LRWD1_XENLA
ID LRWD1_XENLA Reviewed; 722 AA.
AC Q6GM71;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Leucine-rich repeat and WD repeat-containing protein 1;
DE AltName: Full=ORC-associated protein;
DE Short=ORCA;
DE AltName: Full=Origin recognition complex-associated protein;
GN Name=lrwd1; Synonyms=orca;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for G1/S transition. Recruits and stabilizes the
CC origin recognition complex (ORC) onto chromatin during G1 to establish
CC pre-replication complex (preRC) and to heterochromatic sites in post-
CC replicated cells. Binds a combination of DNA and histone methylation
CC repressive marks on heterochromatin. Required for silencing of major
CC satellite repeats. May be important ORC2, ORC3 and ORC4 stability (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the ORC complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9UFC0}.
CC Chromosome, centromere {ECO:0000250|UniProtKB:Q9UFC0}. Chromosome,
CC telomere {ECO:0000250|UniProtKB:Q9UFC0}. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q8BUI3}. Chromosome, centromere, kinetochore
CC {ECO:0000250|UniProtKB:Q9UFC0}.
CC -!- DOMAIN: The entire WD repeat region is required for the interaction
CC with ORC complex components, as well as for association with chromatin
CC and for binding to histone methylation marks. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LRWD1 family. {ECO:0000305}.
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DR EMBL; BC074207; AAH74207.1; -; mRNA.
DR RefSeq; NP_001086115.1; NM_001092646.1.
DR AlphaFoldDB; Q6GM71; -.
DR DNASU; 444544; -.
DR GeneID; 444544; -.
DR KEGG; xla:444544; -.
DR CTD; 444544; -.
DR Xenbase; XB-GENE-959485; lrwd1.L.
DR OrthoDB; 810613at2759; -.
DR Proteomes; UP000186698; Chromosome 2L.
DR Bgee; 444544; Expressed in gastrula and 19 other tissues.
DR GO; GO:0000781; C:chromosome, telomeric region; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005721; C:pericentric heterochromatin; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0008327; F:methyl-CpG binding; ISS:UniProtKB.
DR GO; GO:0035064; F:methylated histone binding; ISS:UniProtKB.
DR GO; GO:0006325; P:chromatin organization; ISS:UniProtKB.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0071169; P:establishment of protein localization to chromatin; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51450; LRR; 3.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Centromere; Chromatin regulator; Chromosome; Cytoplasm; Cytoskeleton;
KW DNA replication; Kinetochore; Leucine-rich repeat; Nucleus;
KW Reference proteome; Repeat; Telomere; WD repeat.
FT CHAIN 1..722
FT /note="Leucine-rich repeat and WD repeat-containing protein
FT 1"
FT /id="PRO_0000310996"
FT REPEAT 22..42
FT /note="LRR 1"
FT REPEAT 48..69
FT /note="LRR 2"
FT REPEAT 70..91
FT /note="LRR 3"
FT REPEAT 456..496
FT /note="WD 1"
FT REPEAT 506..546
FT /note="WD 2"
FT REPEAT 565..604
FT /note="WD 3"
FT REPEAT 623..662
FT /note="WD 4"
FT REPEAT 690..722
FT /note="WD 5"
FT REGION 211..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 228..246
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..292
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 722 AA; 82068 MW; 945DBC37CE348DDD CRC64;
MTKLTTELLL KKGLPKCSHL KDLKKLNLSK MQLDLKDLDQ KLFSQMVNLD ELDISHNTLS
DLPGNLRLHN LRILNFADNH VEDVTVLKQF PNLEEVIYED NIYLTVSDNY KVFCLLPKLR
RLNNKDITSL ANHVRFVNHR ELSNRVEAYW ESKYKDKLPD KPPSQKINAV AKDFIKAVVD
NVRYGPSSLK DFVRWKVEMI ARNLIFSLSN DPKKDTDPVL QTSEDTAVEN SSKKRESADE
CTEGSPTKRP RIQIDLQSMP LSPRKSSRLQ NSPLCLTPTK RKQETSAQGT PSKFIETKSP
KIALKTTPSK KHTNELSAKI TGKPKLPLTP KKIHKALDNI EPLHFLQCHS KNNSCEDFKT
QLWACAFEPN LDSSCPKAVA TCGGDSICII DCETGKVMKK YKVTGEEFFT LVWTTLTMIG
KDGQKRKINV LAAGGKYGVV RMIHAKGSLC YGEIKAHKKA ISIMCFSPKQ ETFLFTGSYD
KRIILWDIGV PDCDYNFRPS QLLTLDTTSV PLRMCVVPSC PDEFLVAACE DGCFAWDIGL
DKKQGRRSHE VEFNFPLYKE ERKDKCFHII DSLAFLNEDI IASKSVMQGS IYLWSWEKTL
KSRKNKNVKK LDAVILSQMK WSSSETPYLV LSTSPERDCV FCGDEDGKIW IYDLDSCKAD
LQRGKLCSTV KEPTKILSWP LLCSQKEKTV DKTLINVVTV DPTMEYLVAL TDINIVSIWK
IQ
