ID   LRGB_BACCR              Reviewed;         230 AA.
AC   Q814J3;
DT   29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Antiholin-like protein LrgB {ECO:0000255|HAMAP-Rule:MF_01142};
GN   Name=lrgB {ECO:0000255|HAMAP-Rule:MF_01142}; OrderedLocusNames=BC_5438;
OS   Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC
OS   15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=226900;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC   / NCTC 2599 / NRRL B-3711;
RX   PubMed=12721630; DOI=10.1038/nature01582;
RA   Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V.,
RA   Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M.,
RA   Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G.,
RA   Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.;
RT   "Genome sequence of Bacillus cereus and comparative analysis with Bacillus
RT   anthracis.";
RL   Nature 423:87-91(2003).
CC   -!- FUNCTION: Inhibits the expression or activity of extracellular murein
CC       hydrolases by interacting, possibly with LrgA, with the holin-like
CC       protein CidA. The LrgAB and CidA proteins may affect the proton motive
CC       force of the membrane. May be involved in programmed cell death (PCD),
CC       possibly triggering PCD in response to antibiotics and environmental
CC       stresses. {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01142};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SIMILARITY: Belongs to the CidB/LrgB family. LrgB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE016877; AAP12300.1; -; Genomic_DNA.
DR   RefSeq; NP_835099.1; NC_004722.1.
DR   RefSeq; WP_000168869.1; NZ_CP034551.1.
DR   AlphaFoldDB; Q814J3; -.
DR   STRING; 226900.BC_5438; -.
DR   EnsemblBacteria; AAP12300; AAP12300; BC_5438.
DR   GeneID; 59156928; -.
DR   GeneID; 64200538; -.
DR   GeneID; 67510063; -.
DR   KEGG; bce:BC5438; -.
DR   PATRIC; fig|226900.8.peg.5618; -.
DR   HOGENOM; CLU_082099_1_0_9; -.
DR   OMA; PLHTAYA; -.
DR   Proteomes; UP000001417; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   GO; GO:0012501; P:programmed cell death; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01142; LrgB; 1.
DR   InterPro; IPR024891; Antiholin-like_LrgB.
DR   InterPro; IPR007300; CidB/LrgB.
DR   PANTHER; PTHR30249; PTHR30249; 1.
DR   Pfam; PF04172; LrgB; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cytolysis; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..230
FT                   /note="Antiholin-like protein LrgB"
FT                   /id="PRO_0000217054"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        32..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        64..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        94..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        143..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        178..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        207..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
SQ   SEQUENCE   230 AA;  24349 MW;  709D16B9CB6B2C4C CRC64;
     MASTMTPYFG IVVSLIAYGI GTLLFKHSKG FFLFTPLFVA MVLGIVFLKV GNFTFEEYNT
     GGKMISFFLE PATIAFAIPL YKQVDKLKKY WWQILSAIVV GSICSVIVVF IVAKAIGLDT
     AVMNSMLPQA ATTAIALPIS ESIGGIPAIT SFAVIFNAVI VYALGALFLK TFRVKHPIAK
     GLALGTAGHA LGVAVGIEMG EVEAAMASIA VTVVGVVTVV VIPMFMPFIG