LRRT4_HUMAN
ID LRRT4_HUMAN Reviewed; 590 AA.
AC Q86VH4; Q4FZ98; Q6UXJ7;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Leucine-rich repeat transmembrane neuronal protein 4;
DE Flags: Precursor;
GN Name=LRRTM4; ORFNames=UNQ3075/PRO9907;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TISSUE SPECIFICITY.
RX PubMed=12676565; DOI=10.1016/s0888-7543(03)00030-2;
RA Lauren J., Airaksinen M.S., Saarma M., Timmusk T.T.;
RT "A novel gene family encoding leucine-rich repeat transmembrane proteins
RT differentially expressed in the nervous system.";
RL Genomics 81:411-421(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May play a role in the development and maintenance of the
CC vertebrate nervous system. Exhibits strong synaptogenic activity,
CC restricted to excitatory presynaptic differentiation (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Peripherally associated with AMPAR complex. AMPAR complex
CC consists of an inner core made of 4 pore-forming GluA/GRIA proteins
CC (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged
CC in a twofold symmetry. One of the two pairs of distinct binding sites
CC is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors
CC CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR
CC complex is complemented by outer core constituents binding directly to
CC the GluA/GRIA proteins at sites distinct from the interaction sites of
CC the inner core constituents. Outer core constituents include at least
CC PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the
CC inner and outer core serve as a platform for other, more peripherally
CC associated AMPAR constituents, including LRRTM4. Alone or in
CC combination, these auxiliary subunits control the gating and
CC pharmacology of the AMPAR complex and profoundly impact their
CC biogenesis and protein processing (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}. Postsynaptic cell membrane
CC {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q86VH4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q86VH4-2; Sequence=VSP_030989, VSP_030990;
CC -!- TISSUE SPECIFICITY: Expressed in neuronal tissues.
CC {ECO:0000269|PubMed:12676565}.
CC -!- SIMILARITY: Belongs to the LRRTM family. {ECO:0000305}.
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DR EMBL; AY182030; AAO67551.1; -; mRNA.
DR EMBL; AY358324; AAQ88690.1; -; mRNA.
DR EMBL; BC099738; AAH99738.1; -; mRNA.
DR EMBL; BC110058; AAI10059.1; -; mRNA.
DR CCDS; CCDS46346.1; -. [Q86VH4-1]
DR CCDS; CCDS46347.1; -. [Q86VH4-2]
DR RefSeq; NP_001128217.1; NM_001134745.2. [Q86VH4-1]
DR RefSeq; NP_001269853.1; NM_001282924.2. [Q86VH4-1]
DR RefSeq; NP_079269.4; NM_024993.5. [Q86VH4-2]
DR AlphaFoldDB; Q86VH4; -.
DR SMR; Q86VH4; -.
DR BioGRID; 123095; 31.
DR IntAct; Q86VH4; 2.
DR MINT; Q86VH4; -.
DR STRING; 9606.ENSP00000386357; -.
DR TCDB; 8.A.43.1.20; the neat-domain containing methaemoglobin heme sequestration (n-mhs) family.
DR GlyGen; Q86VH4; 3 sites.
DR iPTMnet; Q86VH4; -.
DR PhosphoSitePlus; Q86VH4; -.
DR BioMuta; LRRTM4; -.
DR DMDM; 166897996; -.
DR EPD; Q86VH4; -.
DR jPOST; Q86VH4; -.
DR MassIVE; Q86VH4; -.
DR MaxQB; Q86VH4; -.
DR PaxDb; Q86VH4; -.
DR PeptideAtlas; Q86VH4; -.
DR PRIDE; Q86VH4; -.
DR ProteomicsDB; 70018; -. [Q86VH4-1]
DR ProteomicsDB; 70019; -. [Q86VH4-2]
DR ABCD; Q86VH4; 1 sequenced antibody.
DR Antibodypedia; 47484; 119 antibodies from 28 providers.
DR DNASU; 80059; -.
DR Ensembl; ENST00000409088.3; ENSP00000386236.3; ENSG00000176204.14. [Q86VH4-2]
DR Ensembl; ENST00000409093.1; ENSP00000386357.1; ENSG00000176204.14. [Q86VH4-1]
DR Ensembl; ENST00000409884.6; ENSP00000387297.1; ENSG00000176204.14. [Q86VH4-1]
DR GeneID; 80059; -.
DR KEGG; hsa:80059; -.
DR MANE-Select; ENST00000409884.6; ENSP00000387297.1; NM_001134745.3; NP_001128217.1.
