LSAMP_HUMAN
ID LSAMP_HUMAN Reviewed; 338 AA.
AC Q13449; Q8IV49;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Limbic system-associated membrane protein;
DE Short=LSAMP;
DE AltName: Full=IgLON family member 3;
DE Flags: Precursor;
GN Name=LSAMP; Synonyms=IGLON3, LAMP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8666243; DOI=10.1016/0378-1119(96)84698-1;
RA Pimenta A.F., Fischer I., Levitt P.;
RT "cDNA cloning and structural analysis of the human limbic-system-associated
RT membrane protein (LAMP).";
RL Gene 170:189-195(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-300.
RC TISSUE=Leukemic T-cell;
RX PubMed=19349973; DOI=10.1038/nbt.1532;
RA Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA Schiess R., Aebersold R., Watts J.D.;
RT "Mass-spectrometric identification and relative quantification of N-linked
RT cell surface glycoproteins.";
RL Nat. Biotechnol. 27:378-386(2009).
RN [4]
RP VARIANT GLU-225.
RX PubMed=32383616; DOI=10.34172/aim.2020.21;
RA Mehrjoo Z., Kahrizi K., Mohseni M., Akbari M., Arzhangi S., Jalalvand K.,
RA Najmabadi H., Farhadi M., Mohseni M., Asghari A., Mohebbi S., Daneshi A.;
RT "Limbic System Associated Membrane Protein Mutation in an Iranian Family
RT Diagnosed with Meniere's Disease.";
RL Arch. Iran. Med. 23:319-325(2020).
CC -!- FUNCTION: Mediates selective neuronal growth and axon targeting.
CC Contributes to the guidance of developing axons and remodeling of
CC mature circuits in the limbic system. Essential for normal growth of
CC the hippocampal mossy fiber projection (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q13449; Q14457: BECN1; NbExp=3; IntAct=EBI-4314821, EBI-949378;
CC Q13449; Q9HCU0: CD248; NbExp=3; IntAct=EBI-4314821, EBI-9680942;
CC Q13449; Q7Z7K6: CENPV; NbExp=3; IntAct=EBI-4314821, EBI-1210604;
CC Q13449; Q14154: DELE1; NbExp=3; IntAct=EBI-4314821, EBI-2805660;
CC Q13449; P41970: ELK3; NbExp=3; IntAct=EBI-4314821, EBI-1758534;
CC Q13449; Q6NXT2: H3-5; NbExp=3; IntAct=EBI-4314821, EBI-2868501;
CC Q13449; Q13352: ITGB3BP; NbExp=3; IntAct=EBI-4314821, EBI-712105;
CC Q13449; Q9BV99: LRRC61; NbExp=3; IntAct=EBI-4314821, EBI-2350424;
CC Q13449; Q13503: MED21; NbExp=3; IntAct=EBI-4314821, EBI-394678;
CC Q13449; Q8N6F8: METTL27; NbExp=3; IntAct=EBI-4314821, EBI-8487781;
CC Q13449; Q7Z3B1: NEGR1; NbExp=2; IntAct=EBI-4314821, EBI-4314838;
CC Q13449; Q9P121: NTM; NbExp=2; IntAct=EBI-4314821, EBI-4315078;
CC Q13449; Q9BR81: PCDHGC3; NbExp=3; IntAct=EBI-4314821, EBI-22012354;
CC Q13449; Q6P1J6-2: PLB1; NbExp=3; IntAct=EBI-4314821, EBI-10694821;
CC Q13449; Q96GM5: SMARCD1; NbExp=3; IntAct=EBI-4314821, EBI-358489;
CC Q13449; Q92783-2: STAM; NbExp=3; IntAct=EBI-4314821, EBI-12025738;
CC Q13449; Q8WTV1: THAP3; NbExp=3; IntAct=EBI-4314821, EBI-17438286;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
CC -!- TISSUE SPECIFICITY: Expressed on limbic neurons and fiber tracts as
CC well as in single layers of the superior colliculus, spinal cord and
CC cerebellum.
CC -!- SIMILARITY: Belongs to the immunoglobulin superfamily. IgLON family.
CC {ECO:0000305}.
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DR EMBL; U41901; AAC50569.1; -; mRNA.
DR EMBL; BC033803; AAH33803.1; -; mRNA.
DR CCDS; CCDS2982.1; -.
DR PIR; JC4776; JC4776.
DR RefSeq; NP_001305844.1; NM_001318915.1.
DR RefSeq; NP_002329.2; NM_002338.4.
DR AlphaFoldDB; Q13449; -.
DR SMR; Q13449; -.
DR BioGRID; 110223; 5.
DR IntAct; Q13449; 20.
DR STRING; 9606.ENSP00000419000; -.
DR GlyGen; Q13449; 9 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q13449; -.
DR PhosphoSitePlus; Q13449; -.
DR BioMuta; LSAMP; -.
