ID   LRGB_STAA3              Reviewed;         233 AA.
AC   Q2FK07;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Antiholin-like protein LrgB {ECO:0000255|HAMAP-Rule:MF_01142};
GN   Name=lrgB {ECO:0000255|HAMAP-Rule:MF_01142};
GN   OrderedLocusNames=SAUSA300_0257;
OS   Staphylococcus aureus (strain USA300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=367830;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USA300;
RX   PubMed=16517273; DOI=10.1016/s0140-6736(06)68231-7;
RA   Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G.,
RA   Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F.,
RA   Perdreau-Remington F.;
RT   "Complete genome sequence of USA300, an epidemic clone of community-
RT   acquired meticillin-resistant Staphylococcus aureus.";
RL   Lancet 367:731-739(2006).
CC   -!- FUNCTION: Inhibits the expression or activity of extracellular murein
CC       hydrolases by interacting, possibly with LrgA, with the holin-like
CC       proteins CidA and/or CidB. The LrgAB and CidAB proteins may affect the
CC       proton motive force of the membrane. May be involved in programmed cell
CC       death (PCD), possibly triggering PCD in response to antibiotics and
CC       environmental stresses. {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01142};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SIMILARITY: Belongs to the CidB/LrgB family. LrgB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01142}.
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DR   EMBL; CP000255; ABD21434.1; -; Genomic_DNA.
DR   RefSeq; WP_000607067.1; NZ_CP027476.1.
DR   AlphaFoldDB; Q2FK07; -.
DR   EnsemblBacteria; ABD21434; ABD21434; SAUSA300_0257.
DR   KEGG; saa:SAUSA300_0257; -.
DR   HOGENOM; CLU_082099_1_0_9; -.
DR   OMA; PLHTAYA; -.
DR   Proteomes; UP000001939; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   GO; GO:0012501; P:programmed cell death; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01142; LrgB; 1.
DR   InterPro; IPR024891; Antiholin-like_LrgB.
DR   InterPro; IPR007300; CidB/LrgB.
DR   PANTHER; PTHR30249; PTHR30249; 1.
DR   Pfam; PF04172; LrgB; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cytolysis; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..233
FT                   /note="Antiholin-like protein LrgB"
FT                   /id="PRO_1000065443"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        212..232
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
SQ   SEQUENCE   233 AA;  25097 MW;  089E84B7C8246C4E CRC64;
     MINHLALNTP YFGILLSVIP FFLATILFEK TNRFFLFAPL FVSMVFGVAF LYLTGIPYKT
     YKIGGDIIYF FLEPATICFA IPLYKKREVL VKHWHRIIGG IGIGTVVALL IILTFAKLAQ
     FANDVILSML PQAATTAIAL PVSAGIGGIK ELTSLAVILN GVIIYALGNK FLKLFRITNP
     IARGLALGTS GHTLGVAPAK ELGPVEESMA SIALVLVGVV VVAVVPVFVA IFF