DR UCSC; uc002snq.4; human. [Q86VH4-1]
DR CTD; 80059; -.
DR DisGeNET; 80059; -.
DR GeneCards; LRRTM4; -.
DR HGNC; HGNC:19411; LRRTM4.
DR HPA; ENSG00000176204; Group enriched (brain, retina).
DR MIM; 610870; gene.
DR neXtProt; NX_Q86VH4; -.
DR OpenTargets; ENSG00000176204; -.
DR PharmGKB; PA134972631; -.
DR VEuPathDB; HostDB:ENSG00000176204; -.
DR eggNOG; KOG0619; Eukaryota.
DR GeneTree; ENSGT00940000154909; -.
DR HOGENOM; CLU_032965_0_0_1; -.
DR InParanoid; Q86VH4; -.
DR PhylomeDB; Q86VH4; -.
DR TreeFam; TF332659; -.
DR PathwayCommons; Q86VH4; -.
DR Reactome; R-HSA-6794361; Neurexins and neuroligins.
DR SignaLink; Q86VH4; -.
DR BioGRID-ORCS; 80059; 13 hits in 1069 CRISPR screens.
DR ChiTaRS; LRRTM4; human.
DR GenomeRNAi; 80059; -.
DR Pharos; Q86VH4; Tbio.
DR PRO; PR:Q86VH4; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q86VH4; protein.
DR Bgee; ENSG00000176204; Expressed in secondary oocyte and 105 other tissues.
DR ExpressionAtlas; Q86VH4; baseline and differential.
DR Genevisible; Q86VH4; HS.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0031012; C:extracellular matrix; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00560; LRR_1; 1.
DR Pfam; PF13855; LRR_8; 3.
DR SMART; SM00369; LRR_TYP; 9.
DR PROSITE; PS51450; LRR; 9.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Glycoprotein; Leucine-rich repeat;
KW Membrane; Postsynaptic cell membrane; Reference proteome; Repeat; Signal;
KW Synapse; Transmembrane; Transmembrane helix.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..590
FT /note="Leucine-rich repeat transmembrane neuronal protein
FT 4"
FT /id="PRO_0000018358"
FT TOPO_DOM 31..424
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 446..590
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 31..61
FT /note="LRRNT"
FT REPEAT 62..83
FT /note="LRR 1"
FT REPEAT 86..107
FT /note="LRR 2"
FT REPEAT 110..131
FT /note="LRR 3"
FT REPEAT 134..155
FT /note="LRR 4"
FT REPEAT 158..179
FT /note="LRR 5"
FT REPEAT 182..203
FT /note="LRR 6"
FT REPEAT 206..226
FT /note="LRR 7"
FT REPEAT 230..251
FT /note="LRR 8"
FT REPEAT 254..275
FT /note="LRR 9"
FT REPEAT 278..299
FT /note="LRR 10"
FT DOMAIN 311..362
FT /note="LRRCT"
FT CARBOHYD 58
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 126
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 518
FT /note="M -> V (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12676565,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_030989"
FT VAR_SEQ 519..590
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12676565,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_030990"
SQ SEQUENCE 590 AA; 67217 MW; B7CD270E816CD72F CRC64;
MGFHLITQLK GMSVVLVLLP TLLLVMLTGA QRACPKNCRC DGKIVYCESH AFADIPENIS
GGSQGLSLRF NSIQKLKSNQ FAGLNQLIWL YLDHNYISSV DEDAFQGIRR LKELILSSNK
ITYLHNKTFH PVPNLRNLDL SYNKLQTLQS EQFKGLRKLI ILHLRSNSLK TVPIRVFQDC
RNLDFLDLGY NRLRSLSRNA FAGLLKLKEL HLEHNQFSKI NFAHFPRLFN LRSIYLQWNR
IRSISQGLTW TWSSLHNLDL SGNDIQGIEP GTFKCLPNLQ KLNLDSNKLT NISQETVNAW
ISLISITLSG NMWECSRSIC PLFYWLKNFK GNKESTMICA GPKHIQGEKV SDAVETYNIC
SEVQVVNTER SHLVPQTPQK PLIIPRPTIF KPDVTQSTFE TPSPSPGFQI PGAEQEYEHV
SFHKIIAGSV ALFLSVAMIL LVIYVSWKRY PASMKQLQQH SLMKRRRKKA RESERQMNSP
LQEYYVDYKP TNSETMDISV NGSGPCTYTI SGSRECEMPH HMKPLPYYSY DQPVIGYCQA
HQPLHVTKGY ETVSPEQDES PGLELGRDHS FIATIARSAA PAIYLERIAN
