LRL2_ARATH
ID LRL2_ARATH Reviewed; 310 AA.
AC Q8S3D5; A0A178UUJ2; C0SVK8; O65552;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Transcription factor LRL2 {ECO:0000305};
DE AltName: Full=Basic helix-loop-helix protein 69 {ECO:0000303|PubMed:12679534};
DE Short=AtbHLH69 {ECO:0000303|PubMed:12679534};
DE Short=bHLH 69 {ECO:0000303|PubMed:12679534};
DE AltName: Full=Protein DEFECTIVE REGION OF POLLEN 2 {ECO:0000303|PubMed:28585562};
DE AltName: Full=Protein LJRHL1- LIKE 2 {ECO:0000303|PubMed:19675148};
DE Short=AtLRL2 {ECO:0000303|PubMed:19675148};
DE AltName: Full=Transcription factor EN 94 {ECO:0000305};
DE AltName: Full=Transcription factor bHLH69 {ECO:0000305};
DE AltName: Full=bHLH transcription factor bHLH069 {ECO:0000303|PubMed:12679534};
GN Name=LRL2 {ECO:0000303|PubMed:19675148};
GN Synonyms=BHLH69 {ECO:0000303|PubMed:12679534},
GN DROP2 {ECO:0000303|PubMed:28585562}, EN94;
GN OrderedLocusNames=At4g30980 {ECO:0000312|Araport:AT4G30980};
GN ORFNames=F6I18.110 {ECO:0000312|EMBL:CAA18195.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Fujita M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19675148; DOI=10.1104/pp.109.143867;
RA Karas B., Amyot L., Johansen C., Sato S., Tabata S., Kawaguchi M.,
RA Szczyglowski K.;
RT "Conservation of lotus and Arabidopsis basic helix-loop-helix proteins
RT reveals new players in root hair development.";
RL Plant Physiol. 151:1175-1185(2009).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28585562; DOI=10.1038/nplants.2017.79;
RA Zhang J., Huang Q., Zhong S., Bleckmann A., Huang J., Guo X., Lin Q.,
RA Gu H., Dong J., Dresselhaus T., Qu L.J.;
RT "Sperm cells are passive cargo of the pollen tube in plant fertilization.";
RL Nat. Plants 3:17079-17079(2017).
CC -!- FUNCTION: Transcription factor that regulates the development of root
CC hairs (PubMed:19675148). Transcription factor that regulates the
CC development of sperm cells (PubMed:28585562).
CC {ECO:0000269|PubMed:19675148, ECO:0000269|PubMed:28585562}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems,
CC and flowers. {ECO:0000269|PubMed:12679534}.
CC -!- INDUCTION: Repressed by heat treatment. {ECO:0000269|PubMed:12679534}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions (PubMed:19675148, PubMed:28585562). The triple mutant lrl1,
CC lrl2 and lrl3 exhibit very short root hairs (PubMed:19675148). The
CC double mutant drop1 and drop2 fail to develop sperm cells
CC (PubMed:28585562). {ECO:0000269|PubMed:19675148,
CC ECO:0000269|PubMed:28585562}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA18195.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79816.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF488601; AAM10956.1; -; mRNA.
DR EMBL; AB493711; BAH30549.1; -; mRNA.
DR EMBL; AL022198; CAA18195.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161578; CAB79816.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85837.1; -; Genomic_DNA.
DR PIR; G85362; G85362.
DR RefSeq; NP_001320095.1; NM_001342047.1.
DR AlphaFoldDB; Q8S3D5; -.
DR SMR; Q8S3D5; -.
DR BioGRID; 14510; 13.
DR IntAct; Q8S3D5; 13.
DR STRING; 3702.AT4G30980.1; -.
DR PaxDb; Q8S3D5; -.
DR PRIDE; Q8S3D5; -.
DR EnsemblPlants; AT4G30980.1; AT4G30980.1; AT4G30980.
DR GeneID; 829223; -.
DR Gramene; AT4G30980.1; AT4G30980.1; AT4G30980.
DR KEGG; ath:AT4G30980; -.
DR Araport; AT4G30980; -.
DR TAIR; locus:2126624; AT4G30980.
DR eggNOG; ENOG502QQDT; Eukaryota.
DR HOGENOM; CLU_044273_0_1_1; -.
DR InParanoid; Q8S3D5; -.
DR PhylomeDB; Q8S3D5; -.
DR PRO; PR:Q8S3D5; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8S3D5; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0055046; P:microgametogenesis; IGI:TAIR.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IGI:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0080147; P:root hair cell development; IMP:TAIR.
DR GO; GO:0048765; P:root hair cell differentiation; IMP:TAIR.
DR GO; GO:0048767; P:root hair elongation; IGI:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..310
FT /note="Transcription factor LRL2"
FT /id="PRO_0000358763"
FT DOMAIN 136..185
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 95..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..149
FT /note="Basic motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 150..185
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 203..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 95..124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 310 AA; 32880 MW; 8C16719A66F7E221 CRC64;
MNSSSLLTPS SSPSPHLQSP ATFDHDDFLH HIFSSTPWPS SVLDDTPPPT SDCAPVTGFH
HHDADSRNQI TMIPLSHNHP NDALFNGFST GSLPFHLPQG SGGQTQTQSQ ATASATTGGA
TAQPQTKPKV RARRGQATDP HSIAERLRRE RIAERMKSLQ ELVPNGNKTD KASMLDEIID
YVKFLQLQVK VLSMSRLGGA ASASSQISED AGGSHENTSS SGEAKMTEHQ VAKLMEEDMG
SAMQYLQGKG LCLMPISLAT TISTATCPSR SPFVKDTGVP LSPNLSTTIV ANGNGSSLVT
VKDAPSVSKP
