LRL1_ARATH
ID LRL1_ARATH Reviewed; 350 AA.
AC Q9ZUG9; Q67YS8; Q8S3D6;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Transcription factor LRL1 {ECO:0000305};
DE AltName: Full=Basic helix-loop-helix protein 66 {ECO:0000303|PubMed:12679534};
DE Short=AtbHLH66 {ECO:0000303|PubMed:12679534};
DE Short=bHLH 66 {ECO:0000303|PubMed:12679534};
DE AltName: Full=Protein DEFECTIVE REGION OF POLLEN 1 {ECO:0000303|PubMed:28585562};
DE AltName: Full=Protein LJRHL1- LIKE 1 {ECO:0000303|PubMed:19675148};
DE Short=AtLRL1 {ECO:0000303|PubMed:19675148};
DE AltName: Full=Transcription factor EN 95 {ECO:0000305};
DE AltName: Full=Transcription factor bHLH66 {ECO:0000305};
DE AltName: Full=bHLH transcription factor bHLH066 {ECO:0000303|PubMed:12679534};
GN Name=LRL1 {ECO:0000303|PubMed:19675148};
GN Synonyms=BHLH66 {ECO:0000303|PubMed:12679534},
GN DROP1 {ECO:0000303|PubMed:28585562}, EN95 {ECO:0000305};
GN OrderedLocusNames=At2g24260 {ECO:0000312|Araport:AT2G24260};
GN ORFNames=F27D4.17 {ECO:0000312|EMBL:AAD03387.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19675148; DOI=10.1104/pp.109.143867;
RA Karas B., Amyot L., Johansen C., Sato S., Tabata S., Kawaguchi M.,
RA Szczyglowski K.;
RT "Conservation of lotus and Arabidopsis basic helix-loop-helix proteins
RT reveals new players in root hair development.";
RL Plant Physiol. 151:1175-1185(2009).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28585562; DOI=10.1038/nplants.2017.79;
RA Zhang J., Huang Q., Zhong S., Bleckmann A., Huang J., Guo X., Lin Q.,
RA Gu H., Dong J., Dresselhaus T., Qu L.J.;
RT "Sperm cells are passive cargo of the pollen tube in plant fertilization.";
RL Nat. Plants 3:17079-17079(2017).
CC -!- FUNCTION: Transcription factor that regulates the development of root
CC hairs (PubMed:19675148). Transcription factor that regulates the
CC development of sperm cells (PubMed:28585562).
CC {ECO:0000269|PubMed:19675148, ECO:0000269|PubMed:28585562}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- INTERACTION:
CC Q9ZUG9; F4I3E6: At1g49830; NbExp=3; IntAct=EBI-15195343, EBI-15195341;
CC Q9ZUG9; Q8S3D1: BHLH68; NbExp=3; IntAct=EBI-15195343, EBI-15192111;
CC Q9ZUG9; Q93Y00: BHLH7; NbExp=3; IntAct=EBI-15195343, EBI-4442198;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- INDUCTION: By cold, UV, ethylene (ACC), flagellin, jasmonic acid (JA),
CC and salicylic acid (SA) treatments. {ECO:0000269|PubMed:12679534}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions (PubMed:19675148, PubMed:28585562). The triple mutant lrl1,
CC lrl2 and lrl3 exhibit very short root hairs (PubMed:19675148). The
CC double mutant drop1 and drop2 fail to develop sperm cells
CC (PubMed:28585562). {ECO:0000269|PubMed:19675148,
CC ECO:0000269|PubMed:28585562}.
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DR EMBL; AF488599; AAM10955.1; -; mRNA.
DR EMBL; AC005967; AAD03387.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07551.1; -; Genomic_DNA.
DR EMBL; AK176370; BAD44133.1; -; mRNA.
DR EMBL; AK176390; BAD44153.1; -; mRNA.
DR EMBL; BT026512; ABH04619.1; -; mRNA.
DR PIR; E84634; E84634.
DR RefSeq; NP_180003.1; NM_127988.6.
DR AlphaFoldDB; Q9ZUG9; -.
DR SMR; Q9ZUG9; -.
DR BioGRID; 2313; 8.
DR IntAct; Q9ZUG9; 8.
DR STRING; 3702.AT2G24260.1; -.
DR PaxDb; Q9ZUG9; -.
DR PRIDE; Q9ZUG9; -.
DR EnsemblPlants; AT2G24260.1; AT2G24260.1; AT2G24260.
DR GeneID; 816961; -.
DR Gramene; AT2G24260.1; AT2G24260.1; AT2G24260.
DR KEGG; ath:AT2G24260; -.
DR Araport; AT2G24260; -.
DR TAIR; locus:2047555; AT2G24260.
DR eggNOG; ENOG502QQDT; Eukaryota.
DR HOGENOM; CLU_044273_0_1_1; -.
DR InParanoid; Q9ZUG9; -.
DR OMA; SDGFHGH; -.
DR OrthoDB; 1415006at2759; -.
DR PhylomeDB; Q9ZUG9; -.
DR PRO; PR:Q9ZUG9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZUG9; baseline and differential.
DR Genevisible; Q9ZUG9; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0055046; P:microgametogenesis; IGI:TAIR.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IGI:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0080147; P:root hair cell development; IMP:TAIR.
DR GO; GO:0048765; P:root hair cell differentiation; IMP:TAIR.
DR GO; GO:0048767; P:root hair elongation; IGI:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..350
FT /note="Transcription factor LRL1"
FT /id="PRO_0000358760"
FT DOMAIN 144..193
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 97..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 144..157
FT /note="Basic motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 158..193
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 219..241
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 318..350
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..131
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 318..343
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 58
FT /note="D -> G (in Ref. 1; AAM10955)"
FT /evidence="ECO:0000305"
FT CONFLICT 268
FT /note="K -> E (in Ref. 1; AAM10955 and 4; BAD44153)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 350 AA; 36522 MW; 8438D16596C636B9 CRC64;
MMNSSLLTPS SSSSSHIQTP STTFDHEDFL DQIFSSAPWP SVVDDAHPLP SDGFHGHDVD
SRNQPIMMMP LNDGSSVHAL YNGFSVAGSL PNFQIPQGSG GGLMNQQGQT QTQTQPQASA
STATGGTVAA PPQSRTKIRA RRGQATDPHS IAERLRRERI AERMKALQEL VPNGNKTDKA
SMLDEIIDYV KFLQLQVKVL SMSRLGGAAS VSSQISEAGG SHGNASSAMV GGSQTAGNSN
DSVTMTEHQV AKLMEEDMGS AMQYLQGKGL CLMPISLATA ISTATCHSRN PLIPGAVADV
GGPSPPNLSG MTIQSTSTKM GSGNGKLNGN GVTERSSSIA VKEAVSVSKA
