5HT7R_RAT
ID 5HT7R_RAT Reviewed; 448 AA.
AC P32305; P97936;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=5-hydroxytryptamine receptor 7;
DE Short=5-HT-7;
DE Short=5-HT7;
DE AltName: Full=5-HT-X;
DE AltName: Full=GPRFO;
DE AltName: Full=Serotonin receptor 7;
GN Name=Htr7;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
RC STRAIN=Wistar; TISSUE=Brain;
RX PubMed=8397408; DOI=10.1073/pnas.90.18.8547;
RA Martial R., Traiffort E., Leurs R., Tardivel-Lacombe J., Diaz J.,
RA Arrang J.-M., Schwartz J.-C.;
RT "Molecular cloning, characterization, and localization of a high-affinity
RT serotonin receptor (5-HT7) activating cAMP formation.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:8547-8551(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RC STRAIN=Wistar; TISSUE=Brain;
RX PubMed=8517926; DOI=10.1089/dna.1993.12.401;
RA Meyerhof W., Obermueller F., Fehr S., Richter D.;
RT "A novel rat serotonin receptor: primary structure, pharmacology, and
RT expression pattern in distinct brain regions.";
RL DNA Cell Biol. 12:401-409(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
RC STRAIN=Sprague-Dawley; TISSUE=Hypothalamus;
RX PubMed=8398139; DOI=10.1016/0896-6273(93)90149-l;
RA Lovenberg T.W., Baron B.M., de Lecea L., Miller J.D., Prosser R.A.,
RA Rea M.A., Foye P.E., Slone A.L., Siegel B.W., Danielson P.E.,
RA Sutcliffe G.J., Erlander M.G.;
RT "A novel adenylyl cyclase-activating serotonin receptor (5-HT7) implicated
RT in the regulation of mammalian circadian rhythms.";
RL Neuron 11:449-458(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
RC STRAIN=Sprague-Dawley;
RX PubMed=8394362; DOI=10.1016/s0021-9258(17)46830-x;
RA Shen Y., Monsma F.J. Jr., Metcalf M.A., Jose P.A., Hamblin M.W.,
RA Sibley D.R.;
RT "Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin
RT receptor subtype.";
RL J. Biol. Chem. 268:18200-18204(1993).
RN [5]
RP NUCLEOTIDE SEQUENCE (ISOFORM B).
RX PubMed=9084407; DOI=10.1046/j.1471-4159.1997.68041372.x;
RA Heidmann D.E.A., Metcalf M.A., Kohen R., Hamblin M.W.;
RT "Four 5-hydroxytryptamine7 (5-HT7) receptor isoforms in human and rat
RT produced by alternative splicing: species differences due to altered
RT intron-exon organization.";
RL J. Neurochem. 68:1372-1381(1997).
CC -!- FUNCTION: This is one of the several different receptors for 5-
CC hydroxytryptamine (serotonin), a biogenic hormone that functions as a
CC neurotransmitter, a hormone, and a mitogen. The activity of this
CC receptor is mediated by G proteins that stimulate adenylate cyclase.
CC This receptor is implicated in the regulation of mammalian circadian
CC rhythms.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=P32305-1; Sequence=Displayed;
CC Name=B;
CC IsoId=P32305-2; Sequence=VSP_001858;
CC -!- TISSUE SPECIFICITY: Thalamus, hypothalamus, and the hippocampal
CC rudiments.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA42134.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L19654; AAA40617.1; -; Genomic_DNA.
DR EMBL; X69663; CAA49352.1; -; mRNA.
DR EMBL; L22558; AAA42132.1; -; mRNA.
DR EMBL; L15228; AAA42134.1; ALT_INIT; Genomic_DNA.
DR EMBL; U68489; AAB48395.1; -; mRNA.
DR PIR; A47519; A47519.
DR RefSeq; NP_075227.1; NM_022938.2. [P32305-2]
DR RefSeq; XP_006231369.1; XM_006231307.2. [P32305-1]
DR RefSeq; XP_008772122.1; XM_008773900.1. [P32305-1]
DR RefSeq; XP_008772123.1; XM_008773901.1. [P32305-2]
DR AlphaFoldDB; P32305; -.
DR SMR; P32305; -.
DR STRING; 10116.ENSRNOP00000025450; -.
DR BindingDB; P32305; -.
DR ChEMBL; CHEMBL3223; -.
DR DrugCentral; P32305; -.
DR GuidetoPHARMACOLOGY; 12; -.
DR GlyGen; P32305; 2 sites.
DR PhosphoSitePlus; P32305; -.
DR PaxDb; P32305; -.
DR PRIDE; P32305; -.
DR Ensembl; ENSRNOT00000025450; ENSRNOP00000025450; ENSRNOG00000018827. [P32305-2]
DR Ensembl; ENSRNOT00000111885; ENSRNOP00000082600; ENSRNOG00000018827. [P32305-1]
DR GeneID; 65032; -.
DR KEGG; rno:65032; -.
DR UCSC; RGD:71034; rat. [P32305-1]
DR CTD; 3363; -.
DR RGD; 71034; Htr7.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01010000222287; -.
DR HOGENOM; CLU_009579_11_6_1; -.
DR InParanoid; P32305; -.
