LRRA_DICDI
ID LRRA_DICDI Reviewed; 510 AA.
AC Q54AX5; Q9NIR8;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Leucine-rich repeat protein lrrA;
GN Name=lrrA; ORFNames=DDB_G0294094;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=AX3-1;
RX PubMed=16051212; DOI=10.1016/j.ydbio.2005.05.045;
RA Liu C.-I., Cheng T.-L., Chen S.-Z., Huang Y.-C., Chang W.-T.;
RT "LrrA, a novel leucine-rich repeat protein involved in cytoskeleton
RT remodeling, is required for multicellular morphogenesis in Dictyostelium
RT discoideum.";
RL Dev. Biol. 285:238-251(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- FUNCTION: Involved in cytoskeleton remodeling, which is needed for
CC normal chemotactic aggregation and efficient cell sorting during
CC multicellular morphogenesis. {ECO:0000269|PubMed:16051212}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16051212}.
CC -!- DEVELOPMENTAL STAGE: Expressed during vegetative growth and up-
CC regulated at early development, with a peak at 6-9 hours of the
CC aggregation stage and then declined abruptly from 16 hours of
CC development. {ECO:0000269|PubMed:16051212}.
CC -!- DISRUPTION PHENOTYPE: Loss of function mutant is defective in the
CC regulation of F-actin organization. {ECO:0000269|PubMed:16051212}.
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DR EMBL; AF200466; AAF66828.1; -; mRNA.
DR EMBL; AAFI02000006; EAL60413.1; -; Genomic_DNA.
DR RefSeq; XP_628849.1; XM_628847.1.
DR AlphaFoldDB; Q54AX5; -.
DR SMR; Q54AX5; -.
DR STRING; 44689.DDB0215361; -.
DR PaxDb; Q54AX5; -.
DR EnsemblProtists; EAL60413; EAL60413; DDB_G0294094.
DR GeneID; 8617996; -.
DR KEGG; ddi:DDB_G0294094; -.
DR dictyBase; DDB_G0294094; lrrA.
DR eggNOG; KOG0619; Eukaryota.
DR HOGENOM; CLU_000288_18_23_1; -.
DR InParanoid; Q54AX5; -.
DR OMA; NQFTSYP; -.
DR PhylomeDB; Q54AX5; -.
DR Reactome; R-DDI-199418; Negative regulation of the PI3K/AKT network.
DR PRO; PR:Q54AX5; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0098609; P:cell-cell adhesion; IMP:dictyBase.
DR GO; GO:0031589; P:cell-substrate adhesion; IMP:dictyBase.
DR GO; GO:0043327; P:chemotaxis to cAMP; IMP:dictyBase.
DR GO; GO:0043326; P:chemotaxis to folate; IMP:dictyBase.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0007163; P:establishment or maintenance of cell polarity; IMP:dictyBase.
DR GO; GO:0030833; P:regulation of actin filament polymerization; IMP:dictyBase.
DR Gene3D; 3.80.10.10; -; 5.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR025875; Leu-rich_rpt_4.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF12799; LRR_4; 1.
DR Pfam; PF13855; LRR_8; 1.
DR SMART; SM00369; LRR_TYP; 14.
DR PROSITE; PS51450; LRR; 19.
PE 1: Evidence at protein level;
KW Cytoplasm; Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..510
FT /note="Leucine-rich repeat protein lrrA"
FT /id="PRO_0000327867"
FT REPEAT 14..34
FT /note="LRR 1"
FT REPEAT 35..59
FT /note="LRR 2"
FT REPEAT 60..82
FT /note="LRR 3"
FT REPEAT 84..106
FT /note="LRR 4"
FT REPEAT 107..130
FT /note="LRR 5"
FT REPEAT 132..152
FT /note="LRR 6"
FT REPEAT 153..176
FT /note="LRR 7"
FT REPEAT 177..200
FT /note="LRR 8"
FT REPEAT 202..222
FT /note="LRR 9"
FT REPEAT 224..245
FT /note="LRR 10"
FT REPEAT 246..270
FT /note="LRR 11"
FT REPEAT 272..292
FT /note="LRR 12"
FT REPEAT 293..315
FT /note="LRR 13"
FT REPEAT 316..340
FT /note="LRR 14"
FT REPEAT 341..363
FT /note="LRR 15"
FT REPEAT 365..386
FT /note="LRR 16"
FT REPEAT 387..408
FT /note="LRR 17"
FT REPEAT 410..432
FT /note="LRR 18"
FT REPEAT 433..458
FT /note="LRR 19"
FT REPEAT 460..478
FT /note="LRR 20"
SQ SEQUENCE 510 AA; 56807 MW; B62713D7AF79FF33 CRC64;
MGGNLSSELK STKYRKREIV DLRKMNIDKL PPTIGALQCK ELLLSENDLI TIPEEIGKLS
KVEIIDFAKN RINYIPPEIG SLATLKQLFL SNNKLFYTPI TPNIGALKNL TRLDLSSNQL
DDLPVEISNC EALEYLDISD NQLQSFPLEF GKLYNLQVFN CSKNSLKSLP SEISGWVKLE
ELNVSNNQLA FLPNQICLLG LLSTLNVGFN KLQQLPEELS SMVSLTNLDL KVNPPLQYVP
QLSNLRQLKI LSIRNLQITH LPLGLGLLSE LIELDIRDNP QLKEIPYDIA TLINLQKLDL
FGNNMRIVPR EVGNLINLQT LDLRQNKLTI DNIPSEIGKL VNLKKLLLSN NLLIALPPEI
ASMKALKEFE ASNNQLQAIP TEIGELSGLT KINLSGNKLT SIPASFGNLS ELQICDLKSN
EIAELPTTLD GLKSCTKIDL SHNMLTELPW EFGDLIGLTI LDVGHNPLTI PPNPIVMKGT
ESIIQWLKKN EKEGRKGKVS GLGIQQDNEK
