ID   LRGB_STAS1              Reviewed;         233 AA.
AC   Q4A012;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Antiholin-like protein LrgB {ECO:0000255|HAMAP-Rule:MF_01142};
GN   Name=lrgB {ECO:0000255|HAMAP-Rule:MF_01142}; OrderedLocusNames=SSP0461;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- FUNCTION: Inhibits the expression or activity of extracellular murein
CC       hydrolases by interacting, possibly with LrgA, with the holin-like
CC       proteins CidA and/or CidB. The LrgAB and CidAB proteins may affect the
CC       proton motive force of the membrane. May be involved in programmed cell
CC       death (PCD), possibly triggering PCD in response to antibiotics and
CC       environmental stresses. {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01142};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01142}.
CC   -!- SIMILARITY: Belongs to the CidB/LrgB family. LrgB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01142}.
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DR   EMBL; AP008934; BAE17606.1; -; Genomic_DNA.
DR   RefSeq; WP_002482419.1; NZ_MTGA01000036.1.
DR   AlphaFoldDB; Q4A012; -.
DR   STRING; 342451.SSP0461; -.
DR   EnsemblBacteria; BAE17606; BAE17606; SSP0461.
DR   KEGG; ssp:SSP0461; -.
DR   PATRIC; fig|342451.11.peg.466; -.
DR   eggNOG; COG1346; Bacteria.
DR   HOGENOM; CLU_082099_1_0_9; -.
DR   OMA; PLHTAYA; -.
DR   OrthoDB; 1416872at2; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   GO; GO:0012501; P:programmed cell death; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01142; LrgB; 1.
DR   InterPro; IPR024891; Antiholin-like_LrgB.
DR   InterPro; IPR007300; CidB/LrgB.
DR   PANTHER; PTHR30249; PTHR30249; 1.
DR   Pfam; PF04172; LrgB; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cytolysis; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..233
FT                   /note="Antiholin-like protein LrgB"
FT                   /id="PRO_0000217065"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
FT   TRANSMEM        212..232
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01142"
SQ   SEQUENCE   233 AA;  24832 MW;  4B38FE2BE4FE6736 CRC64;
     MIEHLAINTP YFGILLSLIP FIIATFLFKK TNGFFLFTPL FVSMVVGIAF LKLTGIDYAN
     YKIGGDIINF FLEPATICFA IPLYKRRDVL KKYWKQILGG ITLGTTAALV CIYLIAEAFQ
     FSNGIIASML PQGATTAIAL PVSADIGGIK ELTSLAVILN GVIIYALGSK LIKLFNITNP
     IARGLALGTS GHSLGVSSAQ EFGETEASMA SISLVIVGVI VVIVAPILAT LLL