DR PhylomeDB; P32305; -.
DR Reactome; R-RNO-390666; Serotonin receptors.
DR Reactome; R-RNO-9706019; RHOBTB3 ATPase cycle.
DR PRO; PR:P32305; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000018827; Expressed in brain and 11 other tissues.
DR ExpressionAtlas; P32305; baseline and differential.
DR Genevisible; P32305; RN.
DR GO; GO:0043679; C:axon terminus; IDA:RGD.
DR GO; GO:0030425; C:dendrite; IDA:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099055; C:integral component of postsynaptic membrane; IDA:SynGO.
DR GO; GO:0099056; C:integral component of presynaptic membrane; IDA:SynGO.
DR GO; GO:0043025; C:neuronal cell body; IDA:RGD.
DR GO; GO:0005791; C:rough endoplasmic reticulum; IDA:RGD.
DR GO; GO:0008021; C:synaptic vesicle; IDA:RGD.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IDA:RGD.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IDA:RGD.
DR GO; GO:0007192; P:adenylate cyclase-activating serotonin receptor signaling pathway; IDA:RGD.
DR GO; GO:0048266; P:behavioral response to pain; IMP:RGD.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0007623; P:circadian rhythm; IEA:InterPro.
DR GO; GO:0050966; P:detection of mechanical stimulus involved in sensory perception of pain; IDA:RGD.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:RGD.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR GO; GO:0060291; P:long-term synaptic potentiation; IMP:RGD.
DR GO; GO:0007613; P:memory; IMP:RGD.
DR GO; GO:0060073; P:micturition; IMP:RGD.
DR GO; GO:0042322; P:negative regulation of circadian sleep/wake cycle, REM sleep; IDA:RGD.
DR GO; GO:0014063; P:negative regulation of serotonin secretion; IMP:RGD.
DR GO; GO:0010976; P:positive regulation of neuron projection development; IDA:RGD.
DR GO; GO:2000300; P:regulation of synaptic vesicle exocytosis; IDA:SynGO.
DR GO; GO:0062086; P:regulation of vein smooth muscle contraction; IMP:RGD.
DR GO; GO:0051412; P:response to corticosterone; IDA:RGD.
DR GO; GO:0051602; P:response to electrical stimulus; IEP:RGD.
DR GO; GO:0007210; P:serotonin receptor signaling pathway; IDA:RGD.
DR GO; GO:0014832; P:urinary bladder smooth muscle contraction; IMP:RGD.
DR GO; GO:0042310; P:vasoconstriction; IEA:InterPro.
DR InterPro; IPR001069; 5HT_7_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00652; 5HT7RECEPTR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Lipoprotein; Membrane; Palmitate;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..448
FT /note="5-hydroxytryptamine receptor 7"
FT /id="PRO_0000068981"
FT TOPO_DOM 1..84
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 85..111
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 112..121
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 122..147
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 148..159
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 160..181
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 182..201
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 202..225
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 226..240
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 241..263
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 264..331
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 332..355
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 356..367
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 368..390
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 391..448
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT LIPID 404
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT CARBOHYD 5
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 69
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 158..234
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 436..448
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:8398139"
FT /id="VSP_001858"
FT CONFLICT 110
FT /note="C -> S (in Ref. 4; AAA42134)"
FT /evidence="ECO:0000305"
FT CONFLICT 149
FT /note="G -> D (in Ref. 2; CAA49352)"
FT /evidence="ECO:0000305"
FT CONFLICT 168
FT /note="C -> Y (in Ref. 2; CAA49352)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="R -> S (in Ref. 2; CAA49352)"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="G -> E (in Ref. 2; CAA49352)"
FT /evidence="ECO:0000305"
FT CONFLICT 237
FT /note="S -> N (in Ref. 2; CAA49352)"
FT /evidence="ECO:0000305"
FT CONFLICT 348
FT /note="L -> P (in Ref. 2; CAA49352)"
FT /evidence="ECO:0000305"
FT CONFLICT 396
FT /note="T -> P (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 398
FT /note="Y -> S (in Ref. 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 448 AA; 49837 MW; 746E69C7763B317B CRC64;
MMDVNSSGRP DLYGHLRSLI LPEVGRGLQD LSPDGGAHPV VSSWMPHLLS GFLEVTASPA
PTWDAPPDNV SGCGEQINYG RVEKVVIGSI LTLITLLTIA GNCLVVISVC FVKKLRQPSN
YLIVSLALAD LSVAVAVMPF VSVTDLIGGK WIFGHFFCNV FIAMDVMCCT ASIMTLCVIS
IDRYLGITRP LTYPVRQNGK CMAKMILSVW LLSASITLPP LFGWAQNVND DKVCLISQDF
GYTIYSTAVA FYIPMSVMLF MYYQIYKAAR KSAAKHKFPG FPRVQPESVI SLNGVVKLQK
EVEECANLSR LLKHERKNIS IFKREQKAAT TLGIIVGAFT VCWLPFFLLS TARPFICGTS
CSCIPLWVER TCLWLGYANS LINPFIYAFF NRDLRTTYRS LLQCQYRNIN RKLSAAGMHE
ALKLAERPER SEFVLQNSDH CGKKGHDT