DR DMDM; 116242621; -.
DR EPD; Q13449; -.
DR jPOST; Q13449; -.
DR MassIVE; Q13449; -.
DR MaxQB; Q13449; -.
DR PaxDb; Q13449; -.
DR PeptideAtlas; Q13449; -.
DR PRIDE; Q13449; -.
DR ProteomicsDB; 59454; -.
DR Antibodypedia; 16507; 123 antibodies from 23 providers.
DR DNASU; 4045; -.
DR Ensembl; ENST00000490035.7; ENSP00000419000.1; ENSG00000185565.12.
DR GeneID; 4045; -.
DR KEGG; hsa:4045; -.
DR MANE-Select; ENST00000490035.7; ENSP00000419000.1; NM_002338.5; NP_002329.2.
DR UCSC; uc003ebs.4; human.
DR CTD; 4045; -.
DR DisGeNET; 4045; -.
DR GeneCards; LSAMP; -.
DR HGNC; HGNC:6705; LSAMP.
DR HPA; ENSG00000185565; Tissue enhanced (brain, retina).
DR MIM; 603241; gene.
DR neXtProt; NX_Q13449; -.
DR OpenTargets; ENSG00000185565; -.
DR PharmGKB; PA30470; -.
DR VEuPathDB; HostDB:ENSG00000185565; -.
DR eggNOG; KOG3510; Eukaryota.
DR GeneTree; ENSGT00940000158516; -.
DR InParanoid; Q13449; -.
DR OMA; QCEAVAV; -.
DR OrthoDB; 583722at2759; -.
DR PhylomeDB; Q13449; -.
DR TreeFam; TF325565; -.
DR PathwayCommons; Q13449; -.
DR Reactome; R-HSA-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR SignaLink; Q13449; -.
DR BioGRID-ORCS; 4045; 5 hits in 1062 CRISPR screens.
DR ChiTaRS; LSAMP; human.
DR GenomeRNAi; 4045; -.
DR Pharos; Q13449; Tbio.
DR PRO; PR:Q13449; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q13449; protein.
DR Bgee; ENSG00000185565; Expressed in postcentral gyrus and 145 other tissues.
DR ExpressionAtlas; Q13449; baseline and differential.
DR Genevisible; Q13449; HS.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; TAS:ProtInc.
DR Gene3D; 2.60.40.10; -; 3.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013098; Ig_I-set.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR Pfam; PF07679; I-set; 1.
DR SMART; SM00409; IG; 3.
DR SMART; SM00408; IGc2; 3.
DR SUPFAM; SSF48726; SSF48726; 3.
DR PROSITE; PS50835; IG_LIKE; 3.
PE 1: Evidence at protein level;
KW Cell adhesion; Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor;
KW Immunoglobulin domain; Lipoprotein; Membrane; Phosphoprotein;
KW Reference proteome; Repeat; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..315
FT /note="Limbic system-associated membrane protein"
FT /id="PRO_0000015102"
FT PROPEP 316..338
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000015103"
FT DOMAIN 29..122
FT /note="Ig-like C2-type 1"
FT DOMAIN 132..214
FT /note="Ig-like C2-type 2"
FT DOMAIN 219..304
FT /note="Ig-like C2-type 3"
FT MOD_RES 94
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q8BLK3"
FT LIPID 315
FT /note="GPI-anchor amidated asparagine; alternate"
FT /evidence="ECO:0000255"
FT CARBOHYD 40
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 66
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 136
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 148
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 279
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 287
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 300
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT CARBOHYD 315
FT /note="N-linked (GlcNAc...) asparagine; alternate"
FT /evidence="ECO:0000255"
FT DISULFID 53..111
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 153..197
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 239..290
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VARIANT 225
FT /note="K -> E"
FT /evidence="ECO:0000269|PubMed:32383616"
FT /id="VAR_083713"
FT CONFLICT 3
FT /note="R -> G (in Ref. 1; AAC50569)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="V -> L (in Ref. 1; AAC50569)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 338 AA; 37393 MW; 88CF00E07302817B CRC64;
MVRRVQPDRK QLPLVLLRLL CLLPTGLPVR SVDFNRGTDN ITVRQGDTAI LRCVVEDKNS
KVAWLNRSGI IFAGHDKWSL DPRVELEKRH SLEYSLRIQK VDVYDEGSYT CSVQTQHEPK
TSQVYLIVQV PPKISNISSD VTVNEGSNVT LVCMANGRPE PVITWRHLTP TGREFEGEEE
YLEILGITRE QSGKYECKAA NEVSSADVKQ VKVTVNYPPT ITESKSNEAT TGRQASLKCE
ASAVPAPDFE WYRDDTRINS ANGLEIKSTE GQSSLTVTNV TEEHYGNYTC VAANKLGVTN
ASLVLFRPGS VRGINGSISL AVPLWLLAAS LLCLLSKC
