LSG1_YEAST
ID LSG1_YEAST Reviewed; 640 AA.
AC P53145; D6VU46;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=Large subunit GTPase 1;
DE EC=3.6.1.-;
GN Name=LSG1; OrderedLocusNames=YGL099W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9290212;
RX DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT chromosome VII.";
RL Yeast 13:1077-1090(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH 60S RIBOSOMAL SUBUNIT.
RX PubMed=12773575; DOI=10.1128/mcb.23.12.4344-4355.2003;
RA Kallstrom G., Hedges J., Johnson A.;
RT "The putative GTPases Nog1p and Lsg1p are required for 60S ribosomal
RT subunit biogenesis and are localized to the nucleus and cytoplasm,
RT respectively.";
RL Mol. Cell. Biol. 23:4344-4355(2003).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION, AND MUTAGENESIS OF ILE-204 AND LYS-349.
RX PubMed=15660131; DOI=10.1038/sj.emboj.7600547;
RA Hedges J., West M., Johnson A.W.;
RT "Release of the export adapter, Nmd3p, from the 60S ribosomal subunit
RT requires Rpl10p and the cytoplasmic GTPase Lsg1p.";
RL EMBO J. 24:567-579(2005).
RN [8]
RP FUNCTION.
RX PubMed=15831484; DOI=10.1128/mcb.25.9.3802-3813.2005;
RA West M., Hedges J.B., Chen A., Johnson A.W.;
RT "Defining the order in which Nmd3p and Rpl10p load onto nascent 60S
RT ribosomal subunits.";
RL Mol. Cell. Biol. 25:3802-3813(2005).
RN [9]
RP INTERACTION WITH ARB1.
RX PubMed=16260602; DOI=10.1128/mcb.25.22.9859-9873.2005;
RA Dong J., Lai R., Jennings J.L., Link A.J., Hinnebusch A.G.;
RT "The novel ATP-binding cassette protein ARB1 is a shuttling factor that
RT stimulates 40S and 60S ribosome biogenesis.";
RL Mol. Cell. Biol. 25:9859-9873(2005).
RN [10]
RP FUNCTION.
RX PubMed=17015443; DOI=10.1074/jbc.m606798200;
RA Hedges J., Chen Y.I., West M., Bussiere C., Johnson A.W.;
RT "Mapping the functional domains of yeast NMD3, the nuclear export adapter
RT for the 60 S ribosomal subunit.";
RL J. Biol. Chem. 281:36579-36587(2006).
RN [11]
RP FUNCTION.
RX PubMed=17761675; DOI=10.1074/jbc.m705057200;
RA Hofer A., Bussiere C., Johnson A.W.;
RT "Mutational analysis of the ribosomal protein Rpl10 from yeast.";
RL J. Biol. Chem. 282:32630-32639(2007).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: GTPase required for the nuclear export of the 60S ribosomal
CC subunit. Acts by mediating the release of NMD3 from the 60S ribosomal
CC subunit after export into the cytoplasm. {ECO:0000269|PubMed:15660131,
CC ECO:0000269|PubMed:15831484, ECO:0000269|PubMed:17015443,
CC ECO:0000269|PubMed:17761675}.
CC -!- SUBUNIT: Associates with the 60S ribosomal subunit. Interacts with
CC ARB1. {ECO:0000269|PubMed:12773575, ECO:0000269|PubMed:16260602}.
CC -!- INTERACTION:
CC P53145; P38344: REI1; NbExp=5; IntAct=EBI-23885, EBI-21136;
CC P53145; Q12522: TIF6; NbExp=5; IntAct=EBI-23885, EBI-9046;
CC P53145; Q02256: YVH1; NbExp=3; IntAct=EBI-23885, EBI-14322;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12773575,
CC ECO:0000269|PubMed:14562095}.
CC -!- DOMAIN: In contrast to other GTP-binding proteins, this family is
CC characterized by a circular permutation of the GTPase motifs described
CC by a G4-G1-G3 pattern.
CC -!- MISCELLANEOUS: Present with 19400 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. LSG1
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU01058}.
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DR EMBL; Z72621; CAA96805.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08007.1; -; Genomic_DNA.
DR PIR; S64106; S64106.
DR RefSeq; NP_011416.3; NM_001180964.3.
DR PDB; 5T62; EM; 3.30 A; W=1-640.
DR PDB; 6N8N; EM; 3.80 A; W=1-640.
DR PDB; 6N8O; EM; 3.50 A; W=1-640.
DR PDB; 6QIK; EM; 3.10 A; o=1-640.
DR PDB; 6QTZ; EM; 3.50 A; o=1-640.
