LrgB"
FT                   /id="PRO_1000085032"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
SQ   SEQUENCE   230 AA;  24399 MW;  C2C20979FFECCA7D CRC64;
     MASTMTPYFG IVVSLIAYGI GTFLFKHSKQ FFLFTPLFVA MVLGIGFLKV GNFTFEEYNT
     GGKIISFFLE PATIAFAIPL YKQADKLKKY WWQILSAIIV GSICSVVVVF IVAKAIHLDT
     AIMNSMLPQA ATTAIALPLS ESIGGIPAIT SFAVIFNAVI VYALGALFLK TFRVKNPIAK
     GLALGTAGHA LGVAVGIEMG EVEAAMASIA VTVVGVVTVV VIPLFMPLIA