LSG1_DROME
ID LSG1_DROME Reviewed; 606 AA.
AC Q9W590;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Large subunit GTPase 1 homolog;
DE EC=3.6.1.-;
DE AltName: Full=Nucleostemin-3;
GN Name=Ns3; Synonyms=l(1)G0431; ORFNames=CG14788;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18628395; DOI=10.1101/gad.1670508;
RA Kaplan D.D., Zimmermann G., Suyama K., Meyer T., Scott M.P.;
RT "A nucleostemin family GTPase, NS3, acts in serotonergic neurons to
RT regulate insulin signaling and control body size.";
RL Genes Dev. 22:1877-1893(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276 AND SER-279, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: GTPase required for the nuclear export of the 60S ribosomal
CC subunit. Probably acts by mediating the release of Nmd3 from the 60S
CC ribosomal subunit after export into the cytoplasm. Regulator of body
CC size; acts in serotonergic neurons to regulate insulin signaling and
CC thus exerts global growth control. {ECO:0000269|PubMed:18628395}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18628395}.
CC Note=Punctate distribution.
CC -!- TISSUE SPECIFICITY: Expressed in larval serotonergic neurons.
CC {ECO:0000269|PubMed:18628395}.
CC -!- DOMAIN: In contrast to other GTP-binding proteins, this family is
CC characterized by a circular permutation of the GTPase motifs described
CC by a G4-G1-G3 pattern.
CC -!- DISRUPTION PHENOTYPE: Larvae reach less than 60% of normal size and
CC have fewer and smaller cells, the adults that survive exhibit normal
CC body proportions and are healthy. In the brains excess serotonin and
CC insulin accumulate, while peripheral insulin pathway activation is low.
CC {ECO:0000269|PubMed:18628395}.
CC -!- SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. LSG1
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU01058}.
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DR EMBL; AE014298; AAF45628.1; -; Genomic_DNA.
DR EMBL; AL035632; CAB38462.1; -; Genomic_DNA.
DR EMBL; AY047556; AAK77288.1; -; mRNA.
DR RefSeq; NP_001284779.1; NM_001297850.1.
DR RefSeq; NP_569915.1; NM_130559.3.
DR AlphaFoldDB; Q9W590; -.
DR SMR; Q9W590; -.
DR BioGRID; 57654; 10.
DR IntAct; Q9W590; 2.
DR STRING; 7227.FBpp0070284; -.
DR iPTMnet; Q9W590; -.
DR PaxDb; Q9W590; -.
DR PRIDE; Q9W590; -.
DR DNASU; 31097; -.
DR EnsemblMetazoa; FBtr0070297; FBpp0070284; FBgn0266284.
DR EnsemblMetazoa; FBtr0339578; FBpp0308653; FBgn0266284.
DR GeneID; 31097; -.
DR KEGG; dme:Dmel_CG14788; -.
DR CTD; 31097; -.
DR FlyBase; FBgn0266284; Ns3.
DR VEuPathDB; VectorBase:FBgn0266284; -.
DR eggNOG; KOG1424; Eukaryota.
DR HOGENOM; CLU_011072_2_0_1; -.
DR InParanoid; Q9W590; -.
DR OMA; VNKADMM; -.
DR OrthoDB; 839833at2759; -.
DR PhylomeDB; Q9W590; -.
DR SignaLink; Q9W590; -.
DR BioGRID-ORCS; 31097; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31097; -.
DR PRO; PR:Q9W590; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0266284; Expressed in oviduct (Drosophila) and 37 other tissues.
DR ExpressionAtlas; Q9W590; baseline and differential.
DR Genevisible; Q9W590; DM.
DR GO; GO:0015030; C:Cajal body; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005525; F:GTP binding; ISS:UniProtKB.
DR GO; GO:0003924; F:GTPase activity; IDA:FlyBase.
DR GO; GO:0051168; P:nuclear export; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0046626; P:regulation of insulin receptor signaling pathway; IMP:UniProtKB.
DR GO; GO:0000054; P:ribosomal subunit export from nucleus; IMP:FlyBase.
DR Gene3D; 1.10.1580.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR030378; G_CP_dom.
DR InterPro; IPR043358; GNL1-like.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR023179; GTP-bd_ortho_bundle_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR45709; PTHR45709; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51721; G_CP; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Growth regulation; GTP-binding;
KW Hydrolase; Nucleotide-binding; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..606
FT /note="Large subunit GTPase 1 homolog"
FT /id="PRO_0000324559"
FT DOMAIN 165..395
FT /note="CP-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 574..606
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 213..216
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 344..351
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 388..391
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT MOD_RES 276
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 279
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 606 AA; 70246 MW; 147566CF5CDFB9EB CRC64;
MGKKNKGGAP NLGRQLIKDR FGHTQRRKVD NDTMLHTTEL QDGYDWGRLN LSSVTEESSF
QAFLRTAELA GTEFQAEKLN ITFVNPKQRV GLLSKTQEQR MHQKHDEHRD QLKIPRRPKW
TKETSAEELV RAENEAFLDW RRDLALLQED EEILMTPYEK NLEFWRQLWR VVERSDVVVQ
IVDARNPLLF RSADLERYVK EVEPSKMNMI LVNKSDLLTE EQRRHWAEYF DSEGIRTAFY
SATLVEEELK REAEECLDSF PEVQQLRRAV EEIKQSLDSV EDALNVIEQK YKTIPETQND
ELPRLPGDKN SPRLLSRLEL IEFLRNIYTG PRHTEQHVTV GMVGYPNVGK SSTINSLMTV
KKVSVSATPG KTKRFQTLFL DKDILLCDCP GLVMPSFVLT KADMLLNGIL PIDQMRDHVP
AVNLLCERIP RHVLEDKYGI VIAKPLEGED MERPPHSEEL LLAYGYNRGF MTSNGQPDQA
RSARYVLKDY VNGRLLYAMS PPSVPQTEYH TFPERQRRVI EESQLPGQQQ RAMRINKSTS
KELDNQFFSD KPTHAHVKGR TNFPNVRLAN DGSLVAGNDP AAKPWRHVKK ERREKLRKKF
SHLDEH
