LRL3_ARATH
ID LRL3_ARATH Reviewed; 297 AA.
AC Q9LSQ3;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Transcription factor LRL3 {ECO:0000305};
DE AltName: Full=Basic helix-loop-helix protein 82 {ECO:0000303|PubMed:12679534};
DE Short=AtbHLH82 {ECO:0000303|PubMed:12679534};
DE Short=bHLH 82 {ECO:0000303|PubMed:12679534};
DE AltName: Full=Protein DEFECTIVE REGION OF POLLEN 3 {ECO:0000303|PubMed:28585562};
DE AltName: Full=Protein LJRHL1- LIKE 3 {ECO:0000303|PubMed:19675148};
DE Short=AtLRL3 {ECO:0000303|PubMed:19675148};
DE AltName: Full=Transcription factor EN 96 {ECO:0000305};
DE AltName: Full=Transcription factor bHLH82 {ECO:0000305};
DE AltName: Full=bHLH transcription factor bHLH082 {ECO:0000303|PubMed:12679534};
GN Name=LRL3 {ECO:0000303|PubMed:19675148};
GN Synonyms=BHLH82 {ECO:0000303|PubMed:12679534},
GN DROP3 {ECO:0000303|PubMed:28585562}, EN96 {ECO:0000305};
GN OrderedLocusNames=At5g58010 {ECO:0000312|Araport:AT5G58010};
GN ORFNames=F2C19.2 {ECO:0000312|EMBL:BAA97525.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 15-297, TISSUE SPECIFICITY, INDUCTION, GENE
RP FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [6]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=16581911; DOI=10.1073/pnas.0510607103;
RA Lee J.-Y., Colinas J., Wang J.Y., Mace D., Ohler U., Benfey P.N.;
RT "Transcriptional and posttranscriptional regulation of transcription factor
RT expression in Arabidopsis roots.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:6055-6060(2006).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19675148; DOI=10.1104/pp.109.143867;
RA Karas B., Amyot L., Johansen C., Sato S., Tabata S., Kawaguchi M.,
RA Szczyglowski K.;
RT "Conservation of lotus and Arabidopsis basic helix-loop-helix proteins
RT reveals new players in root hair development.";
RL Plant Physiol. 151:1175-1185(2009).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28585562; DOI=10.1038/nplants.2017.79;
RA Zhang J., Huang Q., Zhong S., Bleckmann A., Huang J., Guo X., Lin Q.,
RA Gu H., Dong J., Dresselhaus T., Qu L.J.;
RT "Sperm cells are passive cargo of the pollen tube in plant fertilization.";
RL Nat. Plants 3:17079-17079(2017).
CC -!- FUNCTION: Transcription factor that regulates the development of root
CC hairs (PubMed:19675148). Does not seem to be involved in the regulation
CC of sperm cell development (PubMed:28585562).
CC {ECO:0000269|PubMed:19675148, ECO:0000269|PubMed:28585562}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:16581911}.
CC -!- TISSUE SPECIFICITY: Expressed in trichomes of the root maturation zone.
CC Detected constitutively in flowers. {ECO:0000269|PubMed:12679534,
CC ECO:0000269|PubMed:16581911}.
CC -!- INDUCTION: Repressed by heat treatment. {ECO:0000269|PubMed:12679534}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions (PubMed:19675148, PubMed:28585562). The triple mutant lrl1,
CC lrl2 and lrl3 exhibit very short root hairs (PubMed:19675148).
CC {ECO:0000269|PubMed:19675148, ECO:0000269|PubMed:28585562}.
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DR EMBL; AB026635; BAA97525.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96987.1; -; Genomic_DNA.
DR EMBL; AF488614; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_200609.1; NM_125186.4.
DR AlphaFoldDB; Q9LSQ3; -.
DR SMR; Q9LSQ3; -.
DR BioGRID; 21157; 3.
DR STRING; 3702.AT5G58010.1; -.
DR PaxDb; Q9LSQ3; -.
DR PRIDE; Q9LSQ3; -.
DR ProteomicsDB; 240864; -.
DR EnsemblPlants; AT5G58010.1; AT5G58010.1; AT5G58010.
DR GeneID; 835913; -.
DR Gramene; AT5G58010.1; AT5G58010.1; AT5G58010.
DR KEGG; ath:AT5G58010; -.
DR Araport; AT5G58010; -.
DR TAIR; locus:2147760; AT5G58010.
DR eggNOG; ENOG502QQDT; Eukaryota.
DR HOGENOM; CLU_044273_2_1_1; -.
DR InParanoid; Q9LSQ3; -.
DR OMA; DQFHHPQ; -.
DR OrthoDB; 1415006at2759; -.
DR PhylomeDB; Q9LSQ3; -.
DR PRO; PR:Q9LSQ3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LSQ3; baseline and differential.
DR Genevisible; Q9LSQ3; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0080147; P:root hair cell development; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..297
FT /note="Transcription factor LRL3"
FT /id="PRO_0000358774"
FT DOMAIN 105..154
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 59..109
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 105..118
FT /note="Basic motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 119..154
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT COMPBIAS 68..93
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 287
FT /note="S -> F (in Ref. 3; AF488614)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 297 AA; 31470 MW; AE5451140F11411B CRC64;
MENGNGEGKG EFINQNNDFF LDSMSMLSSL PPCWDPSLPP PPPPPQSLFH ALAVDAPFPD
QFHHPQESGG PTMGSQEGLQ PQGTVSTTSA PVVRQKPRVR ARRGQATDPH SIAERLRRER
IAERMKSLQE LVPNTNKTDK ASMLDEIIEY VRFLQLQVKV LSMSRLGGAG SVGPRLNGLS
AEAGGRLNAL TAPCNGLNGN GNATGSSNES LRSTEQRVAK LMEEDMGSAM QYLQGKGLCL
MPISLATAIS SSTTHSRGSL FNPISSAVAA EDSNVTATAV AAPEASSTMD DVSASKA
