LRWD1_XENTR
ID LRWD1_XENTR Reviewed; 691 AA.
AC B0JZ65;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Leucine-rich repeat and WD repeat-containing protein 1;
DE AltName: Full=ORC-associated protein;
DE Short=ORCA;
DE AltName: Full=Origin recognition complex-associated protein;
GN Name=lrwd1; Synonyms=orca;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for G1/S transition. Recruits and stabilizes the
CC origin recognition complex (ORC) onto chromatin during G1 to establish
CC pre-replication complex (preRC) and to heterochromatic sites in post-
CC replicated cells. Binds a combination of DNA and histone methylation
CC repressive marks on heterochromatin. Required for silencing of major
CC satellite repeats. May be important ORC2, ORC3 and ORC4 stability (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the ORC complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9UFC0}.
CC Chromosome, centromere {ECO:0000250|UniProtKB:Q9UFC0}. Chromosome,
CC telomere {ECO:0000250|UniProtKB:Q9UFC0}. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q8BUI3}. Chromosome, centromere, kinetochore
CC {ECO:0000250|UniProtKB:Q9UFC0}.
CC -!- DOMAIN: The entire WD repeat region is required for the interaction
CC with ORC complex components, as well as for association with chromatin
CC and for binding to histone methylation marks. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LRWD1 family. {ECO:0000305}.
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DR EMBL; BC159054; AAI59055.1; -; mRNA.
DR RefSeq; NP_001120130.1; NM_001126658.1.
DR AlphaFoldDB; B0JZ65; -.
DR STRING; 8364.ENSXETP00000012031; -.
DR GeneID; 100145159; -.
DR KEGG; xtr:100145159; -.
DR CTD; 222229; -.
DR Xenbase; XB-GENE-959481; lrwd1.
DR eggNOG; KOG0619; Eukaryota.
DR InParanoid; B0JZ65; -.
DR OrthoDB; 810613at2759; -.
DR Proteomes; UP000008143; Chromosome 2.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000005472; Expressed in gastrula and 15 other tissues.
DR GO; GO:0000781; C:chromosome, telomeric region; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0035098; C:ESC/E(Z) complex; IBA:GO_Central.
DR GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005721; C:pericentric heterochromatin; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0008327; F:methyl-CpG binding; ISS:UniProtKB.
DR GO; GO:0035064; F:methylated histone binding; ISS:UniProtKB.
DR GO; GO:0006325; P:chromatin organization; ISS:UniProtKB.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0071169; P:establishment of protein localization to chromatin; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51450; LRR; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Centromere; Chromatin regulator; Chromosome; Cytoplasm; Cytoskeleton;
KW DNA replication; Kinetochore; Leucine-rich repeat; Nucleus;
KW Reference proteome; Repeat; Telomere; WD repeat.
FT CHAIN 1..691
FT /note="Leucine-rich repeat and WD repeat-containing protein
FT 1"
FT /id="PRO_0000403770"
FT REPEAT 22..45
FT /note="LRR 1"
FT REPEAT 48..69
FT /note="LRR 2"
FT REPEAT 70..91
FT /note="LRR 3"
FT REPEAT 426..466
FT /note="WD 1"
FT REPEAT 476..516
FT /note="WD 2"
FT REPEAT 535..574
FT /note="WD 3"
FT REPEAT 593..632
FT /note="WD 4"
FT REPEAT 659..691
FT /note="WD 5"
FT REGION 202..291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 202..220
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..291
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 691 AA; 78447 MW; 5860175CD129E770 CRC64;
MSKITADVLL KEGLPKSIHL KDLKKLNLSK MHLEMKDIDP KLFSQMVNLD ELDISHNTLS
ELPDNLGLHN LRILNFADNH VEDVTVLKQF PNLEEVIYED NIYLTVSDNY KVFCLLPKLR
RLNNKDITSL ANHVRFVNHR ELSNRVEAHW DSKFKDNLPD KPSSQKINAV AKDFIKSVVN
NIKYGPSSLK EFVRWKALGC SGKKRDSADD CTEGSPTKRT RIQHELQSIP LSPRKSNRLQ
NSPLSLTPIK RKQETSTQGT PSKSTETKSP KVALKSTPSK KQSNESSAKI NGKQKLSLTP
KIIQKALDNI EPLHFLQCHS KNNSCEDFKT QLWACAFEPI LDSSSPKAVA TCGGDSVCII
DCETGKVMKK YKVTGEEFFT LVWTTLTMIG KDEQKRKINV LAAGGKHGVV RIIHAKVSLC
YGEIKAHKKA ISIMCFSPKQ DTFLFTGSYD KRIILWDIGV PDCDYNFRPS QLLTLDTTSV
PLRMCLVPSC PDEFLVAACE DGCFAWDIRL DKKQGRRSYE VELNFPIYKE ERKDNDFHVI
DSLAFLNEDI IASKSVMQGS IYLWSWEKTL KTRKTKNVKK LDAVILAQMK WSSSETPYLV
LSTSPERYCV FCGDEDGKIW IYDLDSCKAD LQRGKLCSVV KEPTKILSWP ILFSPKEKVE
KTLINVVTVD PTMEYLVALT DINIVSIWKI K