DR PDB; 6RI5; EM; 3.30 A; o=1-640.
DR PDB; 6RZZ; EM; 3.20 A; o=1-640.
DR PDB; 6S05; EM; 3.90 A; o=1-640.
DR PDBsum; 5T62; -.
DR PDBsum; 6N8N; -.
DR PDBsum; 6N8O; -.
DR PDBsum; 6QIK; -.
DR PDBsum; 6QTZ; -.
DR PDBsum; 6RI5; -.
DR PDBsum; 6RZZ; -.
DR PDBsum; 6S05; -.
DR AlphaFoldDB; P53145; -.
DR SMR; P53145; -.
DR BioGRID; 33150; 146.
DR DIP; DIP-6659N; -.
DR IntAct; P53145; 37.
DR MINT; P53145; -.
DR STRING; 4932.YGL099W; -.
DR iPTMnet; P53145; -.
DR MaxQB; P53145; -.
DR PaxDb; P53145; -.
DR PRIDE; P53145; -.
DR EnsemblFungi; YGL099W_mRNA; YGL099W; YGL099W.
DR GeneID; 852779; -.
DR KEGG; sce:YGL099W; -.
DR SGD; S000003067; LSG1.
DR VEuPathDB; FungiDB:YGL099W; -.
DR eggNOG; KOG1424; Eukaryota.
DR GeneTree; ENSGT00940000156442; -.
DR HOGENOM; CLU_011072_2_0_1; -.
DR InParanoid; P53145; -.
DR OMA; VNKADMM; -.
DR BioCyc; YEAST:G3O-30599-MON; -.
DR PRO; PR:P53145; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53145; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IDA:SGD.
DR GO; GO:0043332; C:mating projection tip; HDA:SGD.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; ISS:SGD.
DR GO; GO:0003729; F:mRNA binding; HDA:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IMP:SGD.
DR GO; GO:0000054; P:ribosomal subunit export from nucleus; IMP:SGD.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR030378; G_CP_dom.
DR InterPro; IPR043358; GNL1-like.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR45709; PTHR45709; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51721; G_CP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; GTP-binding; Hydrolase; Nucleotide-binding;
KW Phosphoprotein; Protein transport; Reference proteome; Transport.
FT CHAIN 1..640
FT /note="Large subunit GTPase 1"
FT /id="PRO_0000122454"
FT DOMAIN 188..394
FT /note="CP-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 607..640
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 616..630
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 236..239
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 343..350
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000305"
FT BINDING 387..390
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT MOD_RES 103
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MUTAGEN 204
FT /note="I->T: Impairs the nuclear export of 60S ribosomal
FT subunit in cytoplasm."
FT /evidence="ECO:0000269|PubMed:15660131"
FT MUTAGEN 349
FT /note="K->T: Dominant negative mutant; prevents the nuclear
FT export of 60S ribosomal subunit in cytoplasm."
FT /evidence="ECO:0000269|PubMed:15660131"
SQ SEQUENCE 640 AA; 72728 MW; 15FD76541A2E8410 CRC64;
MPPKEAPKKW KAPKGPKPTH RKNKNKLELG RAIKYARQKE NAIEYLPDGE MRFTTDKHEA
NWVKLRSVTQ ESALDEFLST AALADKDFTA DRHSNVKIIR MDSGNDSATS QGFSMTNEQR
GNLNAKQRAL AKDLIVPRRP EWNEGMSKFQ LDRQEKEAFL EWRRKLAHLQ ESNEDLLLTP
FERNIEVWKQ LWRVVERSDL VVQIVDARNP LLFRSVDLER YVKESDDRKA NLLLVNKADL
LTKKQRIAWA KYFISKNISF TFYSALRANQ LLEKQKEMGE DYREQDFEEA DKEGFDADEK
VMEKVKILSI DQLEELFLSK APNEPLLPPL PGQPPLINIG LVGYPNVGKS STINSLVGAK
KVSVSSTPGK TKHFQTIKLS DSVMLCDCPG LVFPNFAYNK GELVCNGVLP IDQLRDYIGP
AGLVAERIPK YYIEAIYGIH IQTKSRDEGG NGDIPTAQEL LVAYARARGY MTQGYGSADE
PRASRYILKD YVNGKLLYVN PPPHLEDDTP YTREECEEFN KDLYVFDRLP DTRKEQVQNA
AKAKGIDIVD LARDLNQLTF SAHTGGDTQK EAKSVTHGGK QAALYNAAED LDRDFFKMNN
VEGRLSTPFH KVQNSSAGKR HNKKNKSKNA KSKVFSIENN
