ID   LRGB_BACCZ              Reviewed;         230 AA.
AC   Q630G4;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Antiholin-like protein LrgB {ECO:0000255|HAMAP-Rule:MF_01142};
GN   Name=lrgB {ECO:0000255|HAMAP-Rule:MF_01142}; OrderedLocusNames=BCE33L5135;
OS   Bacillus cereus (strain ZK / E33L).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=288681;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ZK / E33L;
RX   PubMed=16621833; DOI=10.1128/jb.188.9.3382-3390.2006;
RA   Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D.,
RA   Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C.,
RA   Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A.,
RA   Hill K.K., Hitchcock P., Jackson P.J., Keim P., Kewalramani A.R.,
RA   Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M.,
RA   Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B.,
RA   Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R.,
RA   Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L.,
RA   Brettin T.S., Gilna P.;
RT   "Pathogenomic sequence analysis of Bacillus cereus and Bacillus
RT   thuringiensis isolates closely related to Bacillus anthracis.";
RL   J. Bacteriol. 188:3382-3390(2006).
CC   -!- FUNCTION: Inhibits the expression or activity of extracellular murein
CC       hydrolases by interacting, possibly with LrgA, with the holin-like
CC       protein CidA. The LrgAB and CidA proteins may affect the proton motive
CC       force of the membrane. May be involved in programmed cell death (PCD),
CC       possibly triggering PCD in response to antibiotics and environmental
CC       stresses. {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01142};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SIMILARITY: Belongs to the CidB/LrgB family. LrgB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01142}.
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DR   EMBL; CP000001; AAU20265.1; -; Genomic_DNA.
DR   RefSeq; WP_000168869.1; NZ_CP009968.1.
DR   AlphaFoldDB; Q630G4; -.
DR   EnsemblBacteria; AAU20265; AAU20265; BCE33L5135.
DR   GeneID; 59156928; -.
DR   GeneID; 64200538; -.
DR   GeneID; 67510063; -.
DR   KEGG; bcz:BCE33L5135; -.
DR   PATRIC; fig|288681.22.peg.206; -.
DR   OMA; PLHTAYA; -.
DR   Proteomes; UP000002612; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   GO; GO:0012501; P:programmed cell death; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01142; LrgB; 1.
DR   InterPro; IPR024891; Antiholin-like_LrgB.
DR   InterPro; IPR007300; CidB/LrgB.
DR   PANTHER; PTHR30249; PTHR30249; 1.
DR   Pfam; PF04172; LrgB; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cytolysis; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..230
FT                   /note="Antiholin-like protein LrgB"
FT                   /id="PRO_1000065440"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
SQ   SEQUENCE   230 AA;  24349 MW;  709D16B9CB6B2C4C CRC64;
     MASTMTPYFG IVVSLIAYGI GTLLFKHSKG FFLFTPLFVA MVLGIVFLKV GNFTFEEYNT
     GGKMISFFLE PATIAFAIPL YKQVDKLKKY WWQILSAIVV GSICSVIVVF IVAKAIGLDT
     AVMNSMLPQA ATTAIALPIS ESIGGIPAIT SFAVIFNAVI VYALGALFLK TFRVKHPIAK
     GLALGTAGHA LGVAVGIEMG EVEAAMASIA VTVVGVVTVV VIPMFMPFIG